Search results for "MICROBIOLOGICAL"
showing 10 items of 83 documents
Preliminary evaluation of biopolymers production by mixed microbial culture from citrus wastewater in a MBR system using respirometric techniques
2021
Abstract This preliminary study was aimed at evaluating the feasibility to produce biopolymers (BP) from citrus wastewater by mixed microbial culture in an anaerobic/aerobic membrane bioreactor (A/O-MBR). The activated sludge of the A/O-MBR was successfully enriched in microorganisms having a good capacity in producing intracellular biopolymers. The production of BP was found to be about 0.55 mgCOD mgCOD−1 using pure acetate at a concentration of 1000 mgCOD L−1. When using fermented wastewater, the conversion of acetate into BP product was 0.56 mgCOD mgCOD−1 in the test performed with C/N equal to 1000:1, whereas it was only 0.12 mgCOD mgCOD−1 in the test with C/N of 100:5. The results achi…
The Effectiveness of 0.6% Povidone Iodine Eye Drops in Reducing the Conjunctival Bacterial Load and Needle Contamination in Patients Undergoing Anti-…
2019
The study purpose was to assess the efficacy of a preservative-free 0.6% povidone iodine eye drops as perioperative prophylactic treatment for reducing conjunctival bacterial load and the rate of needle contamination in patients undergoing intravitreal anti-vascular endothelial growth factor injection. Enrolled patients were randomized to either the study group (0.6% povidone iodine, three day-prophylactic treatment before the injection) or to the control group (placebo, three day-prophylactic treatment). Conjunctival swabs were obtained before and after the prophylactic treatment in both groups. Intravitreal injections were performed in a sterile fashion. The injection needle and a control…
Diagnóstico microbiológico de las infecciones gastrointestinales
2009
Resumen Las infecciones agudas del tracto gastrointestinal figuran entre las enfermedades infecciosas más frecuentes. En esta revisión se examinan diversas técnicas para diagnosticar las gastroenteritis que ocasionan bacterias, virus y parásitos. El coprocultivo es el método de elección para el diagnóstico de las infecciones bacterianas intestinales, aunque las infecciones por Clostridium difficile se pueden diagnosticar mediante la detección de las toxinas A y B en las heces y las infecciones por Escherichia coli diarreagénicas se pueden diagnosticar mediante la detección por reacción en cadena de la polimerasa de factores de virulencia específicos de los diversos enteropatotipos. Las técn…
Epidemiological cutoff values for fluconazole, itraconazole, posaconazole, and voriconazole for six Candida species as determined by the colorimetric…
2013
ABSTRACT In the absence of clinical breakpoints (CBP), epidemiological cutoff values (ECVs) are useful to separate wild-type (WT) isolates (without mechanisms of resistance) from non-WT isolates (those that can harbor some resistance mechanisms), which is the goal of susceptibility tests. Sensititre YeastOne (SYO) is a widely used method to determine susceptibility of Candida spp. to antifungal agents. The CLSI CBP have been established, but not for the SYO method. The ECVs for four azoles, obtained using MIC distributions determined by the SYO method, were calculated via five methods (three statistical methods and based on the MIC 50 and modal MIC). Respectively, the median ECVs (in mg/lit…
History and services of culture collections.
2003
Microbial culture collections aim at collecting, maintaining and distributing microbial strains among microbiologists, and are considered to be a means to preserve microbial diversity ex situ. This article reviews the early history of culture collections, which were first set up in the late nineteenth century, with the introduction of pure culture techniques. It also describes the international connections between culture collections, which led to the foundation of the World Federation for Culture Collections (WFCC) and the International Congress of Culture Collections (ICCC). An overview of the Spanish Type Culture Collection (CECT) is included, as well as a description of the services tha…
Inter-laboratory evaluation of the ISO standard 11063 "Soil quality - Method to directly extract DNA from soil samples"
2011
International audience; Extracting DNA directly from micro-organisms living in soil is a crucial step for the molecular analysis of soil microbial communities. However, the use of a plethora of different soil DNA extraction protocols, each with its own bias, makes accurate data comparison difficult. To overcome this problem, a method for soil DNA extraction was proposed to the International Organization for Standardization (ISO) in 2006. This method was evaluated by 13 independent European laboratories actively participating in national and international ring tests. The reproducibility of the standardized method for molecular analyses was evaluated by comparing the amount of DNA extracted, …
Phylogenetic analysis of environmental Legionella pneumophila isolates from an endemic area (Alcoy, Spain).
2015
Environmental surveillance of Legionella pneumophila is a key component of the control measures established in urban settlements to ensure water safety and quality, with the aim of minimizing and limiting opportunistic infections in humans. In this work, we present results on the detection and genetic characterization of these bacteria in the outbreak-recurrent region of Alcoy (Comunidad Valenciana, Spain) using water and biofilm samples. We were particularly interested in studying the presence and distribution of L. pneumophila in the absence of outbreak or sporadic cases of legionellosis and in comparing the efficacy of culturing from water samples with a biofilm-based detection procedure…
Optimization of the preanalytical steps of matrix-assisted laser desorption ionization-time of flight mass spectrometry identification provides a fle…
2012
ABSTRACT We report here that modifications of the preanalytical steps of matrix-assisted laser desorption ionization–time of flight mass spectrometry (MALDI-TOF MS) identification of yeasts, with regard to the original protocol provided by the manufacturers, appear to be efficient for the reliable routine identification of clinical yeast isolates in medical laboratories. Indeed, when one colony was sampled instead of five and the protein extraction protocol was modified, the performance of MALDI-TOF MS was superior to that of the API ID 32C method (discrepancies were confirmed by using molecular identification), allowing the correct identification of 94% of the 335 clinical isolates prospec…
Molecular Diagnosis of Toxoplasmosis in Immunocompromised Patients: a 3-Year Multicenter Retrospective Study
2015
ABSTRACT Toxoplasmosis is a life-threatening infection in immunocompromised patients (ICPs). The definitive diagnosis relies on parasite DNA detection, but little is known about the incidence and burden of disease in HIV-negative patients. A 3-year retrospective study was conducted in 15 reference laboratories from the network of the French National Reference Center for Toxoplasmosis, in order to record the frequency of Toxoplasma gondii DNA detection in ICPs and to review the molecular methods used for diagnosis and the prevention measures implemented in transplant patients. During the study period, of 31,640 PCRs performed on samples from ICPs, 610 were positive (323 patients). Blood ( n …
Comparison of Delivery Methods in Phage Therapy against Flavobacterium columnare Infections in Rainbow Trout
2021
Viruses of bacteria, bacteriophages, specifically infect their bacterial hosts with minimal effects on the surrounding microbiota. They have the potential to be used in the prevention and treatment of bacterial infections, including in the field of food production. In aquaculture settings, disease-causing bacteria are often transmitted through the water body, providing several applications for phage-based targeting of pathogens, in the rearing environment, and in the fish. We tested delivery of phages by different methods (via baths, in phage-coated material, and via oral delivery in feed) to prevent and treat Flavobacterium columnare infections in rainbow trout fry using three phages (FCOV…