Search results for "Nitrification"
showing 10 items of 192 documents
Biological minimization of excess sludge in a membrane bioreactor: Effect of plant configuration on sludge production, nutrient removal efficiency an…
2018
Abstract Excess sludge minimization was studied in a MBR with pre-denitrification scheme. Sludge minimization, nitrogen removal performance and membrane fouling tendency were investigated in two configurations, characterized by a different position of the sludge retention reactor (SRR). In particular, the SRR was placed: i) in the return activated sludge line (Anaerobic Side-Stream Reactor – ASSR configuration) and ii) in the mainstream between the anoxic and aerobic reactor (Anaerobic Main-Stream Reactor – AMSR configuration). The achieved results demonstrated that the ASSR enabled a higher excess sludge reduction (74% vs 32%), while achieving lower biological nitrogen removal (BNR) (TN = …
A comprehensive integrated membrane bioreactor model for greenhouse gas emissions
2018
Abstract A comprehensive integrated membrane bioreactor (MBR) model for wastewater treatment is here proposed. The model quantifies the main biological and physical processes. The model describes the biological removal of organic matter, nitrogen and phosphorus including greenhouse gases (carbon dioxide, CO 2 and nitrous oxide, N 2 O). The model takes into account the following main innovative aspects jointly: i. Two-step nitrification process; ii. N 2 O formation due to ammonia-oxidizing bacteria as a product of the hydroxylamine oxidation (NH 2 OH) and of the nitrite (NO 2 − ) reduction; iii. Soluble microbial product (SMP) formation/degradation due to microbial growth and endogenous resp…
Membrane bioreactors for treatment of saline wastewater contaminated by hydrocarbons (diesel fuel): An experimental pilot plant case study
2016
Abstract The paper reports the main results of an experimental campaign performed on a membrane bioreactor pilot plant designed to treat synthetic shipboard slops. The experimental campaign was divided into two phases: salinity acclimation up to 20 g NaCl L −1 (Phase I) and hydrocarbon (diesel fuel) dosing (Phase II). The observed results show that the carbon removal was not severely affected by the wastewater features. Conversely, respirometric tests showed that nitrification was strongly affected by the salinity (33% of nitrification efficiency at 20 g NaCl L −1 – Phase I) as a result of the salinity in the autotrophic biomass. Moreover, the sludge viscosity increased during Phase II due …
Sequential batch membrane bio-reactor for wastewater treatment: The effect of increased salinity
2016
In this work, a sequential batch membrane bioreactor pilot plant is investigated to analyze the effect of a gradual increase in salinity on carbon and nutrient removal, membrane fouling and biomass kinetic parameters. The salinity was increased by 2 g NaCl L-1 per week up to 10 g NaCl L-1. The total COD removal efficiency was quite high (93%) throughout the experiment. A gradual biomass acclimation to the salinity level was observed during the experiment, highlighting the good recovery capabilities of the system. Nitrification was also influenced by the increase in salinity, with a slight decrease in nitrification efficiency (the lowest value was obtained at 10 g NaCl L-1 due to lower nitri…
Treatment of Oily Wastewater with Membrane Bioreactor Systems
2017
The aim of the present work was to investigate the behavior of a membrane bioreactor (MBR) system for the treatment of oily wastewater. A bench scale MBR was fed with synthetic wastewater containing diesel fuel. Organic carbon, hydrocarbon and ammonium removal, kinetic constants, extracellular polymeric substances production, and membrane fouling rates were monitored. The MBR plant was operated for more than 200 days, and the results highlighted good carbon removal and nitrification, suggesting a sort of biomass adaptation to hydrocarbons. Membrane fouling analysis showed an increase in total resistance, likely due to hydrocarbons, which caused an irreversible fouling (pore blocking) mainly…
Selective enrichment of heterotrophic nitrifiers <em>Alcaligenaceae</em> and <em>Alcanivorax</em> spp. from industrial wastew…
2020
Removal of nitrogen from wastewaters (WW) represents a global problem. The low nitrification rate during WW treatment is often caused by ecotoxicity. This problem is attributed mostly to the industrial WW. Our study was focused on the testing of industrial WW and activated sludge (AS) with the aim to reveal the abundance of nitrifiers and increase their biomass, thus, providing the additional step, i.e., bioaugmentation, within the technological process of WW treatment. Plating of AS on the selective solidified media designated for the 1st and 2nd nitrification stages, resulted in the shift in bacterial community structure with dominated Alcaligenaceae and Alcanivorax for the 1st stage, and…
Quantification of denitrifying bacteria in soils by nirK gene targeted real-time PCR.
2004
Abstract Denitrification, the reduction of nitrate to nitrous oxide or dinitrogen, is the major biological mechanism by which fixed nitrogen returns to the atmosphere from soil and water. Microorganisms capable of denitrification are widely distributed in the environment but little is known about their abundance since quantification is performed using fastidious and time-consuming MPN-based approaches. We used real-time PCR to quantify the denitrifying nitrite reductase gene (nirK), a key enzyme of the denitrifying pathway catalyzing the reduction of soluble nitrogen oxide to gaseous form. The real-time PCR assay was linear over 7 orders of magnitude and sensitive down to 102 copies by assa…
Denitrification in pathogenic bacteria : for better or worst ?
2005
A large variety of physiological and taxonomic groups have the ability to use nitrogen oxides as alternative electron acceptors. Brucella spp. is an alpha-proteobacteriaceae that induces a persistent disease in some mammals. Recent work has revealed that a denitrifying gene cluster is important in the interaction of Brucella neotomoae with its host.
Functional gene pyrosequencing reveals core proteobacterial denitrifiers in boreal lakes
2015
Denitrification is an important microbial process in aquatic ecosystems that can reduce the effects of eutrophication. Here, quantification and pyrosequencing of nirS, nirK, and nosZ genes encoding for nitrite and nitrous oxide reductases was performed in sediment samples from four boreal lakes to determine the structure and seasonal stability of denitrifying microbial populations. Sediment quality and nitrate concentrations were linked to the quantity and diversity of denitrification genes, the abundance of denitrifying populations (nirS and nosZ genes) correlated with coupled nitrificationdenitrification (Dn), and the denitrification of the overlying water NO3 − (Dw) correlated with the n…
Local response of bacterial densities and enzyme activities to elevated atmospheric CO2 and different N supply in the rhizosphere of Phaseolus vulgar…
2008
Publication Inra prise en compte dans l'analyse bibliométrique des publications scientifiques mondiales sur les Fruits, les Légumes et la Pomme de terre. Période 2000-2012. http://prodinra.inra.fr/record/256699; Altered flux of labile C from plant roots into soil is thought to influence growth and maintenance of microbial communities under elevated atmospheric CO2 concentrations. We studied the abundance and function of the soil microbial community at two levels of spatial resolution to assess the response of microorganisms in the rhizosphere of the whole root system and of apical root zones of Phaseolus vulgaris L. to elevated CO2 and high or low N supply. At the coarser resolution, microb…