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showing 10 items of 3963 documents
ATR-Spin: an open-source 3D printed device for direct cytocentrifugation onto attenuated total reflectance crystals.
2021
Infrared Spectroscopy (IR) enables the direct and rapid characterization of cells at the molecular level. Achieving a rapid and consistent cell preparation is critical for the development of Point-of-Care diagnostics for cell analysis. Here we introduce an open-source, 3D printed device for integrating the isolation, preconcentration, and measurement of attenuated total reflectance IR spectra of cells from biofluids. The tool comprises a disposable card for cytocentrifugation, equipped with magnets, which allows reproducible integration into the pathlength of the IR spectrophotometer. Preliminary results using cell media containing A459 cells indicate that this system enables a qualitative …
Genomic structure and functional characterization of the human ADAM10 promoter
2005
The ADAM10 gene encodes a membrane-bound disintegrin-metalloproteinase, which, after overexpression in an Alzheimer disease (AD) mouse model, prevents amyloid pathology and improves long-term potentiation and memory. Because enhancing ADAM10 expression appears to be a reasonable approach for treatment of AD, we functionally analyzed the ADAM10 gene. Both human and mouse ADAM10 genes comprise approximately 160 kbp, are composed of 16 exons, and are evolutionarily highly conserved within 500 bp upstream of either translation initiation site. By using luciferase reporter assays, we demonstrate that nucleotides -2179 to -1 upstream of the human ADAM10 translation initiation site represent a fun…
Small Residues Inhibit Homo-Dimerization of the Human Carbonic Anhydrase XII Transmembrane Domain
2021
Amino acids with small side chains and motifs of small residues in a distance of four are rather abundant in human single-span transmembrane helices. While interaction of such helices appears to be common, the role of the small residues in mediating and/or stabilizing transmembrane helix oligomers remains mostly elusive. Yet, the mere existence of (small)xxx(small) motifs in transmembrane helices is frequently used to model dimeric TM helix structures. The single transmembrane helix of the human carbonic anhydrases XII contains a large number of amino acids with small side chains, and critical involvement of these small amino acids in dimerization of the transmembrane domain has been sugges…
CCDC 1950443: Experimental Crystal Structure Determination
2021
Related Article: Liu-Pan Yang, Li Zhang, Mao Quan, Jas S. Ward, Yan-Long Ma, Hang Zhou, Kari Rissanen, Wei Jiang|2020|Nat.Commun.|11|2740|doi:10.1038/s41467-020-16534-9
CCDC 1913148: Experimental Crystal Structure Determination
2019
Related Article: Hongxin Chai, Zhi-Sheng Pan, Liu-Pan Yang, Shan He, Fangfang Pan, Kari Rissanen, Wei Jiang|2019|Chem.Commun.|55|7768|doi:10.1039/C9CC03341F
Unexpected Behavior of Enaminones: Interesting New Routes to 1,6-Naphthyridines, 2-Oxopyrrolidines and Pyrano[4,3,2-de][1,6]naphthyridines
2012
Reaction of enaminones 1a–d with 2-aminoprop-1-ene-1,1,3-tricarbonitrile (2) in the presence of AcOH/NH4OAc afforded 7-amino-5-oxo-5,6-dihydro-1,6-naphthyridine-8-carbonitrile derivatives 9a–d. On the other hand, 2-aminopyrano[4,3,2-de] [1,6]naphthyridine-3-carbonitriles 20a–c,e were the only obtained products from the reactions of 1a–d with 2 in the presence of AcOH/NaOAc, while 1d afforded [3,5-bis-(4-chloro-benzoyl)-phenyl]-(4-chloro-phenyl)-methanone 21 under the same condition. The reaction of 2 with diethyl acetylenedicarboxylate in the presence of AcOH/NH4OAc afforded (4-cyano-5-dicyanomethylene-2-oxo-2,5-dihydro-1H-pyrrol-3-yl)-acetic acid eth…
CCDC 749971: Experimental Crystal Structure Determination
2010
Related Article: Y.Rousselin, N.Sok, F.Boschetti, R.Guilard, F.Denat|2010|Eur.J.Org.Chem.|2010|1688|doi:10.1002/ejoc.200901183
La adopción abierta en el derecho español
2016
El reconocimiento del derecho del menor adoptado a mantener contactos o relaciones con sus familiares de origen y sus anteriores acogedores representa un nuevo modelo de adopción en el Derecho español, denominado “adopción abierta”, recientemente introducida por la Ley 26/2015, de 28 de julio, que reforma el sistema español de protección de la infancia y la adolescencia. La nueva regulación se inspira en el Derecho de otros países pero tiene precedentes en ciertas normas autonómicas españolas The recognition of the right of the adopted children to maintain contacts or relations with their families of origin and previous welcoming, represents a new model of adoption in the Spanish law, which…
Portales educativos : la producción de materiales didácticos digitales
2018
El objetivo principal de este artículo es conocer cómo se organiza la producción de los materiales didácticos digitales de creación propia de portales institucionales de recursos educativos, tomando como referencia la perspectiva de sus principales responsables. En concreto, se analiza el caso del repositorio institucional de recursos de la Comunidad Autónoma de Canarias, Ecoescuela 2.0; y del portal de recursos educativos en abierto Procomún (Ministerio de Educación, Cultura y Deporte). Con este estudio se pretende dar respuesta a distintas cuestiones relacionadas con la autoría de los materiales, el proceso y desarrollo, así como los criterios que establecen los portales institucionales p…
Performance optimisation of biological pathway data storage, retrieval, analysis and its interactive visualisation
2018
The aim of this research was to optimise the performance of the storage, retrieval, analysis and interactive visualisation of biomolecular pathways data. This was achieved by the adoption of new technologies and a variety of highly optimised data structures, algorithms and strategies across the different layers of the software. The first challenge to overcome was the creation of a long-lasting, large-scale web application to enable pathways navigation; the Pathway Browser. This tool had to aggregate different modules to allow users to browse pathway content and use their own data to perform pathway analysis. Another challenge was the development of a high-performance pathway analysis tool t…