Search results for "Measles virus"
showing 6 items of 16 documents
Amino acids requirements of measles virus in HeLa cells.
1973
A low plating efficiency for measles virus was obtained when arginine, threonine and isoleucine were omitted from Eagle's basal medium and a stimulatory effect on the plaque production was produced by increasing the concentration up to 0.5 mm of arginine and glutamine. However, of all amino acids in Eagle's basal medium, only methionine was found to be essential for the synthesis of infectious progeny virus. Omission of any one of the other amino acids from the culture medium permitted limited virus production, but none whatever could be detected in the absence of mediummethionine. Moreover, when methionine was restored to infected cultures after 24 hours of deprivation, release of virus be…
Latent measles virus infection in Vero cells depending on a temperature-sensitive phenomenon.
1978
A latent infection by measles virus in a line of Vero cells could be maintained only at 37 degrees C. The conditions of temperature nonpermissiveness were associated with some block in virus production and/or release and with the establishment of an autointerference phenomenon. Reduction of the incubation temperature to 33.5 degrees C induced a rather rapid transition from the latent to a lytical infection with a recue of virus. The rescued virus exhibited a restricted capacity to grow at 37 degrees C.
Studies on a measles virus variant inducing persistent infections in cultured cells
1976
Attempts were made to characterized by a plaque assay two variants of the Edmonston strain of measles virus and to obtain plaque purified virus populations. The UP non-cytocidal variant, in all the examined cell systems, mainly produced small but also large plaques; the DP cytocidal variant always large plaques. Three clones, UP-SP4, UP-LP4 and DP-LP4, were derived by plaque purfication respectively of the UP small plaque, UP large plaque and DP large plaque forming particles. The virus populations of the clones could be distinguished by some other biological and physical characters: cytopathic effect in roller tube cultures, growth potential in HeLa cells, thermal stability at 45 degrees C…
Neurotropism in newborn hamsters of plaque purified measles virus clones
1976
Three plaque purified measles virus clones displayed a different neurotropism in newborn hamsters.
Inhibition of growth of measles virus by mycoplasma in cell-cultures and the restoring effect of arginine
1970
Presence of mycoplasma in MK cell cultures caused an inhibition of measles virus, as revealed by a lower plating efficiency. When arginine is supplemented to the cell-system, mycoplasma contaminated cells yield plaque titers as high as those of uncontaminated cells. The essential role of arginine for plaque formation of measles virus is suggested also by experiments in which arginine added to the agar overlay restored the plating efficiency of measles virus in cultures maintained in a minimal nutrient medium.
On the hemagglutinating and hemolytic activity of measles virus variants.
1967
The hemagglutinating (HA) and hemolytic (HL) activity of two measles virus variants, differing with regard to type of CPE and other characteristics, have been investigated.