Search results for "NAD"
showing 10 items of 2033 documents
Diseño y validación de un cuestionario para conocer la opinión del alumnado de secundaria sobre la Música Festera y sus aplicaciones en el aula
2016
El objetivo del presente estudio es presentar el diseno y la validacion de un cuestionario para conocer la opinion del alumnado de ensenanza secundaria de Torrellano (Alicante) sobre la Musica Festera y sus posibilidades de aplicacion en el aula de secundaria. La poblacion de referencia esta formada por 597 alumnos de musica distribuidos en cuatro cursos de ensenanza secundaria (1o, 2o, 3o y 4o). Si trabajamos con un nivel de confianza del 95% y un margen de error del 5%, el tamano que precisa la muestra de los alumnos para ser representativa de la poblacion de referencia es de 234. El cuestionario fue enviado a toda la poblacion y la muestra generadora de datos resulto ser de 484. La prime…
Familias reconstituidas: el cuestionamiento de los roles tradicionales parentales encarnados en las madres y las madrastras
2019
Investigating different migration patterns of Salmo trutta (L.) in the Teno river system using stable isotope analyses
2009
Emoticonos y emojis: análisis de su historia, difusión y uso en la comunicación digital actual
2016
Los emoticonos son unas populares representaciones esquemáticas de expresiones faciales o pequeñas imágenes que se añaden a los mensajes electrónicos. Tras una revisión la investigación sobre la comunicación mediada por ordenador (CMO) y los emoticonos, el trabajo recorre la historia de los emoticonos, las diferentes tipologías y discute su actual percepción y difusión. Desde el punto de vista empírico, el trabajo se basa en una encuesta exploratoria y en el análisis de un corpus. La encuesta fue respondida por una muestra de conveniencia de 226 personas. La encuesta abordó algunos aspectos poco estudiados en la investigación sobre emoticonos, como por ejemplo las herramientas en las que se…
Differenzierung zwischen prämaturer Thelarche und Pubertas praecox anhand klinischer, hormoneller und radiologischer Befunde
2008
In a retrospective study of 39 girls (aged 10 months to 7 10/12 years) with premature breast development criteria for distinguishing between premature thelarche and precocious puberty were analysed. Serum estradiol levels and bone age were determined and a test with luteinizing hormone-releasing hormone (LHRH) performed (inclusion criteria). On the basis of the LHRH test and bone age, premature thelarche was diagnosed in 29 patients and precocious puberty in ten: while those with premature thelarche had a follicle-stimulating hormone (FSH) pattern of rise, in those with precocious puberty the rise in gonadotropin was of the LH type. The LH/FSH ratio 30 min after stimulation was 1 (median 2.…
2002
Analysis of gonad organization and development revealed that Serranus atricauda is a functional simultaneous hermaphrodite. The ovary is classified as asynchronous. Oocyte growth is divided into five stages: stage I (primary growth stage), stage II (yolk vesicle formation), stage III (vitellogenesis), stage IV (oocyte maturation) and stage V (mature egg). The testis is of the unrestricted or lobular spermatogonial type. Spermatogenic cells include spermatogonia, primary and secondary spermatocytes, spermatids (which form cysts in the seminiferous tubules) and spermatozoa (free in the lumen). The histological structure of the gonad and sperm morphology would seem to indicate that this specie…
Isolation of Zebrafish Gonads for RNA Isolation
2013
Piwi proteins and piRNAs are abundant in the gonads of various animal species. Gonads from different developmental stages provide us information regarding the function of piRNAs and the PIWI pathway during germline development. Here we describe methods for gonad and germ cell preparation from different developmental stages of zebrafish. We also describe how to use these gonads to purify and characterize piRNAs.
Sugar moiety has a direct influence on the secondary structure and properties of sturgeon gonadotropin
2009
Sugar moiety has a direct influence on the secondary structure and properties of sturgeon gonadotropin Sturgeon Acipenser güldenstädti Br. gonadotropic hormone (GTH) β-subunit (β-GTH) was selectively chemically deglycosylated (dg) by anhydrous hydrogen fluoride and trifluoromethane sulfonic acid. Two dgβ-GTH molecular forms retaining 35% (dgβ-GTH1) and 13% (dgβ-GTH2) of their initial carbohydrates were obtained. Investigation of the reassociated α-β hybrid dimers (recombinants) α-GTH+dgβ-GTH1 and α-GTH+dgβ-GTH2 showed that the immunoreactivity with antiserum raised against standard GTH dropped by 22.5%. Hybrid dimers were recognised by the standard GTH antibodies, which indicated that the s…
Ontogeny of gonadotropin releasing hormone and gonadotropin immunoreactivity in brain and pituitary of normal and estrogen-treated guppies, Poecilia …
1987
Gonadotropin releasing hormone (GnRH) and gonadotropic hormone (GTH) were identified by immunohistochemistry in the brains and pituitaries of neonate, juvenile and adult guppies. GTH was present in some cells of the pars intermedia (pi) and proximal pars distalis (ppd) of all animals. GnRH was found in the perikarya of the nucleus olfactoretinalis. In the pituitaries of juvenile 30-day-old guppies, GnRH-immunoreactive cells existed in a "juvenile pattern", whereas in adult animals GnRH was recognized in only a few cells. GnRH-immunoreactive fibers were seen in the pituitaries of animals that were 30 days or older. In adult guppies, the ventral and lateral ppd (the gonadotropic region) conta…
Extensive nuclear gyration and pervasive non-genic transcription during primordial germ cell development in zebrafish.
2020
ABSTRACT Primordial germ cells (PGCs) are the precursors of germ cells, which migrate to the genital ridge during early development. Relatively little is known about PGCs after their migration. We studied this post-migratory stage using microscopy and sequencing techniques, and found that many PGC-specific genes, including genes known to induce PGC fate in the mouse, are only activated several days after migration. At this same time point, PGC nuclei become extremely gyrated, displaying general broad opening of chromatin and high levels of intergenic transcription. This is accompanied by changes in nuage morphology, expression of large loci (PGC-expressed non-coding RNA loci, PERLs) that ar…