Search results for "Nanolithography"
showing 10 items of 62 documents
Surface Lattice Resonances in Self-Assembled Arrays of Monodisperse Ag Cuboctahedra
2019
Plasmonic metal nanoparticles arranged in periodic arrays can generate surface lattice plasmon resonances (SLRs) with high Q-factors. These collective resonances are interesting because the associated electromagnetic field is delocalized throughout the plane of the array, enabling applications such as biosensing and nanolasing. In most cases such periodic nanostructures are created via top-down nanofabrication processes. Here we describe a capillary-force-assisted particle assembly method (CAPA) to assemble monodisperse single-crystal colloidal Ag cuboctahedra into nearly defect-free >1 cm2 hexagonal lattices. These arrays are large enough to be measured with conventional ultraviolet-visibl…
Dielectrophoretic trapping of multilayer DNA origami nanostructures and DNA origami-induced local destruction of silicon dioxide
2015
DNA origami is a widely used method for fabrication of custom-shaped nanostructures. However, to utilize such structures, one needs to controllably position them on nanoscale. Here we demonstrate how different types of 3D scaffolded multilayer origamis can be accurately anchored to lithographically fabricated nanoelectrodes on a silicon dioxide substrate by DEP. Straight brick-like origami structures, constructed both in square (SQL) and honeycomb lattices, as well as curved "C"-shaped and angular "L"-shaped origamis were trapped with nanoscale precision and single-structure accuracy. We show that the positioning and immobilization of all these structures can be realized with or without thi…
One-step large-scale deposition of salt-free DNA origami nanostructures
2015
AbstractDNA origami nanostructures have tremendous potential to serve as versatile platforms in self-assembly -based nanofabrication and in highly parallel nanoscale patterning. However, uniform deposition and reliable anchoring of DNA nanostructures often requires specific conditions, such as pre-treatment of the chosen substrate or a fine-tuned salt concentration for the deposition buffer. In addition, currently available deposition techniques are suitable merely for small scales. In this article, we exploit a spray-coating technique in order to resolve the aforementioned issues in the deposition of different 2D and 3D DNA origami nanostructures. We show that purified DNA origamis can be …
Nanofabrication of TaS2 conducting layers nanopatterned with Ta2O5 insulating regions via AFM
2013
It is demonstrated how local oxidation nanolithography performed with an atomic force microscope (AFM-LON) may be successfully employed for the nanopatterning of insulating regions of Ta2O5 on TaS2 ultrathin metallic layers. This provides a simple approach for the fabrication of electronic devices, such as single-electron transistors, at the nanoscale.
Large-scale Nanopatterning of Single Proteins used as Carriers of Magnetic Nanoparticles
2010
4 páginas, 4 figuras.
DNA Origami-Mediated Substrate Nanopatterning of Inorganic Structures for Sensing Applications
2019
Structural DNA nanotechnology provides a viable route for building from the bottom-up using DNA as construction material. The most common DNA nanofabrication technique is called DNA origami, and it allows high-throughput synthesis of accurate and highly versatile structures with nanometer-level precision. Here, it is shown how the spatial information of DNA origami can be transferred to metallic nanostructures by combining the bottom-up DNA origami with the conventionally used top-down lithography approaches. This allows fabrication of billions of tiny nanostructures in one step onto selected substrates. The method is demonstrated using bowtie DNA origami to create metallic bowtie-shaped an…
Biomimetic routes to micro/nanofabrication
2020
Abstract The discovery of inorganic polyphosphate (polyP), an evolutionarily ancient biopolymer, and of its function in energy storage/supply and induction of cell differentiation through specific gene expression (“morphogenetic activity”) has opened new opportunities in regenerative medicine, including therapy of bone, cartilage, and vascular diseases. Inspired by nature, various procedures have been developed for the preparation of amorphous nano and microparticles of polyP with different counterions, which mimic the physiological polyP found in blood platelets/acidocalcisomes, as well as for the preparation of morphogenetically active polyP coacervate complexes. This chapter summarizes t…
A Protein-Interaction Array Inside a Living Cell
2013
Cell phenotype is determined by protein network states that are maintained by the dynamics of multiple protein interactions.1 Fluorescence microscopy approaches that measure protein interactions in individual cells, such as by Forster resonant energy transfer (FRET), are limited by the spectral separation of fluorophores and thus are most suitable to analyze a single protein interaction in a given cell. However, analysis of correlations between multiple protein interactions is required to uncover the interdependence of protein reactions in dynamic signal networks. Available protein-array technologies enable the parallel analysis of interacting proteins from cell extracts, however, they can …
Printing Biology for Advanced Synthetic Biosystem
2019
Printing technologies represent a powerful tool for the direct micro- and nano- fabrication of biomolecular structures at the interface between life and materials sciences (Arrabito et al., 2012). Their continuous development over the last years has permitted the onset of man-made biosystems with customizable dimensions (from the micron-scale down to the nanometer scale), composition (organic molecules, DNA, proteins, phospholipids), and relevant functions (molecular interactions, drug screening, cellular biointerfaces, cell-like compartments). In this work, we show the possibility to leverage the fabrication of a wide class of solid-supported or liquid-liquid based synthetic compartments b…
Micro and Nano patterns for Biosensing: from enzymatic assays to single cells interaction arrays
2012
In this thesis work, solution dispensing techniques have been employed for the realization of complex biological arrays. Inkjet printing techniques were employed for the generation of drug screening platforms. This approach was initially proved with a model enzyme system like Glucose Oxidase substrate covalently linked to a functionalized silicon oxide support. On this support an enzymatic substrate (D-glucose)/inhibitor (D-glucal) couple was accurately dispensed. A simple optical detection method was used to prove the screening capability of the microarray with the possibility to assay with high reproducibility at the single spot level. Afterwards, this methodology has been extended to CYP…