Search results for "MALDI"

Revisited BIA-MS combination: Entire "on-a-chip" processing leading to the proteins identification at low femtomole to sub-femtomole levels

2008

International audience; We present the results of a study in which biomolecular interaction analysis (BIA, Biacore 2000) was combined with mass spectrometry (MS) using entire "on-a-chip" procedure. Most BIA-MS studies included an elution step of the analyte prior MS analysis. Here, we report a low-cost approach combining Biacore analysis with homemade chips and MS in situ identification onto the chips without elution step. First experiments have been made with rat serum albumin to determine the sensitivity and validation of the concept has been obtained with an antibody/antigen couple. Our "on-a-chip" procedure allowed complete analysis by MS-MS of the biochip leading to protein identificat…

Cine, género e imaginarios nacionales: la representación cinematográfica de España en "La aldea maldita" (F. Rey, 1930)

2006

Lactose Intolerance and Self-Reported Milk Intolerance: Relationship with Lactose Maldigestion and Nutrient Intake

1998

Background: The relationship between lactose-maldigestion, self-reported milk intolerance and gastrointestinal symptoms has not been clearly defined.Objectives: To evaluate: a) the prevalence of lactose maldigestion and lactose intolerance in a sample of the general population taken from a rural center; b) the frequency of self-reported milk-intolerance and its correlation with lactose-maldigestion; c) the influence of lactose maldigestion, lactose intolerance and self-reported milk intolerance on dietary habits and consumption of total calories, protein, and calcium.Subjects: We studied a randomized sample of the general population in a small center in Sicily. 323 subjects (150 males, 173 …

Multi-proxy analysis suggests Late Pleistocene affinities of human skeletal remains attributed to Balzi Rossi

2021

: In two publications from 1967 and 1971, M. Masali described human skeletal remains presumed to have been found in the Balzi Rossi caves (Ventimiglia, Italy), based on a signed note dated to 1908. Since then, the remains - dubbed "Conio's Finds" and preserved at the University of Torino - had not been further studied. We performed a multidisciplinary investigation aimed at clarifying the geographical and chronological attribution of these specimens. Collagen extraction for AMS dating was unsuccessful, but we obtained two direct dates on the best- preserved crania via 231Pa/235U direct gamma-ray spectrometry (10,500±2,000 years BP and 12,500±2,500 years BP). We analyzed the metrics and morp…

Traduire Henri Maldiney : conditions d’une réception en anglais

2019

Cet article porte sur la traduction multilingue de l’œuvre du philosophe français Henri Maldiney (1912-2013). Quelles sont les conditions de traduction d’une œuvre philosophique nourrie de multiples corpus et transdisciplinaire ? Plus précisément, nous tentons de répondre aux questions suivantes : pourquoi traduire Maldiney ? Qui traduit Maldiney ? Chez quel éditeur ? À partir de quel corpus, si l’on songe que ses œuvres complètes sont en cours de réédition depuis 2012 ? Cette réédition et l’arrivée d’une nouvelle génération de lecteurs ont stimulé des traductions partielles en allemand, italien, japonais, russe et portugais, assorties de réflexions traductologiques. Les diverses traduction…

Core oligosaccharide of Escherichia coli B—the structure required for bacteriophage T4 recognition

2015

Abstract The structure of Escherichia coli B strain PCM 1935 core oligosaccharide has been investigated by 1H and 13C NMR spectroscopy, MALDI-TOF MS and ESI MSn. It was concluded that the core oligosaccharide is a pentasaccharide with the following structure: ESI MS/MS analysis revealed that the glycine (a minor component) is linked to the →3,7)- l -α- d -Hepp-(1→ residue.

El sirventès en la poesia catalana dels segles XIV-XV : un catàleg

2018

The sirventes of the troubadour era has been broadly studied, but there are not overall explanations concerning the evolution of the sirventes genre in the Catalan-speaking lands of the fourteenth and fifteenth centuries. This paper propounds a catalogue of sirventesos concerning its historical development, the troubadour models, rhetorical and grammatical treatises, and genre assignment in rubrics. Our catalogue contains thirty-one Catalan sirventesos , including twenty-eight extant items and three items lost. In some cases the literary motifs of the sirventes cross boundaries in a process of poetic hybridization with other moral genres which borrow out themes, images and rhetorical strate…

A laser desorption/resonance enhanced photoionisation TOF-system for the spatially resolved trace analysis of elements

2004

Abstract A novel method for direct and spatially resolved elemental trace analysis with high sensitivity and elemental selectivity is presented. The concept is based on the combination of a commercial MALDI-TOF mass spectrometer with a pulsed laser system for resonant postionisation of neutrals. While the MALDI method is usually applied for investigations of large organic compounds and biomolecules, the technique discussed here concerns the low mass range around 1 ⩽ A ⩽ 300. The analytical performances of the setup with respect to mass analysis, spatial resolution and overall detection efficiency are discussed.

Comparison of two methods skipping cell lysis and protein extraction for identification of bacteria from blood cultures by matrix-assisted laser deso…

2019

ABSTRACT Objective Matrix-assisted laser desorption/ionization time-of-flight mass-spectrometry (MALDI-TOF MS) is widely used for fast identification of bacteria from blood cultures (BC). We compared the performance of two procedures, one including a pre-enrichment step in brain heart infusion and the other a direct method using vacutainer separator gel tubes (DI), for identification of bacteria from blood cultures by MALDI-TOF MS. Material and methods We first prepared a training set of 20 simulated bacteremia specimens, including 10 Gram-negative and 10 Gram-positive species. A total of 145 non-consecutive BCs flagged as positive (68 Gram-negative rods, and 77 Gram-positive cocci) were pr…

Using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry for the characterization of functionalized carbon nanotubes

2013

RATIONALE: Functionalization of carbon nanotubes (CNTs) generates complex systems that require the development of suitable characterization protocols. New techniques have been explored, and existing analytical and spectroscopic methods to characterize functionalized CNTs have been adapted. Presently, chemical characterization of functionalized CNTs (f-CNTs) remains a difficult task. METHODS: Matrix-assisted laser desorption/ionization (MALDI) analysis is performed on f-MWCNT samples prepared via grafting or absorption of anti-oxidant (AO) molecules on both MWCNT-COOH and MWCNT-OH. Covalently functionalized MWCNTs were subjected to thermal degradation and/or hydrolysis reaction before analys…