Search results for "Method"

showing 10 items of 13253 documents

Dysfunction of Oskyddad causes Harlequin-type ichthyosis-like defects in Drosophila melanogaster.

2020

Prevention of desiccation is a constant challenge for terrestrial organisms. Land insects have an extracellular coat, the cuticle, that plays a major role in protection against exaggerated water loss. Here, we report that the ABC transporter Oskyddad (Osy)—a human ABCA12 paralog—contributes to the waterproof barrier function of the cuticle in the fruit fly Drosophila melanogaster. We show that the reduction or elimination of Osy function provokes rapid desiccation. Osy is also involved in defining the inward barrier against xenobiotics penetration. Consistently, the amounts of cuticular hydrocarbons that are involved in cuticle impermeability decrease markedly when Osy activity is reduced. …

Cancer ResearchLife CyclesEmbryologyMutantCell MembranesATP-binding cassette transporterQH426-470Biochemistry0302 clinical medicineLarvaeAnimal WingsLoss of Function MutationMedicine and Health SciencesDrosophila ProteinsAnimal AnatomyGenetics (clinical)Barrier functionSkin0303 health sciencesbiologyDrosophila MelanogasterEukaryotaAnimal ModelsHarlequin IchthyosisLipidsCell biologyInsectsExperimental Organism SystemsEmbryology and OrganogenesisDrosophilaDrosophila melanogasterCellular Structures and OrganellesAnatomyIntegumentary SystemEmbryologie et organogenèseDrosophila ProteinAutre (Sciences du Vivant)Research Article[SDV.OT]Life Sciences [q-bio]/Other [q-bio.OT]ArthropodaResearch and Analysis Methods03 medical and health sciencesModel OrganismsExtracellularGeneticsAnimalsABCA12DesiccationMolecular BiologyEcology Evolution Behavior and Systematics030304 developmental biologyEmbryosfungiOrganismsBiology and Life SciencesCell Biologybiology.organism_classificationInvertebrates[SDV.BDD.EO]Life Sciences [q-bio]/Development Biology/Embryology and Organogenesisbiology.proteinAnimal StudiesATP-Binding Cassette TransportersEpidermisZoology030217 neurology & neurosurgeryIchthyosis LamellarDevelopmental BiologyPLoS Genetics
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Look for methods, not conclusions

2019

Qualunque sia l'esito effettivo di un'indagine su un caso di presunta manipolazione dei dati, ci sono alcuni principi fermi che sono sempre veri e alcuni errori che devono essere evitati.

Cancer ResearchMolecular biologyPeer ReviewScienceImmunologyScientific MisconductSettore AGR/13 - Chimica Agrariamethods; verification experimental datasetresearch integrityDiseasesmethodsCellular and Molecular NeuroscienceCorrespondenceScientific methodHumansexperimental datasetlcsh:QH573-671Scientific misconductPeer Review Researchlcsh:CytologyResearchCell BiologyEngineering ethicsverificationPsychologyHumans; Peer Review Research; Science; Scientific Misconduct
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Analyse économique des essais cliniques internationaux en cancérologie

2018

International audience; In oncology, as in other fields of medicine, international multicentre clinical trials came into being so as to include a sufficient number of subjects to investigate a clinical situation. The existence of tight budgetary constraints and the desire to make the best use of the resources available have resulted in the development of economic evaluations associated with these trials, which, thanks to their level of evidence and their size, provide particularly relevant material. Nonetheless, economic evaluations alongside international clinical trials raise specific questions of methodology with regard to both the design and the analysis of the results. Indeed, the cost…

Cancer ResearchMéthodologie[SDV.CAN]Life Sciences [q-bio]/Cancer[ SDV.CAN ] Life Sciences [q-bio]/Cancer03 medical and health sciencesEssai clinique international0302 clinical medicineGoods and services[SDV.CAN] Life Sciences [q-bio]/CancerMonetary valueInternational clinical trialMethodsRadiology Nuclear Medicine and imaging030212 general & internal medicineBudget constraintComputingMilieux_MISCELLANEOUSIndividual countryÉvaluation économique.Public economics030503 health policy & services[ SDV.SPEE ] Life Sciences [q-bio]/Santé publique et épidémiologieHematologyGeneral MedicineEvidence-based medicineEconomic evaluation3. Good healthClinical trialEconomic dataOncologyWork (electrical)[SDV.SPEE] Life Sciences [q-bio]/Santé publique et épidémiologie[SDV.SPEE]Life Sciences [q-bio]/Santé publique et épidémiologieBusiness0305 other medical science
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Claudin-18 splice variant 2 is a pan-cancer target suitable for therapeutic antibody development

2008

Abstract Purpose: Antibody-based cancer therapies have emerged as the most promising therapeutics in oncology. The purpose of this study was to discover novel targets for therapeutic antibodies in solid cancer. Experimental Design: We combined data mining and wet-bench experiments to identify strictly gastrocyte lineage–specific cell surface molecules and to validate them as therapeutic antibody targets. Results: We identified isoform 2 of the tight junction molecule claudin-18 (CLDN18.2) as a highly selective cell lineage marker. Its expression in normal tissues is strictly confined to differentiated epithelial cells of the gastric mucosa, but it is absent from the gastric stem cell zone. …

Cancer ResearchPathologymedicine.medical_specialtymicemedicine.drug_classMolecular Sequence DataGene ExpressionBiologyMonoclonal antibodyMalignant transformationAntigenmedicineProtein IsoformsAnimalsHumansNeoplasms Glandular and EpithelialMembrane Proteins/geneticsAntibodies Monoclonal/immunologyProtein Isoforms/immunologyBase SequenceReverse Transcriptase Polymerase Chain ReactionNeoplasms Glandular and Epithelial/drug therapyAntibodies MonoclonalImmunotherapy ActiveMembrane ProteinsCancermedicine.diseaseFlow CytometryImmunohistochemistryImmunotherapy Active/methodsOncologyCancer cellClaudinsbiology.proteinCancer researchImmunohistochemistryAntibodyStem cell
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Quality, comparability and methods of analysis of data on childhood cancer in Europe (1978-1997): report from the Automated Childhood Cancer Informat…

2006

International audience; In collaboration with 62 population-based cancer registries contributing to the Automated Childhood Cancer Information System (ACCIS), we built a database to study incidence and survival of children and adolescents with cancer in Europe. We describe the methods and evaluate the quality and internal comparability of the database, by geographical region, period of registration, type of registry and other characteristics. Data on 88,465 childhood and 15,369 adolescent tumours registered during 1978-1997 were available. Geographical differences in incidence are caused partly by differences in definition of eligible cases. The observed increase in incidence rates cannot b…

Cancer ResearchPediatricsDatabases FactualMESH: RegistriesMESH : Child Preschool[ SDV.CAN ] Life Sciences [q-bio]/Cancer0302 clinical medicineMESH : ChildNeoplasmsMESH: ChildEpidemiologyMedicineMESH: NeoplasmsRegistries030212 general & internal medicineMESH: IncidenceChildeducation.field_of_studyIncidenceIncidence (epidemiology)ComparabilityMESH: Infant NewbornQuality - methods - childhood cancer - EuropeMESH : InfantMESH : AdultMESH: InfantMESH : Incidence3. Good healthEuropeMESH: Reproducibility of ResultsOncologyChild Preschool030220 oncology & carcinogenesisMESH: Survival AnalysisAdultmedicine.medical_specialtyAdolescentPopulationMESH : EuropeMEDLINE[SDV.CAN]Life Sciences [q-bio]/CancerMESH : Databases FactualMESH : Infant Newborn03 medical and health sciencesEnvironmental healthMESH : AdolescentHumanseducationSurvival analysisMESH: AdolescentMESH: Humansbusiness.industryMESH : Reproducibility of ResultsMESH: Child PreschoolMESH : HumansInfant NewbornInfantReproducibility of ResultsCancerMESH: Adultmedicine.diseaseSurvival AnalysisMESH: Databases FactualMESH : NeoplasmsData qualityMESH: EuropeMESH : Survival AnalysisbusinessMESH : Registries
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Salso-bromo-iodine thermal water: a nonpharmacological alternative treatment for postnasal drip-related cough in children with upper respiratory trac…

2018

Postnasal drip (PND)-related cough is a very common symptom in patients with upper respiratory tract infections (URTis). At present, there is not a standard treatment for postnasal drip and postnasal drip-related cough. The aim of this pilot study was to evaluate the efficacy of a specific salso-bromoiodine thermal water containing hyaluronic acid and grapefruit seed extract (SBI-H-GSE) comparing it with a normal saline solution in children with URTis who refer PND-related symptoms. The study was randomized, single-blind, and controlled. Study group (75 children) was treated with SBI-HGSE and control group (65 children) was treated with a normal saline solution; both compounds were administ…

Cancer ResearchPhysiologyEndocrinology Diabetes and MetabolismImmunologyPilot ProjectsSalso-bromo-iodine thermal water isotonic saline postnasal dripEndocrinologyPhysiology (medical)Immunology and AllergyHumansSingle-Blind MethodHyaluronic AcidChildRespiratory Tract InfectionsPlant Extractspostnasal dripSalso-bromo-iodine thermal water isotonic saline postnasal drip; Endocrinology Diabetes and Metabolism; Immunology and Allergy; Physiology; Immunology; Oncology; Endocrinology; Physiology (medical); Cancer ResearchSalso-bromo-iodine thermal waterDiabetes and MetabolismNasal MucosaOncologyCoughSeedsQuality of Lifeisotonic salineSaline SolutionCitrus paradisiIodineJournal of biological regulators and homeostatic agents
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EURECCA colorectal: multidisciplinary management: European consensus conference colon & rectum.

2014

Contains fulltext : 137861.pdf (Publisher’s version ) (Closed access) BACKGROUND: Care for patients with colon and rectal cancer has improved in the last 20years; however considerable variation still exists in cancer management and outcome between European countries. Large variation is also apparent between national guidelines and patterns of cancer care in Europe. Therefore, EURECCA, which is the acronym of European Registration of Cancer Care, is aiming at defining core treatment strategies and developing a European audit structure in order to improve the quality of care for all patients with colon and rectal cancer. In December 2012, the first multidisciplinary consensus conference about…

Cancer ResearchQuality Assurance Health CareColorectal cancerCancer development and immune defence Radboud Institute for Molecular Life Sciences [Radboudumc 2]Delphi methodSurgical oncologyFAMILIAL ADENOMATOUS POLYPOSISTumours of the digestive tract Radboud Institute for Molecular Life Sciences [Radboudumc 14]MedicineSHORT-COURSE RADIOTHERAPYRectal cancerQuality assurance; Colon cancer; Rectal cancer; Multidisciplinary teams; Consensus; Delphi method; Audit; Neoadjuvant treatment; Adjuvant treatment; SurgerySettore MED/36 - DIAGNOSTICA PER IMMAGINI E RADIOTERAPIADisease ManagementPHASE-III TRIALRANDOMIZED CONTROLLED-TRIALNeoadjuvant TherapyQuality assuranceColon cancerEuropemedicine.anatomical_structureTreatment OutcomeOncologyTRANSANAL ENDOSCOPIC MICROSURGERYColonic NeoplasmsPractice Guidelines as TopicHYPERTHERMIC INTRAPERITONEAL CHEMOTHERAPYmedicine.medical_specialtyNeoadjuvant treatmentEvidence-based practiceConsensusLYMPH-NODE EVALUATIONDelphi methodRectumAuditSDG 3 - Good Health and Well-beingHumansCIRCUMFERENTIAL RESECTION MARGINddc:610business.industryRectal NeoplasmsTOTAL MESORECTAL EXCISIONCancerLONG-TERM SURVIVALAuditAdjuvant treatmentmedicine.diseaseSurgeryOncology nursingFamily medicineSurgeryMultidisciplinary teamsbusiness
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A dosimetric study of Leipzig applicators.

2005

Purpose: To obtain the absolute dose-rate distribution in liquid water for all six cup-shaped Leipzig applicators by means of an experimentally validated Monte Carlo (MC) code. These six applicators were used in high-dose-rate (HDR) afterloaders with the “classic” and v2 192Ir sources. The applicators have an inner diameter of 1, 2, and 3 cm, with the source traveling parallel or perpendicular to the contact surface. Methods and materials: The MC GEANT4 code was used to obtain the dose-rate distribution in liquid water for the six applicators and the two HDR source models. To normalize the applicator output factors, a MC simulation for the “classic” and v2 sources in air was performed to es…

Cancer ResearchRadiationDosimeterbusiness.industryPhantoms Imagingmedicine.medical_treatmentBrachytherapyMonte Carlo methodBrachytherapyWaterRadiotherapy DosageIridium RadioisotopesImaging phantomKermaOpticsOncologyIonization chamberPerpendicularmedicineDosimetryRadiology Nuclear Medicine and imagingThermoluminescent DosimetrybusinessNuclear medicineMonte Carlo MethodInternational journal of radiation oncology, biology, physics
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Cell volume homeostatically controls the rDNA repeat copy number and rRNA synthesis rate in yeast

2021

[Abstract] The adjustment of transcription and translation rates to the changing needs of cells is of utmost importance for their fitness and survival. We have previously shown that the global transcription rate for RNA polymerase II in budding yeast Saccharomyces cerevisiae is regulated in relation to cell volume. Total mRNA concentration is constant with cell volume since global RNApol II-dependent nascent transcription rate (nTR) also keeps constant but mRNA stability increases with cell size. In this paper, we focus on the case of rRNA and RNA polymerase I. Contrarily to that found for RNA pol II, we detected that RNA polymerase I nTR increases proportionally to genome copies and cell s…

Cancer ResearchTranscription GeneticCellGene ExpressionRNA polymerase IIYeast and Fungal ModelsProtein SynthesisQH426-470HaploidyBiochemistryPolymerasesSirtuin 2Transcription (biology)RNA Polymerase IHomeostasisCell Cycle and Cell DivisionGenetics (clinical)Silent Information Regulator Proteins Saccharomyces cerevisiaebiologyTranscriptional ControlEukaryotaChemical SynthesisGenomicsCell biologyNucleic acidsmedicine.anatomical_structureExperimental Organism SystemsRibosomal RNARNA polymeraseCell ProcessesRNA Polymerase IIResearch ArticleCell biologyCellular structures and organellesSaccharomyces cerevisiae ProteinsBiosynthetic TechniquesSaccharomyces cerevisiaeSaccharomyces cerevisiaeResearch and Analysis MethodsDNA RibosomalSaccharomycesModel OrganismsCyclinsDNA-binding proteinsmedicineRNA polymerase IGeneticsGene RegulationNon-coding RNAMolecular BiologyEcology Evolution Behavior and SystematicsCell SizeMessenger RNACèl·lules eucariotesOrganismsFungiRNABiology and Life SciencesProteinsGenes rRNARibosomal RNAModels Theoreticalbiology.organism_classificationYeastGenòmicabiology.proteinAnimal StudiesRNARibosomes
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Assembling a consensus on actinic cheilitis: A Delphi study.

2021

Aims: To discuss the terminology to define and classify actinic cheilitis (AC) and to build a consensus on the diagnostic and therapeutic approaches to AC. Methods: Two-round Delphi study using a questionnaire including 34 closed sentences (9 on terminology and taxonomy, 5 on potential for malignant transformation, 12 on diagnostic aspects, 8 on treatment) and 8 open questions. Experts’ agreement was rated using a Likert scale (1–7). Results: A consensus was reached on 24 out 34 statements (73.5%) and on 5 out of 8 (62.5%) close-ended questions. The response rate was identical in both rounds (attrition of 0%). AC is the term with the highest agreement (median of 7 (strongly agree; IQR: 6–7)…

Cancer Researchmedicine.medical_specialtyConsensusDelphi TechniqueetiologyefficacyDelphi methodPalpationPathology and Forensic Medicinelip03 medical and health sciences0302 clinical medicinepotentially malignant disordersInternal medicineSurveys and QuestionnairesBiopsymedicineactinic cheilitisHumansResponse rate (survey)CO2-lasermedicine.diagnostic_testbusiness.industryActinic cheilitis030206 dentistrymedicine.diseasepotentially malignant oral disorderDelphi studyOtorhinolaryngologyphotodynamic therapyCheilitisHomogeneousconsensus030220 oncology & carcinogenesiskeratosisPeriodonticsOral SurgerybusinessJournal of oral pathologymedicine : official publication of the International Association of Oral Pathologists and the American Academy of Oral PathologyREFERENCES
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