Search results for "Microbio"
showing 10 items of 8741 documents
Crystallization and preliminary crystallographic analysis of the major capsid proteins VP16 and VP17 of bacteriophage P23-77.
2012
The major capsid proteins VP16 and VP17 of bacteriophage P23-77 have been crystallized using both recombinant and purified virus and preliminary diffraction analyses have been performed.
Preliminary study on the unusual properties in the habitat of Ranunculetum baudotii in Central Europe
2011
Abstract The study examines the patches of the saltwater association Ranunculetum baudotii in a new, atypical site; i.e., located more than 400 km away from the Baltic coastal line. They were found in a karst spring, and were the first locality of the community identified in Poland. The water and sediments within the patches were analyzed chemically in the laboratory, but, conductivity, water temperature and O2 content were measured in the field. The spring was determined to be a relatively stable aquatic habitat, with a typical chemical composition of subterranean water (a prevalence of hydro-carbonate, calcium and magnesium ions), and rich, e.g., in nitrogen and phosphorus, and disturbed …
Cities and their effects on free-living and host-associated microbes
2023
Mikrobit ovat oleellisen tärkeitä elämälle maapallolla, ja niitä esiintyy vapaina ympäristössä tai isäntäorganismeissa tukien ekosysteemien ja/tai isäntien normaalia toimintaa. Ihminen vaikuttaa lähes kaikkiin planeettamme elinympäristöihin aiheuttaen niiden häviämistä ja huonontumista (mukaan lukien kaupunkien rakentaminen) sekä ympäristöjen saastumista ja ilmastonmuutosta. Mikrobiyhteisöjen (kutsutaan myös mikrobiotaksi, mukaan lukien bakteerit ja sienet) odotetaan reagoivan näihin ihmisen aiheuttamiin valintapaineisiin sopeutumalla muuttuvaan ympäristöön. Siten ihmisen aiheuttamat muutokset ympäristössä voivat muovata mikrobiyhteisöjä (sekä vapaasti eläviä että isäntiin liittyviä) muutta…
Cardiorenal Syndrome: New Pathways and Novel Biomarkers
2021
Cardiorenal syndrome (CRS) is a multi-organ disease characterized by the complex interaction between heart and kidney during acute or chronic injury. The pathogenesis of CRS involves metabolic, hemodynamic, neurohormonal, and inflammatory mechanisms, and atherosclerotic degeneration. In the process of better understanding the bi-directional pathophysiological aspects of CRS, the need to find precise and easy-to-use markers has also evolved. Based on the new pathophysiological standpoints and an overall vision of the CRS, the literature on renal, cardiac, metabolic, oxidative, and vascular circulating biomarkers was evaluated. Though the effectiveness of different extensively applied biomark…
APPLICATION OF SELECTED LACTIC ACID BACTERIA FOR THE YEAR-ROUND PRODUCTION OF VASTEDDALIKE CHEESE
2014
I batteri lattici delle cariossidi del grano: influenza geografica, varietale e del periodo di invecchiamento
2021
Il presente articolo riporta i dati di alcune ricerche volte a risalire all’origine dei batteri lattici degli impasti acidi. A tal proposito, i batteri lattici del frumento di quattro varietà moderne sono stati monitorati a partire dalle spighe. Le cariossidi ospitavano livelli di microrganismi inferiori rispetto a quelli rilevati sulle spighe e queste ultime livelli inferiori rispetto alle semole. La minore biodiversità lattica è stata riscontrata sulle cariossidi, mentre le semole hanno mostrato la maggiore ricchezza di specie e ceppi. Al fine di valutare la resistenza dei batteri lattici del grano durante l’invecchiamento, le cariossidi di due varietà antiche e due moderne di grani duri …
Different Modes of Regulation of the Expression of Dextransucrase in
2019
Leuconostoc lactis AV1 strain isolated from a Tunisian avocado was characterized as a dextran producer. The promoter PdsrLL and the dsrLL gene encoding the DsrLL dextransucrase responsible for the dextran synthesis were transcriptionally fused to the mCherry coding gene generating the pRCR20 plasmid. Upon plasmid transfer, both AV1n and the dextran non-producing Leuconostoc mesenteroides CM70 became red due to expression of the mCherry from the PdsrLL-dsr-mrfp transcriptional fusion. Characterization of the polymers present in cultures supernatants revealed that the DsrLL encoded from pRCR20 in the recombinant bacteria was able to synthesize dextran. The production of dextran by the DsrLL i…
Dextransucrase Expression Is Concomitant with that of Replication and Maintenance Functions of the pMN1 Plasmid in
2017
The exopolysaccharide synthesized by Lactobacillus sakei MN1 is a dextran with antiviral and immunomodulatory properties of potential utility in aquaculture. In this work we have investigated the genetic basis of dextran production by this bacterium. Southern blot hybridization experiments demonstrated the plasmidic location of the dsrLS gene, which encodes the dextransucrase involved in dextran synthesis. DNA sequencing of the 11,126 kbp plasmid (pMN1) revealed that it belongs to a family which replicates by the theta mechanism, whose prototype is pUCL287. The plasmid comprises the origin of replication, repA, repB, and dsrLS genes, as well as seven open reading frames of uncharacterized f…
Sourdough and cereal-based foods: traditional and innovative products
2017
Cereals represent the main crop of the five continents and contribute greatly to the diet of several populations. Cereals often undergo a fermentation before eating. This process is fundamental to confer high sensory quality, as well as to preserve and enhance the nutritional and safety aspects of the resulting products. Fermented cereal based foods are complex microbial ecosystems, mainly represented by lactic acid bacteria and yeasts. Among these products, sourdough is worldwide applied to produce breads. Sourdough technology is also used to improve the final characteristics of fortified and gluten–free breads. This chapter focuses on the use of cereals to produce the most relevant fermen…
Analysis of the endometrial microbiome and its impact on human reproduction
2021
La implantación es un proceso complejo que requiere la sincronización entre un endometrio receptivo y un blastocisto en desarrollo. En los últimos años, gracias a los avances en las técnicas de secuenciación, se han identificado microorganismos en el útero y se ha visto que pueden tener un efecto sobre los resultados reproductivos. Por ello, el estudio del microbioma endometrial está ganando cada vez más interés en las clínicas de reproducción asistida. En este estudio, planteamos la hipótesis de que la presencia de patógenos bacterianos en el útero tiene consecuencias negativas en la salud reproductiva. El objetivo principal fue caracterizar en profundidad el microbioma endometrial y su im…