Search results for "Negative"
showing 10 items of 1052 documents
E-wave and heart rate responses during anticipation of nonmotor events.
1994
This study concentrated on three main questions: 1) can anticipatory late negative shift (expectancy wave, E-wave) be elicited in nonmotor S1-S2 paradigm, 2) is it sensitive to variation of emotional aspects of the task and 3) is there a connection between heart rate (HR) responses and E-wave. S1 was a letter row that was replaced tachistoscopically by another letter row (S2). The task of the subjects (n = 12) was to detect if the critical aspects of S2 were similar to S1. After their delayed response they received feedback of their performance. The emotional aspects of the task were varied by presenting aversive noise bursts at the end of the feedback period either always, contingently to …
Predictors of Finnish Adolescent’s Prejudice towards Russian Immigrants and the Effect of Intergroup Contact
2016
This study examined perceived threat as a predictor of Finnish adolescent’s prejudice towards Russian immigrants. Moreover, since Russian immigrants represent the largest immigrant group in Eastern Finland, this study also explored the relationship between intergroup contact, threat, and prejudice. The sample consisted of 305 Finnish adolescents ranging from 11 to 19 years old. Results showed threat to be a significant predictor of prejudice towards Russian immigrants in Eastern Finland. Individually, negative stereotype was found to be the only threat that significantly predicted prejudice towards Russian immigrants. Realistic and symbolic threats were not important to the attitudes of Fin…
Perceived threat and prejudice towards immigrants in Finland : A study among early, middle, and late Finnish adolescents
2017
ABSTRACTUsing integrated threat theory, this study examined how perceived threat, or fear of immigrants, manifests among early, middle, and late Finnish adolescents, and the relationship between perceived threat and prejudice among early, middle, and late adolescents. The sample consisted of 795 Finnish adolescents between 11 and 19 years of age. Realistic and symbolic threats were the most perceived threats and were more prevalent among late adolescents. There was a positive relationship between prejudice and realistic threat, and between prejudice and symbolic threat, but a negative relationship between prejudice and negative stereotyping, and this relationship remained relatively stable …
How Are Negative Customer Experiences Formed? A Qualitative Study of Customers’ Online Shopping Journeys
2019
This study investigates how negative customer experiences are formed during customers’ online shopping journeys. A qualitative, in-depth dataset collected from 34 participants was employed to identify negatively perceived touchpoints that contribute to the customer experience in a negative way. The findings reveal that negative touchpoints are experienced during customers’ entire journeys, particularly after a purchase is completed. We identified 152 negative touchpoints from the data, of which 53 were experienced during search and consideration, 35 when finalizing a purchase, 33 during delivery, and 31 during after-sales interactions with the company. Within these four main categories, 20 …
ICTV Virus Taxonomy Profile: Cystoviridae
2017
The family Cystoviridae includes enveloped viruses with a tri-segmented dsRNA genome and a double-layered protein capsid. The innermost protein shell is a polymerase complex responsible for genome packaging, replication and transcription. Cystoviruses infect Gram-negative bacteria, primarily plant-pathogenic Pseudomonas syringae strains. This is a summary of the International Committee on Taxonomy of Viruses (ICTV) Report on the taxonomy of the Cystoviridae, which is available at http://www.ictv.global/report/cystoviridae.
A loop involving NRF2, miR‐29b‐1‐5p and AKT, regulates cell fate of MDA‐MB‐231 triple‐negative breast cancer cells
2019
The present study shows that nuclear factor erythroid 2-related factor 2 (NRF2) and miR-29b-1-5p are two opposite forces which could regulate the fate of MDA-MB-231 cells, the most studied triple-negative breast cancer (TNBC) cell line. We show that NRF2 activation stimulates cell growth and markedly reduces reactive oxygen species (ROS) generation, whereas miR-29b-1-5p overexpression increases ROS generation and reduces cell proliferation. Moreover, NRF2 downregulates miR-29b-1-5p expression, whereas miR-29b-1-5p overexpression decreases p-AKT and p-NRF2. Furthermore, miR-29b-1-5p overexpression induces both inhibition of DNA N-methyltransferases (DNMT1, DNMT3A, and DNMT3B) expression and …
Mechanisms of quinolone resistance in Aeromonas species isolated from humans, water and eels.
2009
Mechanisms of resistance were determined in 33 quinolone-resistant isolates of the species Aeromonas hydrophila, Aeromonas caviae, Aeromonas media, Aeromonas salmonicida, Aeromonas popoffii and Aeromonas veronii, recovered from humans, freshwater and eels. The quinolone resistance-determining regions (QRDRs) of gyrA and parC genes were sequenced in these resistant strains, as well as in 8 quinolone-sensitive Aeromonas used as controls. All quinolone-resistant Aeromonas carried point mutations in the gyrA QRDR at codon 83, respectively giving rise to substitutions Ser(83)-->Ile (32 strains) or Ser(83)-->Val (1 strain). Almost half of these isolates (48%) carried additional point mutations in…
Evaluation of the DNA microarray “AMR Direct Flow Chip Kit” for detection of antimicrobial resistance genes from Gram-positive and Gram-negative bact…
2019
Abstract Introduction The AMR Direct Flow Chip assay allows the simultaneous detection of a large variety of antibiotic resistance genetic markers. To assess this kit's performance, we use isolated colonies as starting material. The assay has been approved by the European Economic Area as a suitable device for in vitro diagnosis (CE IVD) using clinical specimens. Methods A total of 210 bacterial isolates harbouring either one or more antimicrobial resistance genes including plasmid-encoded extended-spectrum β-lactamases (SHV, CTX-M) and carbapenemases (GES, SME, KPC, NMC/IMI, SIM, GIM, SPM, NDM, VIM, IMP, and OXA), mecA, vanA and vanB, and 30 controls were included. Results The assay displa…
Freezing Induces Biased Results in the Molecular Detection of Flavobacterium columnare
2006
ABSTRACT Specific PCR detection and electron microscopy of Flavobacterium columnare revealed the risk of false-negative results in molecular detection of this fish pathogen. Freezing and thawing destroyed the cells so that DNA was for the most part undetectable by PCR. The detection of bacteria was also weakened after prolonged enrichment cultivation of samples from infected fish.
Detection and organization of atrazine-degrading genetic potential of seventeen bacterial isolates belonging to divergent taxa indicate a recent comm…
2007
A collection of 17 atrazine-degrading bacteria isolated from soils was studied to determine the composition of the atrazine-degrading genetic potential (i.e. trzN, trzD and atz) and the presence of IS1071. The characterization of seven new atrazine-degrading bacteria revealed for the first time the trzN-atzBC gene composition in Gram-negative bacteria such as Sinorhizobium sp. or Polaromonas sp. Three main atrazine-degrading gene combinations (i) trzN– atzBC, (ii) atzABC– trzD and (iii) atzABCDEF were observed. The atz and trz genes were often located on plasmids, suggesting that plasmid conjugation could play an important role in their dispersion. In addition, the observation of these gene…