Search results for "Microscopy"
showing 10 items of 3390 documents
Hilbert-Huang single-shot spatially multiplexed interferometric microscopy.
2018
Hilbert-Huang single-shot spatially multiplexed interferometric microscopy (H2S2MIM) is presented as the implementation of a robust, fast, and accurate single-shot phase estimation algorithm with an extremely simple, low-cost, and highly stable way to convert a bright field microscope into a holographic one using partially coherent illumination. Altogether, H2S2MIM adds high-speed (video frame rate) quantitative phase imaging capability to a commercially available nonholographic microscope with improved phase reconstruction (coherence noise reduction). The technique has been validated using a 20×/0.46 NA objective in a regular Olympus BX-60 upright microscope for static, as well as dynamic…
Aberration compensation for objective phase curvature in phase holographic microscopy: comment
2014
In a recent Letter by Seo et al. [Opt. Lett. 37, 4976 (2012)], the numerical correction of the quadratic phase distortion introduced by the microscope objective in digital holographic microscopy (DHM) has been presented. In this comment, we would like to draw to the attention of the authors and the readers in general that this approach could not be the optimal solution for maintaining the accuracy of the quantitative phase via DHM. We recall that the use of telecentric imaging systems in DHM simplifies the numerical processing of the phase images and produces more accurate measurements.
Resolution improvements in integral microscopy with Fourier plane recording
2016
Abstract: Integral microscopes (IMic) have been recently developed in order to capture the spatial and the angular information of 3D microscopic samples with a single exposure. Computational post-processing of this information permits to carry out a 3D reconstruction of the sample. By applying conventional algorithms, both depth and also view reconstructions are possible. However, the main drawback of IMic is that the resolution of the reconstructed images is low and axially heterogeneous. In this paper, we propose a new configuration of the IMic by placing the lens array not at the image plane, but at the pupil (or Fourier) plane of the microscope objective. With this novel system, the spa…
Generalized bloch equations for optical interactions in confined geometries
2005
By combining the field-susceptibility technique with the optical Bloch equations, a general formalism is developed for the investigation of molecular photophysical phenomena triggered by nanometer scale optical fields in the presence of complex environments. This formalism illustrate the influence of the illumination regime on the fluorescence signal emitted by a single molecule in a complex environment. In the saturated case, this signal is proportional to the optical local density of states, while it is proportional to the near-field intensity in the non-saturated case. (C) 2005 Elsevier B.V. All rights reserved.
Shift-variant digital holographic microscopy: inaccuracies in quantitative phase imaging
2013
Inaccuracies introduced in quantitative phase digital holographic microscopy by the use of nontelecentric imaging systems are analyzed. Computer modeling of the experimental result shows that even negligible errors in the radius and center of curvature of the numerical compensation needed to get rid of the remaining quadratic phase factor introduce errors in the phase measurements; these errors depend on the position of the object in the field-of-view. However, when a telecentric imaging system is utilized for the recording of the holograms, the numerical modeling and experimental results show the shift-invariant behavior of the quantitative-phase digital holographic microscope.
Superresolution digital holographic microscopy for three-dimensional samples.
2009
An approach that allows superresolution imaging of three-dimensional (3-D) samples by numerical refocusing is presented in the field of digital holographic microscopy. Based on the object's spectrum shift produced by tilted illumination, we present a time multiplexing superresolved approach to overcome the Abbe's diffraction limit. The proposed approach uses a microscope in a Mach-Zehnder interferometric architecture with the particularity that the output plane does not coincide with the image plane. Thus, a set of off-axis non-image plane holograms are sequentially recorded for every tilted beam used in the illumination stage. After that and by using simple digital post-processing and nume…
Filter performance parameters for vectorial high-aperture wave fields.
2008
Performance parameters have been presented that can be used to compare the focusing performance of different optical systems, including the effect of pupil filters. These were originally given for the paraxial case and recently extended to the high-aperture scalar regime. We generalize these parameters to the full vectorial case for an aplanatic optical system illuminated by a plane-polarized wave. The behavior of different optical systems is compared.
Transmission of ultra-cold neutrons through guides coated with materials of high optical potential
2014
Abstract We have measured the transmission of ultra-cold neutrons (UCN) through tubes coated on the inside with materials of high optical potentials. The best transmission value, T, normalised to 1 m long UCN guides was obtained with a beryllium coated quartz guide, T=(99.0±1.0)%. Furthermore, excellent transmission coefficients were found for (i) a glass tube coated with NiMo(88/12), T=(97.3 ±0.6)%, (ii) a NiMo(85/15) guide made by the replication technique, T=(97.1 ±0.8)% and (iii) a glass guide coated with NiMo(82/18), T=(96.3 ±2.1)%. The results demonstrate that UCN guides made from coated glass tubes have the same excellent transmission properties as those produced with the replication…
Einzelnachweis von Elektronen (15 bis 50 keV) in Kernphotoplatten
1960
Nuclear track emulsions (Ilford G 5) were used for counting of single slow electrons. They had to be accelerated by high voltage of about 30 kV and were incident vertically. The pulsed electron-beam was measured in a cage, single pulses were shot on the emulsion. The developed plates were photographed under the microscope and the tracks counted on the enlarged copies. The results are: about 40% of incoming electrons can be found as tracks in the emulsions. This number varies between different emulsions (of same type G 5), within one plate the number of tracks is better proportional to the number of electrons than between different plates. With a counting resultx one may expect the true resu…
Nuclear magnetic resonance at millitesla fields using a zero-field spectrometer
2016
We describe new analytical capabilities for nuclear magnetic resonance (NMR) experiments in which signal detection is performed with chemical resolution (via spin-spin J couplings) in the zero to ultra-low magnetic field region, below 1μT. Using magnetic fields in the 100μT to 1mT range, we demonstrate the implementation of conventional NMR pulse sequences with spin-species selectivity.