Search results for "MEF"
showing 10 items of 96 documents
Etude comparative de deux méthodes de transformation et de deux milieux de culture chez Agrobacterium tumefaciens souche LBA4404
2013
Rapport de stage de master 1 BAP GEAPSI
Prédiction de cycle et stratégie de gestion énergétique basée sur une méthode d’optimisation globale
2017
International audience; La nécessité de prédire le cycle de conduite d’un automobiliste afin d’améliorer sa gestion énergétique et ainsi réduire la consommation de carburant a été démontré (Debert, Quigley). Dès lors, il serait aisé d’appliquer une méthode d’optimisation globale, telle que la Programmation Dynamique (DP en anglais), pour obtenir la stratégie optimale. Cette méthode est déjà utilisée dans de nombreuses applications (Sciarretta), mais au quotidien nombreux sont les facteurs environnants qui peuvent perturber les conditions de circulation (météo, travaux, accidents....) et impacter le cycle de conduite. Il est donc nécessaire d’élaborer une stratégie adaptative en temps réel. …
The Numerical Challenges in Multiphysical Modeling of Laser Welding with ALE
2018
International audience
Characterisation of mechanical behavior of a sub surface drilled polymer, under hertzian contact load
2013
This research concerns the understanding of the sub-surface behaviour of a component presenting controlled heterogeneities and subjected to a Hertzian type load. The purpose is to qualify the stress field in the subsurface. Practically, defects of cylindrical shape are located where the stress field is maximal in sub surface. The contact is sized to be able to observe, by photoelasticity, the stress field and to develop a numerical model to simulate the behaviour (static and dynamic) of the friction area. It is highlighted that the presence of a hole on the z axis of symmetry (x = 0) is the dominating factor. The characteristics which allow decreasing the shearing stress on the symmetry axi…
Plant hormones and Agrobacterium tumefaciens strain 82.139 induce efficient plant regeneration in the cardenolide-producing plant Digitalis minor
2002
Summary Shoot formation in explants of Digitalis minor was achieved, through axillary bud proliferation and adventitious bud differentiation, by varying the amount and source of plant hormones. Shoot regeneration was also obtained after infection of D. minor with the wild-type Agrobacterium tumefaciens strain 82.139, which induced shooty tumours. These shoots were not transgenic, as revealed by nopaline assays and the use of a C58pMP90/T139GUS-INT strain harbouring the intron inactivated gusA gene. Plants were easily rooted and transplanted into the greenhouse. Shoot cultures of D. minor accumulated up to 226 μg cardenolides per g dry mass when cultured on agar-solidified medium. Cardenolid…
Neutralizing antibodies against rotavirus produced in transgenically labelled purple tomatoes
2011
Edible fruits are inexpensive biofactories for human health-promoting molecules that can be ingested as crude extracts or partially purified formulations. We show here the production of a model human antibody for passive protection against the enteric pathogen rotavirus in transgenically labelled tomato fruits. Transgenic tomato plants expressing a recombinant human immunoglobulin A (hIgA_2A1) selected against the VP8* peptide of rotavirus SA11 strain were obtained. The amount of hIgA_2A1 protein reached 3.6 ± 0.8% of the total soluble protein in the fruit of the transformed plants. Minimally processed fruit-derived products suitable for oral intake showed anti-VP8* binding activity and str…
Estimation of the density of the protocatechuate-degrading bacterial community in soil by real-time PCR
2008
Summary The β-ketoadipate pathway is the major route for degradation of aromatic compounds by various soil microorganisms. Protocatechuate 3,4-dioxygenase, a key enzyme of this pathway and which is encoded by pcaGH genes, catalyses the ring cleavage of protocatechuate. Microorganisms harbouring pcaGH genes are widely distributed in the environment but little is known about their relative abundance within the total microflora. Hence, this paper reports the development of a real-time PCR assay to quantify the bacterial pcaH sequence that encodes the β sub-unit of the protocatechuate 3,4-dioxygenase. This real-time PCR assay was linear over seven orders of magnitude with a calculated efficienc…
EZH2 mutations are frequent and represent an early event in follicular lymphoma
2013
Gain of function mutations in the H3K27 methyltransferase EZH2 represent a promising therapeutic target in germinal center lymphomas. In this study, we assessed the frequency and distribution of EZH2 mutations in a large cohort of patients with follicular lymphoma (FL) (n = 366) and performed a longitudinal analysis of mutation during the disease progression from FL to transformed FL (tFL) (n = 33). Mutations were detected at 3 recurrent mutation hot spots (Y646, A682, and A692) in 27% of FL cases with variant allele frequencies (VAF) ranging from 2% to 61%. By comparing VAF of EZH2 with other mutation targets (CREBBP, MLL2, TNFRSF14, and MEF2B), we were able to distinguish patients harbori…
The importance of fracture-healing on the deformation of fluid-filled layered systems
2014
International audience; Understanding the fracturingehealingerefracturing cycle is a fundamental part of studying the deformation dynamics and the permeability evolution of rock systems. Previous studies, however, have not examined the influence of healing i.e. fracture-closure through vein formation and the mechanical properties of the " healed " fractures (veins) on the rock deformation. We present results from a two-dimensional coupled hydro-mechanical model which simulates large time and spatial scale dynamic fracturing and healing of a porous medium under the influence of gravity, tectonic stretching and elevated fluid pressures. Our results show that healing decreases the local porosi…
A web-based collection of genotype-phenotype associations in hereditary recurrent fevers from the Eurofever registry
2017
PubMed ID: 29047407