Search results for "Opus"
showing 10 items of 346 documents
Identification and functional expression of HCx31.9, a novel gap junction gene
2002
By combining in silico and bench molecular biology methods we have identified a novel human gap junction gene that encodes a protein designated HCx31.9. We have determined its human chromosomal location and gene structure, and we have identified a putative mouse ortholog, mCx30.2. We have observed the presence of HCx31.9 in human cerebral cortex, liver, heart, spleen, lung, and kidney and the presence of mCx30.2 in mouse cerebral cortex, liver and lung. Moreover, preliminary data on the electrophysiological properties of HCx31.9 have been obtained by functional expression in paired Xenopus oocytes and in transfected N2A cells.
Activation by Acidic pH of CLC-7 Expressed in Oocytes from Xenopus laevis
2002
ClC chloride channels are important in diverse physiological functions such as transepithelial transport, cell volume regulation, excitability, and acidification of intracellular organelles. We have investigated the expression of CLC-7 in oocytes from Xenopus laevis with the two electrode voltage clamp technique and Western blot analysis. Using a specific antibody against CLC-7, we found an approximately 80 kDa protein in oocytes, previously injected with CLC-7-cRNA. In voltage clamp experiments on ClC-7-cRNA-injected oocytes, no current changes were detected at normal pH (7.4). However, acidification of the Ringer solution to pH values between 6 and 4 revealed strong currents which reverse…
Intracellular accumulation of l-Arg, kinetics of transport, and potassium leak conductance in oocytes from Xenopus laevis expressing hCAT-1, hCAT-2A,…
2004
AbstractCationic amino acid transporters play an important role in the intracellular supply of l-Arg and the generation of nitric oxide. Since the transport of l-Arg is voltage-dependent, we aimed at determining the intracellular l-Arg concentration and describing the transport of l-Arg in terms of Michaelis–Menten kinetics, taking into account membrane voltage. The human isoforms of the cationic amino acid transporters, hCAT-1, hCAT-2A, and hCAT-2B, were expressed in oocytes from Xenopus laevis and studied with the voltage clamp technique and in tracer experiments. We found that l-Arg was concentrated intracellularly by all hCAT isoforms and that influx and efflux, in the steady state of e…
Ecos de la Junta democrática y de la oposición en el diario ABC (1974-75): la ruptura como pesadilla
2012
International audience; La creación de la Junta democrática como plataforma antifranquista se proponía romper con las instituciones del Régimen iniciando una lucha pacífica que debía permitir una democratización radical del estado. La ruptura con las instituciones del franquismo debía ser llevada a cabo gracias a un gobierno provisional representativo de todas las fuerzas de oposición. Las numerosas acciones y comunicaciones a los órganos de prensa internacionales de la Comisión Exterior de la Junta democrática de España, dirigida por José Vidal Beneyto, permitieron a la JDE darse a conocer a los países europeos siendo aún ilegal dentro del país. Los artículos del diario ABC de 1974-75 se h…
The main determinant of furosemide inhibition on GABA(A) receptors is located close to the first transmembrane domain.
1998
Inhibitory GABA(A) receptors are regulated by numerous allosteric modulators, the most receptor-subtype specific of which is furosemide. It recognises receptors of the subunit composition alpha6beta2/3gamma2, restricted to cerebellar granule cells. To locate furosemide's site of action we constructed chimeras of the furosemide-sensitive alpha6 and the furosemide-insensitive alpha1 subunit, and expressed and studied them together with the beta3 and gamma2 subunits in Xenopus oocytes by the two-electrode voltage clamp technique. The inhibition of GABA-induced currents by furosemide mainly depended on a short domain proximal to the first transmembrane region of the alpha6 subunit.
Acoustic differences between populations of western and eastern Bonelli's Warblers (Phylloscopus bonelli, Sylviidae)
1982
Sonographic analyses of songs and calls of Bonelli's Warbler (Phylloscopus bonelli) show important structural differences between the geographically isolated western (P.b. bonelli) and eastern (P.b. orientalis) populations. Playback experiments in the field and some morphological differences suggest that these populations may be separating into 2 species.
Rhythmische Änderungen der Reaktion auf verschiedene olfaktorische Reize beim Krallenfrosch (Xenopus laevis)
1975
The behavioural response ofXenopus laevis to olfactory cues shows different rhythmical oscillations which do not depend on the chemical nature and the concentration of the stimulus itself. Habituation to such cues occurs very slowly, pointing to their special importance in the feeding behaviour of this species.
Evaluation of the effectiveness of drug prevention programs: Analysis of the international scientific production (2002–2011)
2015
s / Drug and Alcohol Dependence 146 (2015) e202–e284 e205 Oxytocin effects on human aggressive responding Joseph L. Alcorn, Nadeeka Dias, N. Rathnayaka, Joy Schmitz, C. Green, Scott D. Lane Department of Psychiatry & Behavioral Sciences, School of Medicine, Program in Neuroscience, Graduate School of Biomedical Sciences, University of Texas Health Science Center – Houston, Houston, TX, United States Aims: In the search for interventions aimed at improving the social functioning of individuals with substance use disorders (SUD), the neuropeptide oxytocin (OT) and the oxytonergic system may hold promise as an intervention strategy for promoting prosocial behaviors. Acute administration of OT …
The cAMP Pathway Sensitizes VR1 Expressed in Oocytes from <i>Xenopus laevis</i> and in CHO Cells
2003
The vanilloid receptor 1 (VR1) is a heat-activated cation channel which also responds to capsaicin and other chemical stimuli. Protein kinase C has a stimulatory effect on VR1 activity, either alone or after activation with capsaicin. The influence of the cAMP-signaling pathway on the effects of capsaicin is controversial. To clarify this, the actions of capsaicin and the modulatory effects of forskolin, pCPT-cAMP, and isobutylmethylxanthine were studied in <i>Xenopus laevis</i> oocytes expressing rat VR1 and in CHO cells expressing human VR1. Capsaicin activated the VR1 channel and increased the intracellular calcium concentration. The effects of capsaicin were enhanced by fors…
Identification of Novel Molecular Components of the Photoreceptor Connecting Cilium by Immunoscreens
2002
Abstract The connecting cilium of photoreceptor cells is the only intracellular link between the morphologically, functionally and biochemically different compartments of the inner and outer segments. The non-motile modified cilium plays an important role in the organization and the function of photoreceptor cells, namely in delivery and turnover of enzymes and substrates of the visual transduction cascade, and the photosensitive membranes of the outer segment. The protein components of the cilium participate in the intracellular transport through the cilium, in the outer segment disk morphogenesis and in the maintenance of discrete membrane domains. In order to identify yet unknown cytoske…