Search results for "MEF"

Class IIa HDACs repressive activities on MEF2-depedent transcription are associated with poor prognosis of ER⁺ breast tumors.

2013

MEF2s transcription factors and class IIa HDACs compose a fundamental axis for several differentiation pathways. Functional relationships between this axis and cancer are largely unexplored. We have found that class IIa HDACs are heterogeneously expressed and display redundant activities in breast cancer cells. Applying gene set enrichment analysis to compare the expression profile of a list of putative MEF2 target genes, we have discovered a correlation between the down-regulation of the MEF2 signature and the aggressiveness of ER(+) breast tumors. Kaplan-Meier analysis in ER(+) breast tumors evidenced an association between increased class IIa HDACs expression and reduced survival. The im…

Temporal coherency between receptor expression, neural activity and AP-1-dependent transcription regulates Drosophila motoneuron dendrite development.

2013

Neural activity has profound effects on the development of dendritic structure. Mechanisms that link neural activity to nuclear gene expression include activity-regulated factors, such as CREB, Crest or Mef2, as well as activity-regulated immediate-early genes, such as fos and jun. This study investigates the role of the transcriptional regulator AP-1, a Fos-Jun heterodimer, in activity-dependent dendritic structure development. We combine genetic manipulation, imaging and quantitative dendritic architecture analysis in a Drosophila single neuron model, the individually identified motoneuron MN5. First, Dα7 nicotinic acetylcholine receptors (nAChRs) and AP-1 are required for normal MN5 dend…

Tips and turns of bacteriophytochrome photoactivation

2020

Phytochromes are ubiquitous photosensor proteins, which control the growth, reproduction and movement in plants, fungi and bacteria. Phytochromes switch between two photophysical states depending on the light conditions. In analogy to molecular machines, light absorption induces a series of structural changes that are transduced from the bilin chromophore, through the protein, and to the output domains. Recent progress towards understanding this structural mechanism of signal transduction has been manifold. We describe this progress with a focus on bacteriophytochromes. We describe the mechanism along three structural tiers, which are the chromophore-binding pocket, the photosensory module,…

Microvesicles shed by oligodendroglioma cells and rheumatoid synovial fibroblasts contain aggrecanase activity

2012

Membrane microvesicle shedding is an active process and occurs in viable cells with no signs of apoptosis or necrosis. We report here that microvesicles shed by oligodendroglioma cells contain an ‘aggrecanase’ activity, cleaving aggrecan at sites previously identified as targets for adamalysin metalloproteinases with disintegrin and thrombospondin domains (ADAMTSs). Degradation was inhibited by EDTA, the metalloproteinase inhibitor GM6001 and by tissue inhibitor of metalloproteinases (TIMP)-3, but not by TIMP-1 or TIMP-2. This inhibitor profile indicates that the shed microvesicles contain aggrecanolytic ADAMTS(s) or related TIMP-3-sensitive metalloproteinase(s). The oligodendroglioma cells…

Energy management of a thermally coupled fuel cell system and metal hydride tank

2019

International audience; Being produced from renewable energy, hydrogen is one of the most efficient energy carriers of the future. Using metal alloys, hydrogen can be stored and transported at a low cost, in a safe and effective manner. However, most metals react with hydrogen to form a compound called metal hydride (MH). This reaction is an exothermic process, and as a result releases heat. With sufficient heat supply, hydrogen can be released from the as-formed metal hydride. In this work, we propose an integrated power system of a proton exchange membrane fuel cell (PEMFC) together with a hydride tank designed for vehicle use. We investigate different aspects for developing metal hydride…

P23. Large demyelinating lesion of the pons as a cause of a locked-in syndrome in multiple sclerosis

2007

Experimental observations of topologically guided water waves within non-hexagonal structures

2020

International audience; We investigate symmetry-protected topological water waves within a strategically engineered square lattice system. Thus far, symmetry protected topological modes in hexagonal systems have primarily been studied in electromagnetism and acoustics, i.e., dispersionless media. Herein, we show experimentally how crucial geometrical properties of square structures allow for topological transport that is ordinarily forbidden within conventional hexagonal structures. We perform numerical simulations that take into account the inherent dispersion within water waves and devise a topological insulator that supports symmetry-protected transport along the domain walls. Our measur…

Influence of electromagnetic boundary conditions onto the onset of dynamo action in laboratory experiments

2009

We study the onset of dynamo action of the Riga and Karlsruhe experiments with the addition of an external wall, the electro-magnetic properties of which being different from those of the fluid in motion. We consider a wall of different thickness, conductivity and permeability. We also consider the case of a ferro-fluid in motion.

Induction mechanisms in a von Kármán swirling flows of liquid Gallium

2004

International audience; Using in situ magnetic field measurements, we study the induction mechanisms in a swirling flow of liquid Gallium generated inside a cylinder, in the gap between two coaxial rotating discs. The von Kármán flow generated in this manner has both helicity and differential rotation. Magnetic Reynolds numbers Rm up to 7 (based on the disc rim speed) are generated. We study the magnetic induction when an external field is applied successively along the axis, in the azimuthal direction or tranverse to the axis of rotation. In the first two cases, both the flow and the magnetic field are axisymmetric, and an effective mechanism of conversion from poloidal to toroidal field e…

Open questions about homogeneous fluid dynamos: The VKS experiment

2002

International audience; We consider several problems that arise in the context of homogeneous fluid dynamos such as the effect of turbulence on the dynamo threshold, the saturation level of the generated magnetic field above the threshold and its dynamics. We compare some of our predictions with the recent experimental results of the Karlsruhe and Riga experiments. Finally, we present the VKS experiment that we have designed to answer some of the remaining open questions. We study, in particular, the response of a turbulent flow to an external magnetic field.