Search results for "NGS"

IP6K gene identification in plant genomes by tag searching

2011

Abstract Background Plants have played a special role in inositol polyphosphate (IP) research since in plant seeds was discovered the first IP, the fully phosphorylated inositol ring of phytic acid (IP6). It is now known that phytic acid is further metabolized by the IP6 Kinases (IP6Ks) to generate IP containing pyro-phosphate moiety. The IP6K are evolutionary conserved enzymes identified in several mammalian, fungi and amoebae species. Although IP6K has not yet been identified in plant chromosomes, there are many clues suggesting its presences in vegetal cells. Results In this paper we propose a new approach to search for the plant IP6K gene, that lead to the identification in plant genome…

Poloxamer/sodium cholate co-formulation for micellar encapsulation of Doxorubicin with high efficiency for intracellular delivery: an in-vitro bioava…

2020

Abstract Hypothesis Doxorubicin hydrochloride (DX) is widely used as a chemotherapeutic agent, though its severe side-effects limit its clinical use. A way to overcome these limitations is to increase DX latency through encapsulation in suitable carriers. However, DX has a high solubility in water, hindering encapsulation. The formulation of DX with sodium cholate (NaC) will reduce aqueous solubility through charge neutralization and hydrophobic interactions thus facilitating DX encapsulation into poloxamer (F127) micelles, increasing drug latency. Experiments DX/NaC/PEO-PPO-PEO triblock copolymer (F127) formulations with high DX content (DX-PMs) have been prepared and characterized by scat…

Modification of halloysite lumen with dopamine derivatives as filler for antibiofilm coating

2023

Hypothesis: Development of nanocomposite coating with antibiofilm properties is of fundamental importance to efficient fight biofilm formation preventing infections in biomedical area. In this context, halloysite nanotubes (HNTs), biocompatible and low-cost clay mineral, have been efficiently used as filler for different polymeric matrices affording several nanocomposites with appealing antimicrobial properties. The modification of HNTs surfaces represents a valuable strategy to improve the utilization of the clay for biological purposes. Experiments: Herein, the covalent modification of the HNTs lumen with properly designed dopamine derivatives with different perfluoroalkyl chain length is…

Saving time when measuring BET isotherms.

2007

The measurement of adsorption isotherms and the detn. of surface properties of a solid by means of the BET equation usually takes a lot of time as it involves measurement of several dynamic adsorption curves each at a given gas pressure. Two different timesaving approaches are suggested: (a) the beginning of a single dynamic curve is considered and (b) the beginnings of a no. of different dynamic curves is considered. Approach (a) is less time consuming than approach (b), where approach (b) yields more accurate ests. of the BET-parameters. A discussion is given of the possibilities of using the time saving procedure of Jaentti for the two approaches. [on SciFinder (R)]

Surface modification of AISI-304 steel by ZnO synthesis using cathodic cage plasma deposition

2021

Abstract Zinc-oxide (ZnO), a solid lubricant coating, can increase the wear resistance of steels by working as a self-lubricant. In this study, ZnO film is synthesized using the cathodic cage plasma deposition (CCPD) technique, using galvanized steel cathodic cage (steel cage with zinc coating). The effect of gas composition (H2 is added in Ar-O2) is investigated to optimize the film properties. The surface hardness is increased more than twice in each processing condition. The deposited film shows ZnO phases for samples treated with low hydrogen contents and a combination of ZnO and magnetite phase (Fe3O4) with higher hydrogen contents. The thickness of film reduced from 1.28 μm to 0.5 μm …

Effects of hydrostatic pressure on the thermoelectric properties of the ϵ-polytype of InSe, GaSe, and InGaSe2 semiconductor compounds: anab initiostu…

2017

Recent developments and applications of smart nanoparticles in biomedicine

2022

Abstract Over the last decades, nanotechnology applied in medicine (nanomedicine) has sparked great interest from the scientific community, thanks to the possibility to engineer nanostructured materials, including nanoparticles (NPs), for a specific application. Their small size confers them unique properties because they are subject to physical laws in the middle between classical and quantum physics. This review is proposed to explain better how to design a specific NP and clarify the relationship between the type, size, and shape of NPs and the specific medical applications. NPs are classified into inorganic (metallic NPs, quantum dots, carbon-based nanostructures, mesoporous silica NPs)…

Spin qubits with electrically gated polyoxometalate molecules

2007

Spin qubits offer one of the most promising routes to the implementation of quantum computers. Very recent results in semiconductor quantum dots show that electrically-controlled gating schemes are particularly well-suited for the realization of a universal set of quantum logical gates. Scalability to a larger number of qubits, however, remains an issue for such semiconductor quantum dots. In contrast, a chemical bottom-up approach allows one to produce identical units in which localized spins represent the qubits. Molecular magnetism has produced a wide range of systems with tailored properties, but molecules permitting electrical gating have been lacking. Here we propose to use the polyox…

Manganese(II) complexes of scorpiand-like azamacrocycles as MnSOD mimics.

2011

Mn(II) complexes of scorpiand-type azamacrocycles constituted by a tretrazapyridinophane core appended with an ethylamino tail including 2- or 4-quinoline functionalities show very appealing in vitro SOD activity. The observed behaviour is related to structural and electrochemical parameters.

Hybrid Quantum Mechanics/Molecular Mechanics Simulations with Two-Dimensional Interpolated Corrections:  Application to Enzymatic Processes

2006

Hybrid quantum mechanics/molecular mechanics (QM/MM) techniques are widely used to study chemical reactions in large systems. Because of the computational cost associated with the high dimensionality of these systems, the quantum description is usually restricted to low-level methods, such as semiempirical Hamiltonians. In some cases, the description obtained at this computational level is quite poor and corrections must be considered. We here propose a simple but efficient way to include higher-level corrections to be used in potential energy surface explorations and in the calculation of potentials of mean force. We evaluate a correction energy term as the difference between a high-level …