Search results for "MLP"
showing 10 items of 22 documents
Tetrazīna fragmentu saturošu linkeru sintēze proteīnu modificēšanai
2022
Maģistra darbā ir veikta jaunu, alifātisku un PEG fragmentus saturošu, tetrazīna linkeru sintēze proteīnu biokonjugācijai. Darba ietvaros iegūti un izolēti 3 linkeri, kas paredzēti neselektīvai Lys aminoskābju atlikumu modificēšanai, un arī iegūti vēl 3 linkeri, kas paredzēti reģiospecifiskai proteīna N-gala modificēšanai. Darba ietvaros arī izolēts viens linkeris, kas paredzēts His modificēšanai. Visi sintezētie linkeri tika testēti modificējot Borrelia Burgdorferi MlpC lipoproteīnu, kā arī veikta paraugu MALDI-TOF MS analīze, kurā no septiņiem linkeriem, seši ir uzrādījuši konjugāta veidošanos ar MlpC.
Region of interest detection using MLP
2014
A novel technique to detect regions of interest in a time series as deviation from the characteristic behavior is proposed. The deterministic form of a signal is obtained using a reliably trained MLP neural network with detailed complexity management and cross-validation based generalization assurance. The proposed technique is demonstrated with simulated and real data. peerReviewed
Extracellular Vesicles From Liver Progenitor Cells Downregulates Fibroblast Metabolic Activity and Increase the Expression of Immune-Response Related…
2021
Extracellular vesicles (EVs) mediate cell-to-cell crosstalk whose content can induce changes in acceptor cells and their microenvironment. MLP29 cells are mouse liver progenitor cells that release EVs loaded with signaling cues that could affect cell fate. In the current work, we incubated 3T3-L1 mouse fibroblasts with MLP29-derived EVs, and then analyzed changes by proteomics and transcriptomics. Results showed a general downregulation of protein and transcript expression related to proliferative and metabolic routes dependent on TGF-beta. We also observed an increase in the ERBB2 interacting protein (ERBIN) and Cxcl2, together with an induction of ribosome biogenesis and interferon-relate…
Analisi di delezioni esoniche del gene PAH in pazienti affetti da iperfenilalaninemia
2009
Neuroblastoma after Childhood: Prognostic Relevance of Segmental Chromosome Aberrations, ATRX Protein Status, and Immune Cell Infiltration
2014
AbstractNeuroblastoma (NB) is a common malignancy in children but rarely occurs during adolescence or adulthood. This subgroup is characterized by an indolent disease course, almost uniformly fatal, yet little is known about the biologic characteristics. The aim of this study was to identify differential features regarding DNA copy number alterations, α-thalassemia/mental retardation syndrome X-linked (ATRX) protein expression, and the presence of tumor-associated inflammatory cells. Thirty-one NB patients older than 10 years who were included in the Spanish NB Registry were considered for the current study; seven young and middle-aged adult patients (range 18-60 years) formed part of the c…
Mutation analysis of the SPG4 gene in Italian patients with pure and complicated forms of spastic paraplegia
2010
Mutations in the SPG4 gene are the most common causes of hereditary spastic paraplegia (HSP) accounting for up to 40% of autosomal dominant (AD) forms and 12-18% of sporadic cases. The phenotype associated with HSP due to mutations in the SPG4 gene tends to be pure. There is increasing evidence, however, of patients with complicated forms of spastic paraplegia in which SPG4 mutations were identified. A cohort of 38 unrelated Italian patients with spastic paraplegia, of which 24 had a clear dominant inheritance and 14 were apparently sporadic, were screened for mutations in the SPG4 gene.We identified 11 different mutations, six of which were novel (p.Glu143GlyfsX8, p.Tyr415X, p.Asp548Asn, c…
Mobile Edge Computing: Architetture ed Analisi della Live Migration
2020
Con l'ormai prossima rete mobile 5G entreranno a far parte della nostra quotidianità nuovi servizi applicativi, mai prima possibili, grazie all'avvicinamento di risorse di calcolo e di memoria nei pressi dell'utente in mobilità. Un’architettura abilitante i futuri servizi è quella di Mobile Edge Computing (MEC) in cui cloud di capacità inferiori rispetto a quelli presenti nella core della rete sono dislocati nei pressi della stazioni radio e metteranno a disposizione risorse di calcolo tali da permettere, tramite la tecnica di offloading, la fruizione di servizi quali realtà aumentata, gaming online, contenuti streaming ad alta risoluzione ed operazioni di data analytics. Ogni nuovo paradig…
Analisi MLPA del gene CREB-binding protein (CREBBP) in un paziente con la sindrome di Rubinstein Taybi
2012
La sindrome di Rubinstein-Taybi è una rara malattia congenita autosomica dominante caratterizzata da ritardo della crescita postnatale, ritardo dello sviluppo psicomotorio, anomalie scheletriche, peculiare morfologia facciale ed un aumento del rischio oncogeno. La prevalenza alla nascita è 1 su 125.000 nati vivi. La malattia può essere associata a mutazioni nel gene che codifica per la proteina CREB-binding localizzato nella regione cromosomica 16p13.3. Recenti studi hanno dimostrato che pazienti con quoziente intellettivo basso e tratti autistici possono avere grandi delezioni. Sulla base di queste osservazioni, abbiamo usato la Multiplex Ligation-dependent Probe Amplification (MLPA) per r…
Modelling the Interaction between Air Pollutant Emissions and Their Key Sources in Poland
2021
The main purpose of this study is to investigate the relationships between key sources of air pollutant emissions (sources of energy production, factories which are particularly harmful to the environment, the fleets of cars, environmental protection expenditure) and the main environmental air pollution (SO2, NOx, CO and PM) in Poland. Models based on MLP neural networks were used as predictive models. Global sensitivity analysis was used to demonstrate the significant impact of individual network input variables on the output variable. To verify the effectiveness of the models created, the actual data were compared with the data obtained through modelling. Projected courses of changes in t…
Genetic profile and immunohistochemical study of clear cell renal carcinoma: Pathological-anatomical correlation and prognosis.
2021
Abstract Introduction Renal cell carcinoma (RCC) accounts for 2–3% of all tumors being the most frequent solid lesion in the kidney. Objective To determine what genetic alterations and immunohistochemical (IHC) of clear cell renal carcinoma (ccRCC) are associated with prognosis and tumor aggressiveness. Patients and Methods Experimental analytical study with 57 patients who underwent radical and partial nephrectomy between 2005 and 2011, all with diagnosis of ccRCC and minimum post-operative follow-up of 36 months. The pathological study included IHC determination of biomarkers associated (CAIX, CAM 5.2, CD10, c-erbB-2, EGFR, HIF-1a, Ki67, MDM2, PAX-2 y 8, p53, survivin and VEGFR 1 and 2). …