Search results for "MerA"
showing 10 items of 3426 documents
Abundance of narG , nirS , nirK , and nosZ Genes of Denitrifying Bacteria during Primary Successions of a Glacier Foreland
2006
ABSTRACT Quantitative PCR of denitrification genes encoding the nitrate, nitrite, and nitrous oxide reductases was used to study denitrifiers across a glacier foreland. Environmental samples collected at different distances from a receding glacier contained amounts of 16S rRNA target molecules ranging from 4.9 × 10 5 to 8.9 × 10 5 copies per nanogram of DNA but smaller amounts of narG , nirK , and nosZ target molecules. Thus, numbers of narG , nirK , nirS , and nosZ copies per nanogram of DNA ranged from 2.1 × 10 3 to 2.6 × 10 4 , 7.4 × 10 2 to 1.4 × 10 3 , 2.5 × 10 2 to 6.4 × 10 3 , and 1.2 × 10 3 to 5.5 × 10 3 , respectively. The densities of 16S rRNA genes per gram of soil increased with…
Cannabinoid type 1 receptor modulates intestinal propulsion by an attenuation of intestinal motor responses within the myenteric part of the peristal…
2007
Cannabinoid-1 (CB1) receptor activation affects gastrointestinal propulsion in vivo. It was our aim to further characterize the involved myenteric mechanisms in vivo and in vitro. In CB1(-/-) mice and wild-type littermates we performed in vivo transit experiments by charcoal feeding and in vitro electrophysiological recordings in mouse small intestinal smooth muscle. Ascending neuronal contraction (ANC) following electrical field stimulation was studied in rat ileum in a partitioned organ bath separating the aboral stimulation site from the oral recording site. The knockout animals displayed an accelerated upper gastrointestinal transit compared to control animals. The CB1 receptor antagoni…
Inducible ASABF-Type Antimicrobial Peptide from the Sponge Suberites domuncula: Microbicidal and Hemolytic Activity in Vitro and Toxic Effect on Moll…
2011
Since sponges, as typical filter-feeders, are exposed to a high load of attacking prokaryotic and eukaryotic organisms, they are armed with a wide arsenal of antimicrobial/cytostatic low-molecular-weight, non-proteinaceous bioactive compounds. Here we present the first sponge agent belonging to the group of ASABF-type antimicrobial peptides. The ASABF gene was identified and cloned from the demospongeSuberites domuncula. The mature peptide, with a length of 64 aa residues has a predicted pI of 9.24, and comprises the characteristic CSαβ structural motif. Consequently, the S. domuncula ASABF shares high similarity with the nematode ASABFs ; it is distantly related to the defensins. The recom…
Selective downregulation of the MDR1 gene product in Caco-2 cells by stable transfection to prove its relevance in secretory drug transport.
2005
Considerable interest is focused on overcoming multidrug resistance (MDR) in cancer chemotherapy. The in vitro experiments to characterize P-glycoprotein's (P-gp) function and to decrease its effects have led to a variety of strategies such as addition of competitors or supplementation of the medium with oligonucleotides complementary to the 5'-end of the MDR1-mRNA. For the Caco-2 cell line, an in vitro model for absorption screening, expressing multiple transporters including P-gp, which pumps substances back into the apical solution, P-gp activity might mask other relevant transport proteins' activity. The objective of the present study was to construct a Caco-2 subline with reduced P-gp …
A melanocortin 1 receptor (MC1R) gene polymorphism is useful for authentication of Massese sheep dairy products
2011
Massese is an Italian sheep breed, with black or grey coat colour, mainly reared in the Tuscany and Emilia Romagna regions. Recently, the emerging interests in this breed have resulted in the production of Pecorino cheese obtained with only Massese milk. In order to be profitable, this marketing link between Massese breed and its products should be defended against fraudsters who could include milk of other sheep breeds or cow milk in Massese labelled productions. To identify the genetic factors affecting coat colour in sheep, we have recently analysed the melanocortin 1 receptor (MC1R) gene and identified several single nucleotide polymorphisms (SNPs). In this work, as a first step to set …
Educación compensatoria : aprendizaje temprano, éxito posterior : (II: el Carolina Abecedarian Project)
2014
Este es un estudio de revisión en el que se analizan las investigaciones que evalúan los efectos a lo largo del tiempo del programa de educacióntemprano Abecedarian Project (ABC). El ABC es un programa compensador preventivo de 0-5 años y entresacados de madres de alto riesgo. El grupo experimental (E) recibe tratamiento preescolar intensivo, no el control (C). Se prolongó escolarmente hasta los 8 años, subdividiéndose ambos grupos: Dos -EE y CE- recibieron tratamiento intensivo; otros (EC y CC) convencional. Fueron medidos hasta los 21. Resultados: Los jóvenes adultos, tratados preescolarmente, mejoraron en rendimiento mental, lector y matemático; alcanzaron mayores instrucciones y presenc…
DISTRIBUTION OF CARBON STARS IN THE GALAXY
2018
A search for new faint carbon (C) stars in the Polar region δ > 55o has been accomplished by obtaining objective prism spectra in the near infrared 550 -- 900 nm on images of CCD camera of Baldone Schmidt telescope of Astrophysical observatory. The positions of stars having color indices (J – K) > 1.3 mag in Two Micron All Sky Infrared Survey (2MASS) were selected to pick out suspicious carbon stars.Our survey is limited in brightness by J < 10 mag. 24 new carbon stars were found and their characteristics obtained. The correlative relation between Teff and spectral gradient [757 – 685] was confirmed. Such characteristics as Teff, distance from the Sun, absolute Mk were obtained…
The effects of water absorption and salt fog exposure on agglomerated cork compressive response
2022
The replacement of synthetic foams with agglomerated cork in sandwich composites can meet the increasing environmental concerns. Its peculiar morphology and chemical composition lead to outstanding dimensional recovery that endorsed a broad investigation of its compressive behavior. The knowledge of neat material response is fundamental to obtain a reliable design dataset, but it is necessary to consider all the environmental factors (water, moisture and sunlight) that significantly modify material mechanical properties. In view of this, the present work investigates the effect of distilled and seawater absorption and salt fog exposure on the compressive behavior of two agglomerated corks w…
Dual targeting of higher-order DNA structures by azacryptands induces DNA junction-mediated DNA damage in cancer cells
2021
Abstract DNA is intrinsically dynamic and folds transiently into alternative higher-order structures such as G-quadruplexes (G4s) and three-way DNA junctions (TWJs). G4s and TWJs can be stabilised by small molecules (ligands) that have high chemotherapeutic potential, either as standalone DNA damaging agents or combined in synthetic lethality strategies. While previous approaches have claimed to use ligands that specifically target either G4s or TWJs, we report here on a new approach in which ligands targeting both TWJs and G4s in vitro demonstrate cellular effects distinct from that of G4 ligands, and attributable to TWJ targeting. The DNA binding modes of these new, dual TWJ-/G4-ligands w…
Molecular epidemiology of Acinetobacter baumannii in Iran: endemic and epidemic spread of multiresistant isolates
2014
Objectives We examined the molecular epidemiology of Acinetobacter baumannii clinical isolates from two cities (Tehran and Tabriz) of Iran. Methods DiversiLab repetitive extragenic palindromic PCR (rep-PCR), multilocus sequence typing and sequence group multiplex PCR were performed. The presence of resistance mechanisms including metallo-β-lactamases, extended-spectrum β-lactamases, OXA carbapenemases, aminoglycoside-modifying enzymes and RNA methylases was also investigated. Results DiversiLab rep-PCR identified 11 clusters and 11 singleton isolates. Twelve sequence types (STs), including six novel types, were identified. Sequence groups (SGs) 1-3 as well as five additional banding pattern…