Search results for "Matrix"

A multi-methodology and sustainability-supporting framework for implementation and assessment of a holistic building renovation

La ristrutturazione degli edifici in futuro dovrà essere condotta secondo una prospettiva più olistica legata alla sostenibilità vista in una più ampia gamma di obiettivi/criteri e facilitata dagli scenari di ristrutturazione possibili. La ristrutturazione degli edifici dovrebbe servire a migliorarne le performance al fine di soddisfare le esigenze degli utenti, rendendo questi ultimi meno vulnerabili in relazione ai futuri costi energetici. Vi è un grande potenziale per ridurre il consumo di energia negli edifici esistenti. Tuttavia, ciò non deve comprometterè i valori architettonici e di qualità che rendono particolari. Pertanto, non possono essere semplicemente rinnovati, ma devono subir…

Solubilized liver extracellular matrix maintains primary rat hepatocyte phenotype in-vitro.

2015

Whole organ engineering and cell-based regenerative medicine approaches are being investigated as potential therapeutic options for end-stage liver failure. However, a major challenge of these strategies is the loss of hepatic specific function after hepatocytes are removed from their native microenvironment. The objective of the present study was to determine if solubilized liver extracellular matrix (ECM), when used as a media supplement, can better maintain hepatocyte phenotype compared to type I collagen alone or solubilized ECM harvested from a non-liver tissue source. Liver extracellular matrix (LECM) from four different species was isolated via liver tissue decellularization, solubil…

A symmetric Galerkin BEM for plate bending analysis

2009

Abstract The Symmetric Galerkin Boundary Element Method is employed in thin plate bending analysis in accordance with the Love–Kirchhoff kinematical assumption. The equations are obtained through the stationary conditions of the total potential energy, written for a plate whose boundary is discretized in boundary elements. Since the matrix coefficients are made up as double integrals with high order singularities, a strategy is shown to compute these coefficients in closed form. Furthermore, in order to model the kinematical discontinuities and to weight the mechanical quantities along the boundary elements, the Lagrangian quadratic shape functions, rather than C 1 type (spline, Hermitian),…

The generalized H-point standard-additions method to determine analytes present in two different chemical forms in unknown matrix samples. Part I. Ge…

2000

This paper shows how the generalized H-point standard-additions method (GHPSAM) can be used to obtain the total concentration or concentrations of different chemical forms of an analyte when the matrix of the sample is completely unknown. The spectral regions where the interferent behaviour can be considered as linear are found and the analyte concentration free from bias error is estimated. This paper includes the already published features of the GHPSAM and a new modification of this method which allows the simultaneous determination of two chemical forms of an analyte in a sample.

2018

We show that the measured intrinsic octupole moments of $^{220}\mathrm{Rn}$, $^{224}\mathrm{Ra}$, and $^{226}\mathrm{Ra}$ constrain the intrinsic Schiff moments of $^{225}\mathrm{Ra}$, $^{221}\mathrm{Rn}$, $^{223}\mathrm{Rn}$, $^{223}\mathrm{Fr}$, $^{225}\mathrm{Ra}$, and $^{229}\mathrm{Pa}$. The result is a dramatically reduced uncertainty in intrinsic Schiff moments. Direct measurements of octupole moments in odd nuclei will reduce the uncertainty even more. The only significant source of nuclear-physics error in the laboratory Schiff moments will then be the intrinsic matrix elements of the time-reversal noninvariant interaction produced by $CP$-violating fundamental physics. Those matri…

Quantitation of antigen-reactive T cells in peripheral blood by IFNgamma-ELISPOT assay and chromium-release assay: a four-centre comparative trial

2000

The ELISPOT assay is increasingly being used for the monitoring of the induction of antigen-reactive T cells in cancer vaccination trials. In order to evaluate the reliability of T cell frequency analysis with the ELISPOT assay, a comparative study was performed in four European laboratories. Six samples from healthy subjects were analyzed for the frequency of influenza-reactive CD8+ T cells in peripheral blood mononuclear cells (PBMC) by IFNgamma-ELISPOT assay. In addition, one laboratory determined cytotoxic T cell precursor (CTL) frequencies in these samples by limiting dilution chromium-release assay (LDA), and three laboratories performed a variant of the LDA, the multiple microculture…

Identification of T cell epitopes by the use of rapidly generated mRNA fragments.

2004

Abstract Although the number of defined T cell epitopes of clinically relevant antigens is constantly increasing, there is still an enormous need to identify further peptides, processed from new antigens or presented by rare HLA molecules, respectively. Here we introduce a novel two-step approach for the rapid identification of T cell epitopes. It was established in the CMV infection model. From the peripheral blood of healthy donors sharing HLA-A1 according to HLA serotyping we isolated CD8 + T lymphocytes and generated dendritic cells (DCs). DCs were electroporated with CMV pp65 mRNA and tested for recognition by autologous CD8 + T lymphocytes in IFN-γ ELISPOT assays. In all 10 CMV-seropo…

The use of clonal mRNA as an antigenic format for the detection of antigen-specific T lymphocytes in IFN-gamma ELISPOT assays.

2003

Abstract Most assay systems for the quantification of antigen-specific T-cell responses in infectious, malignant and autoimmune disease depend on the peptide antigen format and are therefore restricted to known epitopes and their presenting HLA molecules. Here we tested in ELISPOT assays the application of in vitro-transcribed clonal mRNA as an alternative antigen format covering all potential epitopes of a given antigen. As model antigens, we chose pp65 of human cytomegalovirus (HCMV) and human tyrosinase (hTyr). Antigen-presenting cells (APC) were K562 cells stably transfected with single HLA class I alleles and autologous dendritic cells (DC). As effectors, we applied in vitro-generated …

A New Approach to the Modeling of Anisotropic Media with the Transmission Line Matrix Method

2021

A reformulation of the Transmission Line Matrix (TLM) method is presented to model non-dispersive anisotropic media. Two TLM-based solutions to solve this problem can already be found in the literature, each one with an interesting feature. One can be considered a more conceptual approach, close to the TLM fundamentals, which identifies each TLM in Maxwell’s equations with a specific line. But this simplicity is achieved at the expense of an increase in the memory storage requirements of a general situation. The second existing solution is a more powerful and general formulation that avoids this increase in memory storage. However, it is based on signal processing techniques and considerabl…

A Transition Zone Complex Regulates Mammalian Ciliogenesis and Ciliary Membrane Composition

2011

Mutations in genes encoding ciliary components cause ciliopathies, but how many of these mutations disrupt ciliary function is unclear. We investigated Tectonic1 (Tctn1), a regulator of mouse Hedgehog signaling, and found that it is essential for ciliogenesis in some, but not all, tissues. Cell types that do not require Tctn1 for ciliogenesis require it to localize select membrane-associated proteins to the cilium, including Arl13b, AC3, Smoothened and Pkd2. Tctn1 forms a complex with multiple ciliopathy proteins associated with Meckel (MKS) and Joubert (JBTS) syndromes, including Mks1, Tmem216, Tmem67, Cep290, B9d1, Tctn2, and Cc2d2a. Components of the Tectonic ciliopathy complex colocaliz…