Search results for "MALDI-TOF M"
showing 10 items of 20 documents
Identification of cryptic Candida species by MALDI-TOF mass spectrometry, not all MALDI-TOF systems are the same: focus on the C. parapsilosis specie…
2016
Data about the performance of MALDI-TOF mass spectrometry against cryptic Candida species are limited. According to our findings within the C. parapsilosis species complex, microbiologists should be aware that the choice of the instrument is critical for accurate species identification due to the risk of misidentification in the clinical setting.
Description of Klebsiella spallanzanii sp. nov. and of Klebsiella pasteurii sp. nov
2019
AbstractKlebsiella oxytocacauses opportunistic human infections and post-antibiotic haemorrhagic diarrhoea. ThisEnterobacteriaceaespecies is genetically heterogeneous and is currently subdivided into seven phylogroups (Ko1 to Ko4, Ko6 to Ko8). Here we investigated the taxonomic status of phylogroups Ko3 and Ko4. Genomic sequence-based phylogenetic analyses demonstrate that Ko3 and Ko4 formed well-defined sequence clusters related to, but distinct from,Klebsiella michiganensis(Ko1),Klebsiella oxytoca(Ko2),K. huaxiensis(Ko8) andK. grimontii(Ko6). The average nucleotide identity of Ko3 and Ko4 were 90.7% withK. huaxiensisand 95.5% withK. grimontii, respectively. In addition, three strains ofK.…
Development of a Novel Method for Identification of Alaria alata Mesocercariae by Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spe…
2021
Alaria (A.) alata mesocercariae (AM) have increasingly appeared as incidental findings during the mandatory inspection of wild boars for Trichinella in many European countries. An Alaria spp.-specific PCR is available for the identification of AM
Comparative Quantitative Analysis of Porcine Optic Nerve Head and Retina Subproteomes
2019
Optic nerve head (ONH) and retina (RET) are the main sites of damage in neurodegenerative optic neuropathies including glaucoma. Up to date, little is known about the molecular interplay between these two adjoining ocular components in terms of proteomics. To close this gap, we investigated ONH and RET protein extracts derived from porcine eyes (n = 12) (Sus scrofa domestica Linnaeus 1758) using semi-quantitative mass spectrometry (MS)-based proteomics comprising bottom-up LC&ndash
Assessing the MALDI-TOF MS sample preparation procedure to analyze the influence of thermo-oxidative ageing and thermo-mechanical degradation on poly…
2011
[EN] Multiple processing by means of successive injection cycles was used to simulate the thermo-mechanical degradation effects on the oligomeric distribution of PLA under mechanical recycling. Likewise, an accelerated thermal ageing over PLA glass transition was performed in order to simulate its service life. MALDI-TOF MS was used for the analysis and the sample preparation procedure was assessed by means of a statistical Design of Experiments (DoE). The quality effects in use for the analysis were signal-to-noise ratio and Resolution. Different matrixes, analyte/matrix proportions and the use of NaTFA as cationization agent were considered. A deep inspection of the statistical results pr…
New amphiphilic conjugates of mono- and bis(carboxy)-PEG(2,000) polymers with lipoamino acids as surface modifiers of colloidal drug carriers
2010
Nuevas aplicaciones del sistema MALDI-TOF MS en la Microbiología Clínica
2022
La espectrometría de masas es una técnica de determinación estructural que permite estudiar la distribución de las moléculas de una sustancia en función de su masa. Un espectro de masas es una relación de las especies iónicas presentes en una muestra, expresadas en función de su masa/carga (m/z) y la abundancia relativa (intensidad) de cada una en la muestra. La espectrometría de masas fue utilizada históricamente como una técnica analítica de la química clínica, pero no fue hasta hace tres décadas con la aparición de las técnicas de “ionización suave” cuando se consiguió analizar biomoléculas de gran tamaño utilizando un láser como fuente de ionización y una matriz orgánica para facilitar …
Legionella pneumophila identifikācija, izmantojot MALDI-TOF MS un SBT metodes
2016
Legionella pneumophila identifikācijai un subtipēšanai tika pielietotas divas metodes – MALDI-TOF MS un SBT. Tika izmantoti 22 no ūdens izolēti ZI ”BIOR” Legionella pneumophila celmi. Ar lateksa aglutinācijas metodi tika noteiktas izmantojamās Legionella pneumophila serogrupas – 1, 2, 3 un 6. MALDI-TOF MS ekstrakcijas metode nodrošināja 100 % identifikāciju visiem Legionella pneumophila izolātiem. Pēc 17 iegūtajiem masas spektra signāliem celmi tika efektīvi subtipēti. Ar SBT metodi celmiem tika iegūti alēļu profili un sekvences tipa numuri, kopumā 15 dažādi Legionella pneumophila ST, no kuriem divi – ST2191 un ST2192 iepriekš nebija iekļauti starptautiskajā ESGLI datubāzē. Abu metožu iegūt…
Influence of a Thiolate Chemical Layer on GaAs (100) Biofunctionalization: An Original Approach Coupling Atomic Force Microscopy and Mass Spectrometr…
2013
International audience; Widely used in microelectronics and optoelectronics; Gallium Arsenide (GaAs) is a III-V crystal with several interesting properties for microsystem and biosensor applications. Among these; its piezoelectric properties and the ability to directly biofunctionalize the bare surface, offer an opportunity to combine a highly sensitive transducer with a specific bio-interface; which are the two essential parts of a biosensor. To optimize the biorecognition part; it is necessary to control protein coverage and the binding affinity of the protein layer on the GaAs surface. In this paper; we investigate the potential of a specific chemical interface composed of thiolate molec…
Legionella pneumophila izplatība un sekvenču tipu daudzveidība Latvijas viesnīcu ūdensapgādes sistēmās
2018
Legionella pneumophila ir nosacīti patogēna, dabiskās un mākslīgi radītās saldūdens ūdenskrātuvēs sastopama baktērija, kas spēj izraisīt infekcijas slimību – legionelozi. Lai noskaidrotu L. pneumophila izplatību Latvijā, no 40 viesnīcu ūdensapgādes sistēmām noņemti 240 karstā ūdens paraugi un testēti uz L. pneumophila klātbūtni. No pētījumā iesaistītajām viesnīcām 25 (65 %) viesnīcās konstatēts vismaz viens Legionella pozitīvs paraugs. Maksimālais L. pneumophila koloniju veidojošo vienību skaits vienā paraugā bija 1,1 x 104 kvv/l. Ar MALDI-TOF MS apstiprināti 99 L. pneumophila un 9 L. rubrilucens izolāti. Pārstāvētākā no serogrupām bija L. pneumophila serogrupa 3 (68 izolāti). Izmantojot SB…