Search results for "Myc"
showing 10 items of 3773 documents
Estudio de la reducción química y biológica de hongos y micotoxinas en pan
2017
El deterioro de los alimentos causado por los hongos que producen micotoxinas representa un problema importante en seguridad alimentaria. Los cereales en grano y sus productos derivados, como el pan, frecuentemente están contaminados con hongos micotoxigénicos. Es por ello que en la presente Tesis Doctoral se han estudiado la presencia de 17 micotoxinas en 80 muestras de pan de molde y el riesgo de exposición de la población a estos compuestos. Aflatoxinas (AFs), zearalenona (ZEA) y eniatinas (ENs) han sido detectadas respectivamente en el 20%, 65% y 96% de las muestras de pan analizadas. La presencia de dichas micotoxinas y, sobre todo, de las muestras en las que las AFs y la ZEA superan e…
2-feniletanola biosintēze ar raugu Kluyveromyces lactis laktozi saturošos substrātos
2018
Fermentācijas procesā raugi spēj sintezēt aromātiskus savienojumus, tāpēc fermentācija ar raugiem ir alternatīva metode dabīgā 2-feniletanola iegūšanai. Darba mērķis bija izpētīt rauga Kluyveromyces lactis izmantošanas iespējas 2-feniletanola biosintēzē laktozi saturošos substrātos. Darbā tika noteikts piemērotākais K. lactis celms 2-feniletanola biosintēzei, tika izpētīta barotnes sastāva ietekme uz 2-PE producēšanu, 2-PE sintēzes dinamika fermentācijas laikā, K. lactis DSM 70799 2-feniletanola tolerance, kā arī tika veikta divfāžu ekstraktīvā fermentācija ar rapšu eļļu. Darbā tika aplūkoti trīs rūpniecībā izmantojami K. lactis celmi, no kuriem K. lactis DSM 70799 uzrādīja augstāko 2-fenil…
Rauga Kluyveromyces marxianus biomasas producēšana laktozi saturošos substrātos
2015
Bakalaura darba mērķis bija izvērtēt aerācijas, temperatūras, rauga ekstrakta, melases, amonija sulfāta un urīnvielas ietekmi uz biomasas producēšanu un tās sastāvu laktozes saturošās barotnēs. Fermentācijām tika izvēlēts rauga Kluyveromyces marxianus DSM 5422 celms, kas uzrādīja labāku biomasas iznākumu, salīdzinot ar rauga Kluyveromyces lactis DSM 4394, DSM 70800 un DSM 70799 celmiem. Aerācijas diapazonā 0,2 – 2,0 L·L-1·min-1 optimāls biomasas iznākums tika novērots 1,0 L·L-1·min-1 aerācijas režīmā 35oC temperatūrā. Tika pētīta dažādu piedevu (rauga ekstrakta, melases, amonija sulfāta, urīnvielas) ietekme uz biomasas veidošanos. Rauga ekstrakta un melases pievienošana fermentācijas barotn…
Piena permeāta izmantošanas iespējas 2-feniletanola biosintēzei ar raugu Kluyveromyces marxianus
2017
Bakalaura darba mērķis bija izvērtēt industriāla substrāta – laktozi saturoša piena permeāta- izmantošanas iespējas 2-feniletanola biosintēzei ar rauga Kluyveromyces marxianus diviem celmiem- DSM 5422 un DSM 5418- un noskaidrot barotnes sastāva (pussintētiskā laktozes barotne, piena permeāta laktozes barotne, slāpekļa avota (amonija sulfāta), L-fenilalanīna koncentrācija) ietekmi uz 2-feniletanola producēšanu. Tika pierādīta piena permeāta kā oglekļa avota izmantošanas iespēja 2-feniletanola biosintēzei. Pētījumu gaitā tika noskaidrots, ka labākus 2-feniletanola biosintēzes rādītājus uzrādīja K.marxianus DSM 5418 celms, kurš tika audzēts piena permeāta barotnē bez papildus pievienotā amonij…
Sulfur transfer and activation by ubiquitin-like modifier system Uba4•Urm1 link protein urmylation and tRNA thiolation in yeast.
2017
Urm1 is a unique dual-function member of the ubiquitin protein family and conserved from yeast to man. It acts both as a protein modifier in ubiquitin-like urmylation and as a sulfur donor for tRNA thiolation, which in concert with the Elongator pathway forms 5-methoxy-carbonyl-methyl-2-thio (mcm5s2) modified wobble uridines (U34) in anticodons. Using Saccharomyces cerevisiae as a model to study a relationship between these two functions, we examined whether cultivation temperature and sulfur supply previously implicated in the tRNA thiolation branch of the URM1 pathway also contribute to proper urmylation. Monitoring Urm1 conjugation, we found urmylation of the peroxiredoxin Ahp1 is suppre…
Antagonism of Bacillus subtilis strain AG1 against vine wood fungal pathogens
2009
Summary. Antagonistic substances produced by a Bacillus subtilis strain (AG1), which were previously found to slow down the growth of esca fungi in vitro, were produced in an artifi cial medium, isolated from the cell-free medium by precipitation and acidifi cation (to less than pH 2.5) and extracted from the precipitate with 96% ethanol. The crude extract employed in antibiotic assays confi rmed, in vitro, the antagonism of B. subtilis against Phaeoacremonium aleophilum and Phaeomoniella chlamydospora, and also showed antifungal activity toward Verticillium dahliae and Botryosphaeria rhodina.
From microbial proteomics to synthetic biology: Amycolatopsis balhimycina case
2012
Actinomycetes, filamentous Gram-positive bacteria, are usually exploited as bio-farms naturally producing a wide range of small biologically active metabolites, such as antibiotics, extensively used in medicine, food-industry, chemistry and bio-remediation strategies. The development of high throughput technologies, like proteomics, allows functional genomic studies aimed at shedding light on molecular mechanisms controlling the production of useful compounds and macromolecules. Differential proteomic analyses, performed by using Two Dimensional PolyAcrylamide Gel Electrophoresis (2D-PAGE) coupled to mass spectrometry (MS) procedures, revealed novel links between balhimycin production (a va…
Azithromycin Differentially Alters TCR-Activated Helper T Cell Subset Phenotype and Effector Function
2020
In addition to their antibiotic activities, azithromycin (AZM) exhibits anti-inflammatory effects in various respiratory diseases. One of the potent anti-inflammatory mechanisms is through inhibition of CD4+ helper T (Th) cell effector function. However, their impact on specific Th subset is obscure. Herein, we demonstrate the cellular basis of phenotypic and functional alterations associated with Th subsets following AZM treatment in vitro. Using well-characterized Th subset specific chemokine receptors, we report significant suppression of T cell receptor (TCR)-stimulated hyperactivated CCR4+CXCR3+ (Th0) expansion compared to CCR4-CXCR3+ (Th1-like) and CCR4+CXCR3- (Th2-like) cells. Intere…
Mycobacterium tuberculosis Drives Expansion of Low-Density Neutrophils Equipped With Regulatory Activities
2019
In human tuberculosis (TB) neutrophils represent the most commonly infected phagocyte but their role in protection and pathology is highly contradictory. Moreover, a subset of low-density neutrophils (LDNs) has been identified in TB, but their functions remain unclear. Here, we have analyzed total neutrophils and their low-density and normal-density (NDNs) subsets in patients with active TB disease, in terms of frequency, phenotype, functional features, and gene expression signature. Full-blood counts from Healthy Donors (H.D.), Latent TB infected, active TB, and cured TB patients were performed. Frequency, phenotype, burst activity, and suppressor T cell activity of the two different subse…
Non-specific Effects of Vaccines Illustrated Through the BCG Example: From Observations to Demonstrations
2018
Epidemiological studies regarding many successful vaccines suggest that vaccination may lead to a reduction in child mortality and morbidity worldwide, on a grander scale than is attributable to protection against the specific target diseases of these vaccines. These non-specific effects (NSEs) of the Bacille Calmette-Guérin (BCG) vaccine, for instance, implicate adaptive and innate immune mechanisms, with recent evidence suggesting that trained immunity might be a key instrument at play. Collectively referring to the memory-like characteristics of innate immune cells, trained immunity stems from epigenetic reprogramming that these innate immune cells undergo following exposure to a primary…