Search results for "MEP"

showing 10 items of 209 documents

A novel haemoprotein induced by isosafrole pretreatment in the rat

1978

Abstract Sodium dodecyl sulphate-polyacrylamide gel electrophoresis has been used to demonstrate that pretreatment of rats with isosafrole results in the formation of a novel species of cytochrome P-450 (mol. wt. 54,000) quite distinct from that induced by phenobarbitone pretreatment (mol. wt. 50,000) or 3-methylcholanthrene (mol. wt. 58,000).

MaleHemeproteinCytochromeSodiumBiophysicschemistry.chemical_elementDioxolesBiochemistrychemistry.chemical_compoundCytochrome P-450 Enzyme SystemIsomerismSafroleMoleAnimalsMolecular BiologyGel electrophoresisChromatographybiologyChemistrySpectrum AnalysisCell BiologyRatsMolecular WeightBiochemistryIsosafroleEnzyme InductionPhenobarbitalMicrosomes Liverbiology.proteinApoproteinsMethylcholanthreneBiochemical and Biophysical Research Communications
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A model-based meta-analysis of sofosbuvir-based treatments in chronic hepatitis C patients.

2016

The objective of this study was to compare the efficacy of sofosbuvir-based treatments in patients with chronic hepatitis C virus (HCV) infection using a model-based meta-analysis (MBMA). A bibliographic search was performed to identify clinical trials involving sofosbuvir as a unique direct-acting antiviral (DAA) agent or together with daclatasvir, ledipasvir or simeprevir for the treatment of diagnosed HCV infection. The time course of the virological response (VR) was modelled to estimate the effect of treatment and the influence of population characteristics on the longitudinal efficacy profile. The model was validated and simulations of 10 different treatment schedules were performed. …

MaleMicrobiology (medical)OncologyLedipasvirSimeprevirmedicine.medical_specialtyPyrrolidinesDaclatasvirSofosbuvirHepatitis C virusPopulationHepacivirusPharmacologymedicine.disease_causeAntiviral Agents03 medical and health scienceschemistry.chemical_compound0302 clinical medicineSimeprevirInternal medicinemedicineHepatitis C virus Meta-analysis Modelling Simulation SofosbuvirHumansPharmacology (medical)030212 general & internal medicineeducationFluoreneseducation.field_of_studybusiness.industryImidazolesValineGeneral MedicineHepatitis C ChronicModels TheoreticalClinical trialInfectious DiseaseschemistryMeta-analysisBenzimidazolesDrug Therapy CombinationFemale030211 gastroenterology & hepatologyCarbamatesSofosbuvirbusinessmedicine.drug
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Mepolizumab effectiveness on small airway obstruction, corticosteroid sparing and maintenance therapy step-down in real life

2020

Background: Mepolizumab (MEP) has been recently introduced to treat severe eosinophilic asthma. Trials have demonstrated a significant effectiveness in this asthma phenotype. We evaluated MEP efficacy on lung function, symptoms, asthma exacerbations, biologic markers, steroid dependence and controller treatment level in real-life. Methods: We retrospectively analyzed 134 severe asthmatics (61 males; mean age 58.3 ± 11; mean FEV1%:72 ± 21), treated with MEP for at least 6 months (mean duration:10.9 ± 3.7 months). Results: FEV1% improved significantly after MEP. Mean FEF25-75 also increased from 37.4 ± 25.4% to 47.2 ± 27.2% (p < 0.0001). Mean baseline blood eosinophil level was 712 ± 731/μ…

MaleOmalizumabFEF(25-75)step-down0302 clinical medicineMaintenance therapyAdrenal Cortex HormonesSettore MED/10Pharmacology (medical)Anti-Asthmatic Agents030212 general & internal medicinesmall airwaysAsthma exacerbationsmepolizumabMiddle AgedAnesthesiaFEF25-75CorticosteroidDrug Therapy CombinationFemalemedicine.drugPulmonary and Respiratory Medicinesevere asthmamedicine.drug_classMepolizumab Severe asthma Real-life Small airways FEF25-75 Oral corticosteroids Step-down EffectiveneseffectivenessSettore MED/10 - Malattie Dell'Apparato Respiratoriooral corticosteroidsAntibodies Monoclonal HumanizedNO03 medical and health sciencesEffectiveness FEF 25-75 Mepolizumab Oral corticosteroids Real-life Severe asthma Small airways Step-downmedicineHumansIn real lifeFEFreal-lifeAgedRetrospective StudiesBiologic markerbusiness.industryEffectivenesBiochemistry (medical)Effectiveness; FEF; 25-75; Mepolizumab; Oral corticosteroids; Real-life; Severe asthma; Small airways; Step-downAirway obstructionmedicine.diseaseAsthmaBlood Cell Countrespiratory tract diseasesAirway ObstructionEosinophils030228 respiratory systemeffectiveness; FEF(25-75); mepolizumab; oral corticosteroids; real-life; severe asthma; small airways; step-down25-75businessMepolizumab
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Five-Year Follow-up of Patients Receiving Imatinib for Chronic Myeloid Leukemia

2006

The cause of chronic myeloid leukemia (CML) is a constitutively active BCR-ABL tyrosine kinase. Imatinib inhibits this kinase, and in a short-term study was superior to interferon alfa plus cytarabine for newly diagnosed CML in the chronic phase. For 5 years, we followed patients with CML who received imatinib as initial therapy.We randomly assigned 553 patients to receive imatinib and 553 to receive interferon alfa plus cytarabine and then evaluated them for overall and event-free survival; progression to accelerated-phase CML or blast crisis; hematologic, cytogenetic, and molecular responses; and adverse events.The median follow-up was 60 months. Kaplan-Meier estimates of cumulative best …

MaleOncologymedicine.medical_specialtyFusion Proteins bcr-ablAntineoplastic AgentsKaplan-Meier EstimateChronic phase chronic myelogenous leukemiaDisease-Free SurvivalPiperazineschemistry.chemical_compoundLeukemia Myelogenous Chronic BCR-ABL Positivehemic and lymphatic diseasesInternal medicineAntineoplastic Combined Chemotherapy ProtocolsOmacetaxine mepesuccinatemedicineHumansneoplasmsbusiness.industryPonatinibCytarabineInterferon-alphaMyeloid leukemiaImatinibGeneral MedicineProtein-Tyrosine KinasesSurvival AnalysisSurvival RateDasatinibPyrimidinesTreatment OutcomeImatinib mesylatechemistryNilotinibBenzamidesImmunologyImatinib MesylateFemalebusinessFollow-Up Studiesmedicine.drugNew England Journal of Medicine
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Inflammatory Pain Promotes Increased Opioid Self-Administration: Role of Dysregulated Ventral Tegmental Area μ Opioid Receptors

2015

Pain management in opioid abusers engenders ethical and practical difficulties for clinicians, often resulting in pain mismanagement. Although chronic opioid administration may alter pain states, the presence of pain itself may alter the propensity to self-administer opioids, and previous history of drug abuse comorbid with chronic pain promotes higher rates of opioid misuse. Here, we tested the hypothesis that inflammatory pain leads to increased heroin self-administration resulting from altered mu opioid receptor (MOR) regulation of mesolimbic dopamine (DA) transmission. To this end, the complete Freund's adjuvant (CFA) model of inflammation was used to assess the neurochemical and functi…

MalePain ThresholdSucroseReceptors Opioid muAction PotentialsPainMesolimbic pathwayPharmacologyHeroinRats Sprague-DawleyQuinoxalinesThreshold of painmental disordersmedicineAnimalsInflammationNeuronsGeneral NeuroscienceVentral Tegmental AreaChronic painGlycine AgentsArticlesStrychnineEnkephalin Ala(2)-MePhe(4)-Gly(5)-medicine.diseaseRatsVentral tegmental areaAnalgesics OpioidHeroinDisease Models Animalmedicine.anatomical_structureOpioidInhibitory Postsynaptic PotentialsHyperalgesiaHyperalgesiaConditioning Operantμ-opioid receptormedicine.symptomPsychologyExcitatory Amino Acid Antagonistsmedicine.drug
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Imaging of Orthotopic Glioblastoma Xenografts in Mice Using a Clinical CT Scanner: Comparison with Micro-CT and Histology

2016

Purpose There is an increasing need for small animal in vivo imaging in murine orthotopic glioma models. Because dedicated small animal scanners are not available ubiquitously, the applicability of a clinical CT scanner for visualization and measurement of intracerebrally growing glioma xenografts in living mice was validated. Materials and Methods 2.5x106 U87MG cells were orthotopically implanted in NOD/SCID/ᵞc-/- mice (n = 9). Mice underwent contrast-enhanced (300 μl Iomeprol i.v.) imaging using a micro-CT (80 kV, 75 μAs, 360° rotation, 1,000 projections, scan time 33 s, resolution 40 x 40 x 53 μm) and a clinical CT scanner (4-row multislice detector; 120 kV, 150 mAs, slice thickness 0.5 …

MalePathologyCancer Treatmentlcsh:MedicineContrast MediaMice SCIDSignal-To-Noise RatioDiagnostic Radiology030218 nuclear medicine & medical imagingchemistry.chemical_compound0302 clinical medicineMice Inbred NODMedicine and Health Scienceslcsh:ScienceSmall AnimalsTomographyNeurological TumorsMice KnockoutMultidisciplinarymedicine.diagnostic_testBrain NeoplasmsRadiology and ImagingBrainGliomaMagnetic Resonance ImagingIn Vivo ImagingOncologyNeurology030220 oncology & carcinogenesisFemaleAnatomyPreclinical imagingResearch ArticleInterleukin Receptor Common gamma Subunitmedicine.medical_specialtyHistologyImaging TechniquesAnimal TypesTransplantation HeterologousIomeprolBrain tumorNeuroimagingResearch and Analysis Methods03 medical and health sciencesDiagnostic MedicineCell Line TumorGliomamedicineAnimalsHumansMultislicebusiness.industrylcsh:ROrganismsBiology and Life SciencesCancers and NeoplasmsReproducibility of ResultsMagnetic resonance imagingX-Ray Microtomographymedicine.diseaseComputed Axial TomographyIopamidolTransplantationSignal-to-noise ratio (imaging)chemistrylcsh:QGlioblastomabusinessNuclear medicineZoologyNeurosciencePLOS ONE
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Comparable Helicobacter pylori eradication rates obtained with 4- and 7-day rabeprazole-based triple therapy: a preliminary study

2003

Abstract Background. Rabeprazole is a new proton pump inhibitor, which has been reported to induce a faster acid suppression than other drugs of the same category. This might be useful to reduce the duration of anti-Helicobacter therapies. Aims. The aim of this study was to assess whether there is the possibility of shortening a rabeprazole-based triple therapy from 7 to 4 days without compromising its efficacy in the eradication of Helicobacter pylori infection. Patients. A total of 128 consecutive dyspeptic patients with H. pylori infection were recruited for this controlled, randomized, open and parallel-group trial comparing the efficacy of two durations of the same rabeprazole-based tr…

MalePeptic Ulcermedicine.medical_specialtymedicine.drug_classUrea breath testRabeprazoleProton-pump inhibitorPilot ProjectsGastroenterology2-PyridinylmethylsulfinylbenzimidazolesDrug Administration ScheduleHelicobacter InfectionsAnti-Infective AgentsClarithromycinMetronidazoleClarithromycinInternal medicinemedicineHumansProspective StudiesDyspepsiaAdverse effectHelicobacter pyloriHepatologybiologymedicine.diagnostic_testbusiness.industryGastroenterologyProton Pump InhibitorsMiddle AgedHelicobacter pyloriAnti-Ulcer Agentsbiology.organism_classificationAnti-Bacterial AgentsMetronidazoleRegimenTreatment OutcomeRabeprazoleBenzimidazolesDrug Therapy CombinationFemalebusinessOmeprazolemedicine.drug
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A novel LC–MS/MS analytical method for detection of articaine and mepivacaine in blood and its application to a preliminary pharmacokinetic study

2020

Local anaesthetics (LAs) are commonly used in surgery, especially in dentistry. They cause a transitory inhibition of nerve signal due to the blockade of the voltage-gated sodium channels. LAs are administrated alone or with vasoconstriction agents, such as adrenaline. Toxicity of LAs is associated to neurological and cardiovascular alterations. Tachycardia, arrhythmia, tremors, tonic-clonic seizure and respiratory depression (at high doses) are the main symptoms of intoxication by LAs. Lidocaine, articaine and mepivacaine are among the most used anaesthetics. This study aimed to fully validated a new method for the simultaneous detection of articaine and mepivacaine in whole blood. Sample …

MaleTachycardiaLidocaineLiquid-Liquid ExtractionClinical BiochemistryMepivacainePharmaceutical ScienceCarticaineArticaine01 natural sciencesAnalytical ChemistryPharmacokineticsSettore MED/43 - Medicina LegaleLC–MS/MSTandem Mass SpectrometryArticaine Mepivacaine Local anaesthetics LC–MS/MS BloodbloodDrug DiscoverymedicineHumansAnesthetics LocalSpectroscopyarticaine; blood; LC–MS/MS; local anaesthetics; mepivacaineWhole blood010405 organic chemistryChemistry010401 analytical chemistrySelected reaction monitoringReproducibility of Results0104 chemical sciencesarticaineAnesthesiaToxicityFemalelocal anaestheticsmedicine.symptomChromatography Liquidmedicine.drugmepivacaine
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Gene Transcription Alterations Associated with Decrease of Ethanol Intake Induced by Naltrexone in the Brain of Wistar Rats

2006

Preclinical and clinical studies suggest that the administration of the opioid antagonist naltrexone decreases the intake of ethanol. However, the neuroplastic adaptations in the brain associated to reduction of ethanol consumption remains to be elucidated. The aim of the study was to identify gene transcription alterations underlying the attenuation of voluntary ethanol intake by administration of naltrexone in rats. Increasing doses of naltrexone (0.7 mg/kg, 4 days and 1.4 mg/kg/day, 4 days) to rats with acquired high preferring ethanol consumption (>3.5 g of ethanol/kg/day) decreased voluntary ethanol intake (50%). Voluntary ethanol consumption altered mu-opioid receptor function in the …

Malemedicine.medical_specialtyAlcohol DrinkingTranscription Geneticmedicine.drug_classNarcotic AntagonistsNucleus accumbensPharmacologyNaltrexoneInternal medicineImage Processing Computer-AssistedmedicineAnimalsRats WistarOpioid peptideIn Situ HybridizationBrain ChemistryPharmacologyEthanolTyrosine hydroxylaseChemistryOlfactory tubercleCentral Nervous System DepressantsEnkephalin Ala(2)-MePhe(4)-Gly(5)-NaltrexoneRatsAnalgesics OpioidVentral tegmental areaPsychiatry and Mental healthmedicine.anatomical_structureEndocrinologynervous systemGuanosine 5'-O-(3-Thiotriphosphate)HypothalamusAutoradiographyOpioid antagonistmedicine.drugNeuropsychopharmacology
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The effects of LSD in the guinea-pig ileum

1985

The effects of lysergic acid diethylamide (LSD) on acetylcholine release and on smooth muscle tone were studied in the myenteric plexus-longitudinal muscle preparation of the guinea pig. LSD (0.01-10 microM) depressed in a concentration-dependent manner the electrically-evoked [3H]-acetylcholine outflow from strips preincubated with [3H]-choline. The maximal effect was a 45% inhibition by 1 microM LSD. The spontaneous outflow was not affected. Metitepine competitively antagonized (pA2 8.0) the LSD-induced reduction of the evoked outflow. Tolazoline and mepyramine did not affect the inhibitory action of LSD. The contractions in response to electrical stimulation were enhanced by 34% in the p…

Malemedicine.medical_specialtyKetanserinGuinea PigsMepyramineMyenteric PlexusStimulationchemistry.chemical_compoundMuscle toneHistamine receptorIleumInternal medicinemedicineAnimalsReceptors Histamine H15-HT receptorPharmacologyMuscle SmoothGeneral MedicineAcetylcholineLysergic Acid DiethylamideEndocrinologymedicine.anatomical_structurechemistryMuscle TonusReceptors SerotoninMetitepineFemaleAcetylcholineMuscle Contractionmedicine.drugNaunyn-Schmiedeberg's Archives of Pharmacology
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