Search results for "Map"
showing 10 items of 3484 documents
Chromatin structure of transposon Tn903 cloned into a yeast plasmid
1989
Transposon Tn903 contains the APH gene for kanamycin resistance, which is active in yeast [A. Jiménez and J. Davies (1980) Nature (London) 287, 869-871] and is flanked by two inverted repeats (IR) 1057 bp long. When plasmid pAJ50, carrying Tn903 and the 2-microns circle origin of replication, is cloned into Saccharomyces cerevisiae, nucleosomes are assembled in vivo on the prokaryotic DNA of the transposon. Indirect end labeling revealed that three nucleosomes are preferentially positioned on symmetrical sequences from both IRs. DNase I digestion also confirmed that the chromatin structure is symmetrical in both IRs. This suggests that sequence determinants are decisive for chromatin struct…
The sex determining region of Chironomus thummi is associated with highly repetitive DNA and transposable elements.
1993
The dominant male sex determiner in chromosome III of the midge Chironomus thummi thummi is closely linked to a large cluster of tandem-repetitive DNA elements, the Cla elements, which are otherwise highly repetitive and distributed over more than 200 sites on all chromosomes. Chromosome III displays a hemizygous cluster of Cla elements in males but not in females. The chromosomal location of this hemizygous Cla element cluster is in the region of the male determiner M as localized by cytogenetic analysis. With Cla elements as hybridization probe, it was possible to clone a large part of the sex determining region. Molecular analysis of the DNA of males and females in this region displayed …
P sequences ofDrosophilla Subobscuralack exon 3 and may encode a 66 kd repressor-like protein
1991
Abstract Several P homologous sequences have been cloned and sequenced from Drosophila subobscura. These sequences are located at the 85DE region of the O chromosome and at least three of them are organized in tandem. We have identified four copies which exhibit strong similarity between them. All of the isolated elements are truncated at the 5' and 3' ends. They have lost the inverted terminal repeats and exon 3, but maintain exons 0, 1 and 2. They are transcribed producing a polyadenylated RNA. The structure of these transcripts suggests that they are able to encode a 66 kd repressor-like protein, but not a functional transposase. We ask about the biological role of a potential repressor …
The Mu1 transposable element of maize contains two promoter signals recognized by the Escherichia coli RNA polymerase.
1990
The galactokinase (GalK) expression plasmid vector system pKO-1 has been used to screen for promoter elements in the maize transposable element Mu1 that function in Escherichia coli. Two transcriptional start points, named S1 and S2, were identified, which are located in the two direct repeats of the transposable element. This paper demonstrates that sequence elements exist in a plant transposable element which function as prokaryotic promotors.
Acidic Environment Leads to ROS-Induced MAPK Signaling in Cancer Cells
2011
Tumor micromilieu often shows pronounced acidosis forcing cells to adapt their phenotype towards enhanced tumorigenesis induced by altered cellular signalling and transcriptional regulation. In the presents study mechanisms and potential consequences of the crosstalk between extra- and intracellular pH (pH(e), pH(i)) and mitogen-activated-protein-kinases (ERK1/2, p38) was analyzed. Data were obtained mainly in AT1 R-3327 prostate carcinoma cells, but the principle importance was confirmed in 5 other cell types. Extracellular acidosis leads to a rapid and sustained decrease of pH(i) in parallel to p38 phosphorylation in all cell types and to ERK1/2 phosphorylation in 3 of 6 cell types. Furth…
Genetics of maximal walking speed and skeletal muscle characteristics in older women.
2008
AbstractThe aim of this study was to examine whether maximal walking speed, maximal isometric muscle strength, leg extensor power and lower leg muscle cross-sectional area (CSA) shared a genetic effect in common. In addition, we wanted to identify the chromosomal areas linked to maximal walking speed and these muscle characteristics and also investigate whether maximal walking speed and these three skeletal muscle characteristics are regulated by the same chromosomal areas. We studied 217 monozygotic (MZ) and dizygotic (DZ) female twin pairs aged 66 to 75 years in the Finnish Twin Study on Aging study. The DZ pairs (94) were genotyped for 397 microsatellite markers in 22 autosomes and X-chr…
Spatial Representation of Plant Diversity at Geographical Scale: The Italian Experience
2021
The first attempts to draw up plant biodiversity maps in Italy are identified, some of which represent re-workings of data collected for chorological atlases of geographic areas of different extents. These were based particularly on the UTM grid, introduced and codified in Italy by Pignatti [6]. The limited examples made in Italy concern regional, provincial or district areas, some also in protected natural areas. As examples considered original, the maps developed at the Phytotaxonomy and Phytogeography Laboratory of the University of Palermo are presented. These involve the province of Palermo, the Madonie Natural Park in Palermo province, and the eastern Sicani mountains. The map of this…
Enhancing visual literacy through interpretation of photo-genres: toward a genre typology of journalistic photographs
2016
ABSTRACTAlthough genre is one of the most extensively employed concepts in various disciplines, only a few attempts have been made to classify images in the press, providing either a palette of thematic categories similar to those of photography competitions or theory-based classifications, which often ignore certain types of photographs. In addition, no studies have suggested the genre typology as a tool for the interpretation of journalistic photographs. Based on a literature review and visual material analysis from the Polish daily Gazeta Wyborcza, this study compiles a typology that sets out four photo-genres: news photography, reportage photography, portrait photography and illustrativ…
Opettajan työhyvinvoinnin muutos : opettajien kokemuksia väistöolosuhteiden työhyvinvoinnista
2017
TIIVISTELMÄ Ratia, Katja. 2017. Opettajan työhyvinvoinnin muutos: opettajien kokemuksia väistöolosuhteiden työhyvinvoinnista. Kasvatustieteen pro gradu -tutkielma. Jyväskylän yliopisto. Opettajankoulutuslaitos. 66 sivua. Tutkimuksen tarkoituksena oli selvittää opettajien työhyvinvoinnin muutoksia siirryttäessä työskentelemään väistötiloihin. Tavoitteena oli tuoda esille koke-muksia työympäristön muutoksesta ja kuvailla, millaista väistötilojen työhy-vinvointi on. Tutkimus toteutettiin laadullisena haastattelututkimuksena. Jou-lukuussa 2016 haastateltiin 3 opettajaa ja lisäksi kaksi opettajaa kirjoitti tiiviin kirjoitelman teemahaastattelurungon pohjalta. Aineisto kerättiin yhteensä 5 opetta…
A case study of a precision fertilizer application task generation for wheat based on classified hyperspectral data from UAV combined with farm histo…
2013
Different remote sensing methods for detecting variations in agricultural fields have been studied in last two decades. There are already existing systems for planning and applying e.g. nitrogen fertilizers to the cereal crop fields. However, there are disadvantages such as high costs, adaptability, reliability, resolution aspects and final products dissemination. With an unmanned aerial vehicle (UAV) based airborne methods, data collection can be performed cost-efficiently with desired spatial and temporal resolutions, below clouds and under diverse weather conditions. A new Fabry-Perot interferometer based hyperspectral imaging technology implemented in an UAV has been introduced. In this…