Search results for "MEG"

Micromorphological approach to the systematics of Mediterranean Isoëtes species (Isoëtaceae, Lycopodiophyta): analysis of the megaspore surface

2012

Megaspore ornamentation is one of the few morphological characters used in the taxonomy of the genus Isoëtes. In the present work, we test the application of this character for distinguishing some Isoëtes species occurring in the Mediterranean: Isoëtes sicula, which according to some authors should be included as a variety in I. histrix, and the recently described I. todaroana, whose affinity with other taxa is currently unknown. Two additional species (I. duriei, I. velata) were also included in the analysis. The megaspores were studied using scanning electron microscopy combined with energy-dispersive X-ray spectroscopy and chemical treatment. In all species, the megaspore surface is cove…

An update on the management and prevention of cytomegalovirus infection following allogeneic hematopoietic stem cell transplantation

2015

ABSTRACT  A significant progress has been made in deciphering critical aspects of the biology and immunology of CMV infection in the allogeneic stem cell transplantation setting. Genetic traits predisposing to active CMV infection and CMV end-organ disease have begun to be delineated. Reliable molecular assays for CMV DNA load quantitation in body fluids have been developed. Elucidation of immune mechanisms affording control of CMV infection will help to improve the management of active CMV infection. Finally, the advent of new CMV-specific antivirals and promising vaccine prototypes as well as the development of fine procedures for large-scale ex vivo generation of functional CMV-specific…

Large bulk Micromegas detectors for TPC applications

2009

A large volume TPC will be used in the near future in a variety of experiments including T2K. The bulk Micromegas detector for this TPC is built using a novel production technique particularly suited for compact, thin and robust low mass detectors. The capability to pave a large Surface with a simple mounting Solution and small dead space is of particular interest for these applications. We have built several large bulk Micromegas detectors (36 x 34 cm(2)) and we have tested one in the former HARP field cage with a magnetic field. Prototypes cards of the T2K front end electronics, based on the AFTER ASIC chip, have been used in this TPC test for the first time. Cosmic ray data have been acq…

Sustained activation of mTOR pathway in embryonic neural stem cells leads to development of tuberous sclerosis complex-associated lesions

2011

SummaryTuberous Sclerosis Complex (TSC) is a multisystem genetic disorder characterized by hamartomatous neurological lesions that exhibit abnormal cell proliferation and differentiation. Hyperactivation of mTOR pathway by mutations in either the Tsc1 or Tsc2 gene underlies TSC pathogenesis, but involvement of specific neural cell populations in the formation of TSC-associated neurological lesions remains unclear. We deleted Tsc1 in Emx1-expressing embryonic telencephalic neural stem cells (NSCs) and found that mutant mice faithfully recapitulated TSC neuropathological lesions, such as cortical lamination defects and subependymal nodules (SENs). These alterations were caused by enhanced gen…

Theognis of Megara and the Divine Creating Power in the Framework of Semiotic Textology: An Application of János Sándor Petöfi’s Theory to Archaic Gr…

2012

This paper is a demonstration of an application of Semiotic Textology to a limited case study. The main aspects of Semiotic Textology, the theory elaborated by Petöfi, are presented; secondly the linguistic aspects of the interpretation of lines 133-134 of the Theognis of Megara's poem, analysed in the framework of said theory, are presented. All the relevant syntactic, semantic, pragmatic information involved in text processing have been considered. Through fixed steps, it is shown that text processing is not exclusively a grammatical activity, because within a theoretical interpretation an Interpreter needs a number of contextual hypotheses, in order to understand the author's ontology. ©…

Oral valganciclovir for coloni c dilatation in ulcerative colitis associated with Human cytomegalovirus infection

2009

Immune Evasion Proteins Enhance Cytomegalovirus Latency in the Lungs

2009

ABSTRACT CD8 T cells control cytomegalovirus (CMV) infection in bone marrow transplantation recipients and persist in latently infected lungs as effector memory cells for continuous sensing of reactivated viral gene expression. Here we have addressed the question of whether viral immunoevasins, glycoproteins that specifically interfere with antigen presentation to CD8 T cells, have an impact on viral latency in the murine model. The data show that deletion of immunoevasin genes in murine CMV accelerates the clearance of productive infection during hematopoietic reconstitution and leads to a reduced latent viral genome load, reduced latency-associated viral transcription, and a lower inciden…

CD8 T Cells Control Cytomegalovirus Latency by Epitope-Specific Sensing of Transcriptional Reactivation

2006

ABSTRACT During murine cytomegalovirus (mCMV) latency in the lungs, most of the viral genomes are transcriptionally silent at the major immediate-early locus, but rare and stochastic episodes of desilencing lead to the expression of IE1 transcripts. This low-frequency but perpetual expression is accompanied by an activation of lung-resident effector-memory CD8 T cells specific for the antigenic peptide 168-YPHFMPTNL-176, which is derivedfrom the IE1 protein. These molecular and immunological findings were combined in the “silencing/desilencing and immune sensing hypothesis” of cytomegalovirus latency and reactivation. This hypothesis proposes that IE1 gene expression proceeds to cell surfac…

Transactivation of cellular genes involved in nucleotide metabolism by the regulatory IE1 protein of murine cytomegalovirus is not critical for viral…

2008

ABSTRACT Despite its high coding capacity, murine CMV (mCMV) does not encode functional enzymes for nucleotide biosynthesis. It thus depends on cellular enzymes, such as ribonucleotide reductase (RNR) and thymidylate synthase (TS), to be supplied with deoxynucleoside triphosphates (dNTPs) for its DNA replication. Viral transactivation of these cellular genes in quiescent cells of host tissues is therefore a parameter of viral fitness relevant to pathogenicity. Previous work has shown that the IE1, but not the IE3, protein of mCMV transactivates RNR and TS gene promoters and has revealed an in vivo attenuation of the mutant virus mCMV-ΔIE1. It was attractive to propose the hypothesis that la…

Transkriptoma analīze akromegālijas pacientiem ar un bez medikamentozās terapijas

2019

Darba mērķis bija veikt somatotrofas hipofīzes adenomas audu transkriptoma analīzi pacientiem, kuri ir vai nav lietojuši medikamentozo terapiju pirms operācijas, analizējot SST/DA analogu izraisītās izmaiņas transkriptomā. Lai to realizētu tika izdalīts totālais RNS no pacientu somatotrofās hipofīzes adenomas audiem un sagatavotas transkriptoma bibliotēkas. Pēc tam iegūtie dati tika analizēti, izmantojot Galaxy platformu un programmu R un R Studio. Pēc datu analīzes tika iegūti 22 statistiski būtiski DEGs un no tiem viens būtiski iesaistīts signālceļš, kā arī intensitātes grafikā tika novērotas divas izteiktas gēnu grupas, kas iedalijās starp pacientiem atkarībā no medikamentozās terapijas.