Search results for "NOE"
showing 10 items of 825 documents
The Candida albicans cell wall-associated glyceraldehyde-3-phosphate dehydrogenase activity increases in response to starvation and temperature upshi…
2002
We have determined the effect of environmental factors (mild thermal upshift and starvation) on the Candida albicans cell wall-associated glyceraldehyde-3-phosphate dehydrogenase (cwGAPDH) activity. Temperature upshift (from 28 to 37 degrees C) and/ or starvation (at 28 or 37 degrees C in water) of exponentially growing yeast cells caused an increase in cwGAPDH activity (3 to 5-, and 7 to 8-fold, respectively). This increase in activity did not correlate with an increase in the amount of cwGAPDH protein present, as determined by flow cytometry, immunoelectron microscopy and Western-blotting. These results indicate that thermal upshift and starvation cause an activation of the cwGAPDH in C. …
Immunoelectron Microscopy of Hemocyanin from the Keyhole Limpet (Megathura crenulata): A Parallel Subunit Model
1993
Abstract Immunoelectron microscopy has been performed using negatively stained immune complexes of keyhole limpet hemocyanin (KLH) subunit 2 di- and multidecamers with domain-specific monoclonal antibodies. One antibody (KLH2 a macr 1) links the hemocyanin molecules in a side-to-side pattern, whereas the other antibody (KLH2 fg macr 1) links the molecules end-to-end. From existing knowledge of the domain sequence of KLH subunit 2, these data provide support for a parallel arrangement of subunits within each decamer. Ten N-terminal a macr: domains are then present at the noncollar region of each decamer with 10 C-terminal g macr domains at the collar region. The immunonegative staining data …
Biopolymer-Based Hybrid Hydrogels for Biomedical Applications: From Macroscopic to Nanosized Systems
2021
Les partícules portadores basades en biopolímers són biocompatibles i ofereixen la capacitat d’incorporar agents actius hidròfils i hidròfobs. La incorporació d’un fàrmac en una matriu polimèrica pot millorar-ne la protecció contra la degradació en mitjans fisiològics severs. A més, pot controlar-ne l’alliberament al lloc d’acció específic, amb una biodisponibilitat terapèutica augmentada i efectes secundaris o toxicitat minimitzats. Aquesta tesi presenta la preparació de diferents sistemes híbrids de polisacàrids per a encapsular substàncies hidròfiles, que posteriorment són alliberades. Es preparen portadors que varien de mida macroscòpica a nanomètrica mitjançant diferents mètodes: gelif…
Use of Early Juvenile Zebrafish Danio Rerio for In-Vivo Assessment of Endocrine Modulation by Xenoestrogens
2013
Reliable and cost-effective early-life stage (ELS) bioassays incorporating practical experimentation without compromising scientific relevance are crucial in chemical risk assessment. This study investigated the use of 20 days- post-fertilization life stage ( 20dpfZF ) of zebrafish Danio rerio to screen environmental chemicals known to be estrogenic in adult fish. Firstly, studies with key genes in steroidogenesis were conducted; the brain isoform of aromatase gene ( cyp19a1b ) being the most prominently expressed biomarker. Regulation of mRNA levels of molecular biomarkers, vitellogenin 1 gene ( vtg1) and cyp19a1b were selected to assess the endocrine modulation by xenoestrogens, 17α-ethin…
CCDC 789094: Experimental Crystal Structure Determination
2011
Related Article: S.Naiya, C.Biswas, M.G.B.Drew, C.J.Gomez-Garcia, J.M.Clemente-Juan, A.Ghosh|2010|Inorg.Chem.|49|6616|doi:10.1021/ic1005456
CCDC 202460: Experimental Crystal Structure Determination
2005
Related Article: H.Fleischer, D.Schollmeyer|2004|Inorg.Chem.|43|5529|doi:10.1021/ic0498889
Quadruply-bonded dimolybdenum compounds: Reactivity towards TCNE. Structural evidences for the 1-D polymer [Mo2(O2 CCF3)4(TCNE)]∞
2005
cited By 3; International audience; Reaction of [Mo2(O2CR)4] (R = CF 3, 1) with TCNE in toluene affords the new compound [Mo2(O2CCF3)4 (TCNE)·6H5CH3 (2). The structure of 2 is built on [Mo2(O2 CCF3)4] fragments having the usual paddlewheel structure of 1 (Mo-Mo 2.1117(8) Å) and TCNE units. Each polynitrile moiety acts as a bridging ligand between two Mo2 fragments (Mo-N 2.875(4) Å) affording 1-D polymeric chains crossing in the crystal; the toluene molecules occupy the cavities between the chains. 13C NMR, IR and electrochemical data clearly show that formation of 2 does not involve electron density transfer from dimolybdenum fragment to the TCNE unit, which remains in 2 in its neutral stat…
Sponge aggregation factor: identification of the specific collagen-binding site by means of a monoclonal antibody.
1988
The aggregation factor (AF) from the sponge Geodia cydonium is known to be a complex proteinaceous particle, composed of a series of different (glyco)proteins (Mr lower than 150,000) around a 90S sunburst-like core structure. One of the low-Mr proteins is the 47-KD cell binding fragment. We describe a new monoclonal antibody (mAb), III1E6, raised against purified AF particles, which recognizes in tissue slices structures present both on the plasma membrane and in a network-like manner in the extracellular space. By applying immunoelectron microscopical, immunoblotting, and immunoaffinity chromatographical techniques, the mAb III1E6 was shown to recognize the core structure of the AF partic…
A molecular electron density theory study of the participation of tetrazines in aza-Diels–Alder reactions
2020
The reactions of eight tetrazines of increased electrophilic character with nucleophilic tetramethyl ethylene (TME) and with electrophilic tetracyanoethylene (TCE) have been studied using Molecular Electron Density Theory. These reactions are domino processes comprising an aza-Diels–Alder (ADA) reaction followed by an extrusion of molecular nitrogen, yielding a dihydropyridazine. Analysis of the conceptual DFT (CDFT) indices showed an increase of the electrophilicity and a decrease of the nucleophilicity of tetrazines with an increase of the electron-withdrawing character of the substituent. A very good correlation between the global electron density transfer at the transition structu…
Comparative study for separation of aquatic humic-type organic constituents by DAX-8, PVP and DEAE sorbing solids and tangential ultrafiltration: ele…
2005
Aquatic humic-type solutes were separated in parallel from the same fresh water source by four different procedures: non-ionic polymethyl methacrylate (DAX-8) and functional cross-linked polyvinylpyrrolidone (PVP) resins, functional diethylaminoethyl cellulose (DEAE) and tangential ultrafiltration completed with a weakly basic anion exchange resin (IRA-67). The similarity-dissimilarity between the quantities and qualities of the different humic samples is discussed, especially in the light of the original dissolved organic matter (DOM). During the past two decades, a significant progress has occurred in the aquatic humic research due to the so-called hydrophobic-hydrophilic properties posse…