Search results for "Node"
showing 10 items of 1701 documents
The role of cell interactions in the control of RNA synthesis.
1967
Cutting edge: priming of CTL by transcutaneous peptide immunization with imiquimod.
2005
Abstract CTL are important in combating cancer and viruses. Therefore, triggering the complete potential of CTL effector functions by new vaccination strategies will not only improve prophylaxis of tumor or virus-related diseases, but also open opportunities for effective therapeutic immunizations. Using transcutaneous immunization, we show that epicutaneous (e.c.)4 application of an ointment containing a CTL epitope and the TLR7 ligand imiquimod is highly effective in activating T cells in mice using TCR-transgenic CTL or in wild-type mice. Transcutaneous immunization-activated CTL mount a full-blown immune response against the target epitope characterized by proliferation, cytolytic activ…
The hemolysin-producer coelomocytes in Holothuria polii
1988
Using sodium metrizoate discontinuous gradients, two hemolysin-producer amebocyte populations have been separated from total circulating Holothuria polii coelomocytes. The amebocytes of population 1 are responsible for the production of the calcium-dependent and temperature-labile hemolysin, whereas those of population 2 produce the calcium-independent and temperature-stable one. The intracytoplasmic hemolysins were evidenced also by immunofluorescence. Petaloid and filipodial amebocytes were the only positive cell types.
Both mature KIR+ and immature KIR- NK cells control pediatric acute B-cell precursor leukemia in NOD.Cg-Prkdcscid IL2rgtmWjl/Sz mice.
2014
Therapeutic natural killer (NK)-cell-mediated alloreactivity toward acute myeloid leukemia has largely been attributed to mismatches between killer immunoglobulin-like receptors (KIRs) on NK cells and their ligands, HLA class I molecules, on target cells. While adult acute B-cell precursor leukemia (BCP-ALL) appears to be resistant to NK-cell-mediated lysis, recent data indicate that pediatric BCP-ALL might yet be a target of NK cells. In this study, we demonstrate in a donor-patient-specific NOD.Cg-Prkdc(scid) IL2rg(tmWjl)/Sz (NSG) xenotransplantation model that NK cells mediate considerable alloreactivity toward pediatric BCP-ALL in vivo. Notably, both adoptively transferred mature KIR(+)…
Impaired Mast Cell-Driven Immune Responses in Mice Lacking the Transcription Factor NFATc2
2009
Abstract The three calcium-dependent factors NFATc1, c2, and c3 are expressed in cells of the immune system and play pivotal roles in modulating cellular activation. With regard to NFATc2, it was reported that NFATc2-deficient mice display increased immune responses in several models for infection and allergy in vivo. This led to the assumption that NFATc2 is involved in the maintenance of immune homeostasis. Using the synthetic TLR7 agonist imiquimod as an adjuvant in epicutaneous peptide immunization, we observed that both the inflammatory reaction and the peptide-specific CTL response are severely impaired in NFATc2-deficient mice. Detailed analyses revealed that early production of proi…
DNA-Based Enzyme Reactors and Systems
2016
During recent years, the possibility to create custom biocompatible nanoshapes using DNA as a building material has rapidly emerged. Further, these rationally designed DNA structures could be exploited in positioning pivotal molecules, such as enzymes, with nanometer-level precision. This feature could be used in the fabrication of artificial biochemical machinery that is able to mimic the complex reactions found in living cells. Currently, DNA-enzyme hybrids can be used to control (multi-enzyme) cascade reactions and to regulate the enzyme functions and the reaction pathways. Moreover, sophisticated DNA structures can be utilized in encapsulating active enzymes and delivering the molecular…
Effects of HIV, antiretroviral therapy and prebiotics on the active fraction of the gut microbiota
2018
In a recent blinded randomized study, we found that in HIV-infected individuals a short supplementation with prebiotics (scGOS/lcFOS/glutamine) ameliorates dysbiosis of total gut bacteria, particularly among viremic untreated patients. Our study goal was to determine the fraction of the microbiota that becomes active during the intervention and that could provide additional functional information.A total of six healthy individuals, and 16 HIV-infected patients comprising viremic untreated patients (n = 5) and antiretroviral therapy-treated patients that are further divided into immunological responders (n = 7) and immunological nonresponders (n = 4) completed the 6-week course of prebiotic …
Can perfusion sensitized MR diffusion weighted imaging discriminate between benign and malignant nodes for patients with head and neck squamous cell …
2012
Objective: To investigate fast (perfusion sensitized) and slow (true diffusion sensitized) apparent diffusion coefficient (ADC) measurements as discriminators of benign from malignant lymph nodes in patients with head and neck (H&N) squamous cell carcinoma (SCC). Materials and Methods: Seventeen patients with H&N SCC staged for nodal disease using anatomical MRI, contrast enhanced CT and ultrasound ± fine needle aspiration gave informed consent for additional MR diffusion weighted imaging (DWI). Axial DWI was performed by short tau inversion recovery (STIR) echo planar imaging and trace weighted images obtained b 0, 50, 100, 300, 600 and 1000. Apparent diffusion coefficient (ADC) was calcul…
Knowledge Extraction from Environmental Data Through a Cognitive Architecture
2008
Wireless Sensor Networks represent a novel technology which is expected to experience a dramatic diffusion thanks to the promise to be a pervasive sensory means; however, one of the issues limiting their potential growth relies in the difficulty of managing and interpreting huge amounts of collected data. This paper proposes a cognitive architecture for the extraction of high-level knowledge from raw data through the representation of processed data in opportune conceptual spaces. The presented framework interposes a conceptual layer between the subsymbolic one, devoted to sensory data processing, and the symbolic one, aimed at describing the environment by means of a high level language. T…
Latvijā piedāvāto filmu izsekošanas tīmekļa lietotne “MovieTrack”
2018
Kvalifikācijas darbā izstrādāta “Latvijā piedāvāto filmu izsekošanas tīmekļa lietotne “MovieTrack””. “MovieTrack” ir tīmekļa lietotne, kuras pamatā ir kinoteātru datu savākšana, apvienošana, apstrāde un attēlošana. Tās mērķis ir sniegt lietotājam informāciju par aktuālajām filmām Latvijā un to seansiem dažādos kinoteātros. Kvalifikācijas darbs tika izstrādāts vadoties pēc spējās izstrādes metodes. Darba pirmkods un priekšgalsistēma tika realizēta, izmantojot Javascript ietvaru SAPUI5, bet aizmugursistēmas (back-end) daļa tika realizēta, izmantojot Node.js un MongoDB datubāzi.