Search results for "PCR"
showing 10 items of 438 documents
Mixed infection of Pectobacterium carotovorum subsp. Carotovorum and P. carotovorum subsp. Brasiliensis in tomato stem rot in Italy
2016
Grafted tomato plants grown in a soilless culture system in heated greenhouses in Sicily were affected by stem rot disease. Symptoms consisted of dark brown longitudinal discoloration of the basal part of the stem and petioles. Longitudinal sections of the stem revealed brown watersoaked or soft-rotted pith tissue and internal vascular discoloration. Bacterial isolates with two different colony morphologies were obtained from symptomatic tissues. Isolates were identified as Pectobacterium spp. on the basis of biochemical and molecular analyses. The analysis of the 16S rRNA gene sequence and of the concatenated sequences of the housekeeping genes rpoS and pgi revealed that the isolates of th…
Prokaryoottien PCR-perustaisten havaintomenetelmien kehittäminen ja eliöiden tunnistaminen syvän kaivoksen kivi- ja vesinäytteistä
2008
Flavobacterium columnare in Finnish fish farming : characterisation and putative disease management strategies
2005
Lotta-Riina Suomalainen selvitti väitöskirjatyössään syitä flavobakteeritaudin lisääntymiselle. Flavobacterium columnare -bakteerin aiheuttama kalakuolleisuus on vakava uhka suomalaiselle poikaskalan tuotannolle. Flavobakteeri-infektiot muodostavat suurimman osan kalanviljelyssä diagnostisoiduista tautitapauksista ja niiden haitta elinkeinolle on merkittävä. Flavobacterium columnare -bakteeri aiheuttaa kalanpoikasille vakavia kidus- ja ihotulehduksia. Tappiot kalanviljelijälle ovat mittavat, sillä jopa 100 % sairastuneista kaloista voi kuolla. Tauti esiintyy kesäaikaan yleensä viljellyillä lohikaloilla. Sen esiintyminen on lisääntynyt viime vuosien aikana, mutta syitä siihen ei tiedetä. The…
CHARACTERIZATION OF A STRAIN OF PEPINO MOSAIC VIRUS FOUND IN SICILY
2006
During winter 2005, an unusual virus-like yellow leaf disorder associated to interveinal leaf yellowing and marbling on fruits, was observed in some greenhouse tomato crops in Sicily, exactly in province of Ragusa. Two leaves from 250 symptomatic tomato plants were serologically tested by DAS-ELISA technique using the sera to Tomato spotted wilt virus (TSWV), Impatiens necrotic spot virus (INSV), Tobacco mosaic virus (TMV), Cucumber mosaic virus (CMV) and Pepino mosaic virus (PepMV). Of the 250 samples tested, 215 were positive to PepMV presence. This virus was mechanically transmitted to Cucumis sativus “Cubit”, Datura metel, Nicotiana benthamiana and tomato “Rio Grande”. The host range of…
STUDIO DELLA BIODISTRIBUZIONE DI SOSTANZE BIOLOGICAMENTE ATTIVE IN ORGANISMI ACQUATICI
2022
An international perspective on hospitalized patients with viral community-acquired pneumonia
2019
Background Who should be tested for viruses in patients with community acquired pneumonia (CAP), prevalence and risk factors for viral CAP are still debated. We evaluated the frequency of viral testing, virus prevalence, risk factors and treatment coverage with oseltamivir in patients admitted for CAP. Methods Secondary analysis of GLIMP, an international, multicenter, point-prevalence study of hospitalized adults with CAP. Testing frequency, prevalence of viral CAP and treatment with oseltamivir were assessed among patients who underwent a viral swab. Univariate and multivariate analysis was used to evaluate risk factors. Results 553 (14.9%) patients with CAP underwent nasal swab. Viral CA…
Seasonal Human Coronavirus Respiratory Tract Infection in Recipients of Allogeneic Hematopoietic Stem Cell Transplantation
2021
Abstract Background Little is known about characteristics of seasonal human coronaviruses (HCoVs) (NL63, 229E, OC43, and HKU1) after allogeneic stem cell transplantation (allo-HSCT). Methods This was a collaborative Spanish and European bone marrow transplantation retrospective multicenter study, which included allo-HSCT recipients (adults and children) with upper respiratory tract disease (URTD) and/or lower respiratory tract disease (LRTD) caused by seasonal HCoV diagnosed through multiplex polymerase chain reaction assays from January 2012 to January 2019. Results We included 402 allo-HSCT recipients who developed 449 HCoV URTD/LRTD episodes. Median age of recipients was 46 years (range,…
VALORIZZAZIONE DEL GERMOPLASMA AUTOCTONO SICILIANO DI POMODORO
2014
Environmental DNA analysis indicates that migration barriers are decreasing the occurrence of European eel (Anguilla anguilla) in distance from the s…
2020
Abstract The European eel (Anguilla L.) is considered critically endangered by the IUCN Red List, and recruitment remains low. One of the challenges for the species today is migration barriers that limit their habitat. Along the Norwegian coast, like in other countries, the abundance of eels appears to decrease with distance from the sea. This pattern may be a result of factors like water temperature, water quality, competition, and habitat suitability and availability. This study aims to use environmental DNA (eDNA) analysis to investigate the potential relationship between migration barriers and the decreasing occurrence of eels in distance from the sea by the coast of southern Norway. Si…
Estimating trematode prevalence in snail hosts using a single-step duplex PCR: how badly does cercarial shedding underestimate infection rates?
2014
Background: Trematode communities often consist of different species exploiting the same host population, with two or more trematodes sometimes co-occuring in the same host. A commonly used diagnostic method to detect larval trematode infections in snails has been based on cercarial shedding, though it is often criticized as inaccurate. In the present study we compare infection prevalences determined by cercarial emission with those determined, for the first time, by molecular methods, allowing us to quantify the underestimation of single and double infections based on cercarial emission. We thus developed a duplex PCR for two host-parasite systems, to specifically differentiate between sin…