Search results for "Microscope"
showing 10 items of 1412 documents
Relationship between scanning near-field optical images and local density of photonic states
2001
From numerical calculations based on Green's dyadic formalism, we show that a scanning near-field optical microscope (SNOM) working with a point-like illuminating probe delivers images that contain features directly related to the local density of photonic states (LDOS). More precisely, an unambiguous identification of the partial LDOSs (x, y or z polarized) can be made in the SNOM images when the solid angle of detection reaches 2π sr.
Electron Microscopic Studies of Spruce Needles in Connection with the Occurrence of Novel Forest Decline.
1988
Needles of four spruce trees showing different degrees of novel kinds of forest decline were investigated by electron microscopy. Green needles appearing at least superficially still intact were selected for the present investigation. Most of the mesophyll appeared to be undamaged. However, groups of atypical mesophyll cells were found close to the endodermis or the hypodermis. The chloroplasts of the apparently damaged cells were particularly affected. Changes in the matrix of the chloroplasts, i.e,. increased affinity to osmium, occurrence of extensive nests of plastoglobuli, as well as damage to the membranes, i.e. lesions in the envelope and abnormal thylakoid membranes, were observed. …
Objective improvement of the visual quality of ion microscope images
2013
The need to operate with low ion beam fluences implies the images obtained using ion microscope (IM) are often grainy and have poor visual quality compared to what can be obtained using e.g. confocal microscopy. This results from the Poissonian distribution of counts in pixels. Here we report work on some different approaches for objectively improving the visual quality of IM images. In this work we present (i) dramatic improvement in the visual image quality of off-axis and direct-scanning transmission ion microscopy (STIM) images by suppression of zero-pixels; (ii) denoising of PIXE images using wavelet filtering and (iii) use of the feature preserving characteristics of wavelet filtering…
Surface structure determination by SEM image processing and electrochemical impedance of graphite+polyethylene composite electrodes
2004
Abstract The electrochemical impedance behaviour of heterogeneous or/and rough electrodes has usually been associated with some geometrical parameters of the surface structure or to a statistical description of the surface properties. An experimental method for sample preparation of graphite + polyethylene composite electrodes based on plasma etching is proposed. After plasma etching, the statistical properties of the electrode, such as the graphite particle size distribution and fractional coverage are measured from scanning electron microscopy pictures using image processing software. The statistical information obtained for the surface is combined with impedance equations of independent …
Localization of antigens PwA33 and La on lampbrush chromosomes and on nucleoplasmic structures in the oocyte of the urodele Pleurodeles waltl: Light …
1994
Monoclonal antibodies A33/22 and La11G7 have been used to study the distribution of the corre-sponding antigens, PwA33 and La, on the lampbrush chromosome loops and nucleoplasmic structures of P. waltl oocytes, using immunofluorescence, confocal laser scanning microscopy and immunogold labeling. The results obtained with these antibodies have been compared with those obtained with the Sm-antigen-specific monoclonal antibody Y12. All these monoclonal antibodies (mAbs) labeled the matrices of the majority of normal loops along their whole length. Nucleoplasmic RNP granules showed a strong staining with the mAbs La11G7 and Y12 throughout their mass, but with the mAb A33/22, they showed only a …
Quasi-spherical focal spot in two-photon scanning microscopy by three-ring apodization
2005
International audience; We present a beam-shaping technique for two-photon excitation (TPE) fluorescence microscopy. We show that by inserting a properly designed three-ring pupil filter in the illumination beam of the microscope, the effective optical sectioning capacity of such a system improves so that the point spread function gets a quasi-spherical shape. Such an improvement, which allows the acquisition of 3D images with isotropic quality, is obtained at the expense of only a small increase of the overall energy in the axial sidelobes. The performance of this technique is illustrated with a scanning TPE microscopy experiment in which the image of small beads is obtained. We demonstrat…
Fast multi-directional DSLM for confocal detection without striping artifacts
2020
In recent years light-sheet fluorescence microscopy (LSFM) has become a cornerstone technology for neuroscience, improving the quality and capabilities of 3D imaging. By selectively illuminating a single plane, it provides intrinsic optical sectioning and fast image recording, while minimizing out of focus fluorescence background, sample photo-damage and photo-bleaching. However, images acquired with LSFM are often affected by light absorption or scattering effects, leading to un-even illumination and striping artifacts. In this work we present an optical solution to this problem, via fast multi-directional illumination of the sample, based on an acousto-optical deflector (AOD). We demonstr…
Axial resolution in two-color excitation fluorescence microscopy by phase-only binary apodization
2005
We study the effect of a kind of binary phase-only filters, the Toraldo filters, in two-color excitation fluorescence microscopy. We show that by simple insertion of a properly designed Toraldo filter in one of the illumination arms the axial resolution of the system is significantly improved. Specifically, the main peak of the point spread function is narrowed by 22% along the axial direction.
Long working range light field microscope with fast scanning multifocal liquid crystal microlens array
2018
The light field microscope has the potential of recording the 3D information of biological specimens in real time with a conventional light source. To further extend the depth of field to broaden its applications, in this paper, we proposed a multifocal high-resistance liquid crystal microlens array instead of the fixed microlens array. The developed multifocal liquid crystal microlens array can provide high quality point spread function in multiple focal lengths. By adjusting the focal length of the liquid crystal microlens array sequentially, the total working range of the light field microscope can be much extended. Furthermore, in our proposed system, the intermediate image was placed i…
Sidelobe decline in single-photon 4Pi microscopy by Toraldo rings.
2003
We demonstrate theoretically the feasibility of single-photon 4Pi-confocal microscopy. By inserting a pair of properly designed multi-ring phase-only pupil filters in the illumination path of a 4Pi microscope the height of the sidelobes of the point spread function substantially reduced, so that there is no ambiguity in the 3D image. Then, an axial resolution up to four times higher than that of single-photon confocal microscope can be effectively achieved.