Search results for "PLASMA"

showing 10 items of 4043 documents

Cannabidiol prevents priming- and stress-induced reinstatement of the conditioned place preference induced by cocaine in mice

2021

[Background]: Cocaine dependence is an important problem without any effective pharmacological treatment. Some preclinical studies have suggested that cannabidiol (CBD), a component of the Cannabis sativa plant, could be useful for the treatment of cocaine use disorders.

MaleConditioning ClassicalPharmacologyCocaine dependenceSocial DefeatSocial defeatCocaine-Related DisordersMice03 medical and health sciences0302 clinical medicineCannabinoid Receptor ModulatorsmedicineAnimalsCannabidiolPharmacology (medical)Dopamine transporterPharmacologyDopamine Plasma Membrane Transport ProteinsBehavior Animalbiologybusiness.industryVentral Tegmental Areafood and beveragesmedicine.diseasebiology.organism_classificationConditioned place preference030227 psychiatryVentral tegmental areaDisease Models AnimalPsychiatry and Mental healthmedicine.anatomical_structurebiology.proteinCannabisbusinessPriming (psychology)Cannabidiol030217 neurology & neurosurgerymedicine.drugJournal of Psychopharmacology
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[18F]PR04.MZ PET/CT Imaging for Evaluation of Nigrostriatal Neuron Integrity in Patients With Parkinson Disease.

2020

Introduction Degeneration of dopaminergic, nigrostriatal neurons is the hallmark of Parkinson disease (PD), and PET quantification of dopamine transporters is a widely accepted method for differential diagnosis between idiopathic PD and essential tremor. [18F]PR04.MZ is a new PET tracer with excellent imaging properties allowing for precise quantification of striatal and extrastriatal dopamine transporter. Here we describe our initial experience with [18F]PR04.MZ PET/CT in a larger cohort of healthy controls and PD patients as a proof-of-concept study for this tracer. Methods Eighteen healthy subjects, 19 early PD patients (Hoehn-Yahr I–II), and 13 moderate-advanced PD patients (Hoehn-Yahr …

MaleDopamineCaudate nucleusPET imagingSubstantia nigra[18F]PR04.MZ030218 nuclear medicine & medical imagingCohort Studies03 medical and health sciences0302 clinical medicineDopaminePositron Emission Tomography Computed TomographyMedicineHumansRadiology Nuclear Medicine and imagingdopamine transporterDopamine transporterAgedNeuronsDopamine Plasma Membrane Transport ProteinsbiologyEssential tremorbusiness.industryPars compactaPutamenDopaminergicParkinson DiseaseGeneral MedicineOriginal ArticlesMiddle Agedmedicine.diseaseSubstantia Nigranervous system030220 oncology & carcinogenesisbiology.proteinmovement disordersFemalebusinessNuclear medicinemedicine.drugClinical nuclear medicine
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Evaluation of remnant cholesterol levels and Monocyte-to-HDL-cholesterol ratio in South Asian patients with acute coronary syndrome.

2021

Abstract Background and aims In the present study, we aimed to compare the clinical and coronary angiography features between South Asian and Caucasian patients with Acute Coronary Syndrome (ACS). In particular, we focused our analysis on the evaluation of recent cardiovascular risk markers, such as remnant cholesterol, corresponding to all plasma cholesterol minus HDL-C (high-density lipoprotein cholesterol) and LDL-C (low-density lipoprotein cholesterol), and the Monocyte-to-HDL-cholesterol ratio. We also compared values of several lipoprotein ratios and the Platelet-to-lymphocyte ratio, accurate predictors of coronary events and coronary artery disease. Methods and results We recruited 4…

MaleEndocrinology Diabetes and MetabolismMedicine (miscellaneous)Coronary Artery Disease030204 cardiovascular system & hematologyCoronary AngiographyMonocyte-to-HDL-cholesterol ratioMonocytesCoronary artery diseasechemistry.chemical_compoundLeukocyte Count0302 clinical medicinePrevalenceNutrition and DieteticsRemnant cholesterolMedical recordMiddle AgedCholesterolItalyHDL/cholesterol ratiolipids (amino acids peptides and proteins)FemalePlatelet-to-lymphocyte ratio.Cardiology and Cardiovascular MedicineAdultmedicine.medical_specialtyAcute coronary syndromeSouth asia030209 endocrinology & metabolismRisk AssessmentWhite People03 medical and health sciencesAsian PeoplePredictive Value of TestsInternal medicineStatistical significancemedicineHumansAcute Coronary SyndromeRetrospective StudiesPlasma LipidAsianCholesterolbusiness.industryCholesterol HDLmedicine.diseasechemistryHeart Disease Risk FactorsbusinessBiomarkersLipoproteinNutrition, metabolism, and cardiovascular diseases : NMCD
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Epidemiology of Toxoplasma and CMV serology and of GBS colonization in pregnancy and neonatal outcome in a Sicilian population

2013

Background: Aim of our study is to analyze the immunological status in pregnancy for two main TORCH agents, Toxoplasma and Cytomegalovirus (CMV), and the results of group B streptococcus (GBS) screening, assessing the risk for congenital infection in a population from Palermo, Italy. Methods: We retrospectively analyzed the medical records of all inborn live newborns who were born in our division during 2012, gathering information about the mother, the pregnancy and neonatal hospitalization at birth. Whenever data were available, we categorized the serologic status of the mothers for Toxoplasma and CMV. We also considered the results of rectal and vaginal swabs for GBS. We compared the resu…

MaleEpidemiologyAntibodies ProtozoanCytomegalovirusmedicine.disease_causeAntibodies ViralGroup BInfant Newborn DiseasesSerologySettore MED/38 - Pediatria Generale E SpecialisticaPregnancyRisk FactorsPrenatal DiagnosisEpidemiologyPrevalencePregnancy Complications InfectiousIntrauterine infectionSicilyeducation.field_of_studyPregnancy OutcomeCMVMiddle AgedAntibodies BacterialAntibodies Anti-IdiotypicCytomegalovirus InfectionsFemaleToxoplasmaToxoplasmosisAdultmedicine.medical_specialtyAdolescentPopulationCongenital cytomegalovirus infectionGBSRisk AssessmentStreptococcus agalactiaeYoung AdultStreptococcal InfectionsmedicineAnimalsHumanseducationRetrospective StudiesPregnancybusiness.industryResearchInfant Newbornmedicine.diseaseToxoplasmosisStreptococcus agalactiaeImmunoglobulin GImmunologybusinessItalian Journal of Pediatrics
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Ex vivo and in vivo evaluation of [18F]PR04.MZ in rodents: a selective dopamine transporter imaging agent.

2009

N-4-Fluorobut-2-yn-1-yl-2beta-carbomethoxy-3beta-phenyltropane (PR04.MZ) has been developed as dopamine transporter (DAT) ligand for molecular imaging. It contains a terminally fluorinated, conformationally constrained nitrogen substituent that is well suited for the introduction of fluorine-18. The present report describes the pharmacological characterisation of [18F]PR04.MZ. The ligand shows an IC50 value of 2 nM against human DAT, whereas the IC50 value against human serotonin transporter and human noradrenalin transporter are lower (110 nM and 22 nM, respectively). Furthermore, its ex vivo organ distribution, its binding profile in the rat brain and reversibility of binding were examine…

MaleFluorine RadioisotopesDopamine Plasma Membrane Transport ProteinsBiochemistryCell LineRats Sprague-DawleyIn vivoDrug DiscoveryAnimalsHumansTissue DistributionGeneral Pharmacology Toxicology and PharmaceuticsSerotonin transporterDopamine transporterPharmacologySerotonin Plasma Membrane Transport ProteinsDopamine Plasma Membrane Transport ProteinsNorepinephrine Plasma Membrane Transport ProteinsbiologyChemistryOrganic ChemistryTransporterLigand (biochemistry)Imaging agentRatsBiochemistryPositron-Emission Tomographybiology.proteinBiophysicsMolecular MedicineRadiopharmaceuticalsEx vivoTropanesChemMedChem
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Quantitation of GABA transporter 3 (GAT3) mRNA in rat brain by competitive RT-PCR.

1999

Gamma-amino butyric acid is the major inhibitory neurotransmitter in the brain. GABA transporters (GATs) remove GABA from the synaptic cleft. Till now, five distinct GABA transporters have been cloned and termed consecutively GAT1 to GAT4 and vGAT. To study the mechanisms by which tolerance and dependence associated with drugs enhancing GABAergic transmission is brought upon we analysed the mRNA expression levels of GATs in various brain regions under different conditions. In this paper, we describe our protocol for measurement of GAT3 mRNA expression, and its validation through control experiments for the various steps. We performed competitive reverse transcription and polymerase chain re…

MaleGABA Plasma Membrane Transport ProteinsDNA ComplementarySynaptic cleftBiologyBinding CompetitiveRibonucleasesAnimalsRNA MessengerReceptorgamma-Aminobutyric AcidGel electrophoresisBrain ChemistryMessenger RNAReverse Transcriptase Polymerase Chain ReactionGeneral NeuroscienceWild typeMembrane Transport ProteinsReproducibility of ResultsTransporterRats Inbred StrainsMolecular biologyReverse transcriptaseRatsReal-time polymerase chain reactionBiochemistryCarrier ProteinsBrain research. Brain research protocols
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Tiagabine, a gamma-amino-butyric acid transporter inhibitor impairs spatial learning of rats in the Morris water-maze.

2002

Abstract γ-Amino-butyric acid (GABA) is cleaved from the synaptic cleft by uptake via specific transporters. Inhibition of such transporters increases the effectiveness of physiologically released GABA. Increased GABAergic neurotransmission has an impact on learning and memory. Therefore, effects of tiagabine, a GABA-transporter inhibitor, were investigated on spatial orientation in the Morris water-maze. Rats were given four training trials per day for 4 days and a probe trial without platform on the 5th day. Compared to saline treated rats, rats treated daily with 20 mg/kg tiagabine showed impaired learning during the acquisition trials. Retrieval was impaired in rats treated only at the …

MaleGABA Plasma Membrane Transport ProteinsSynaptic cleftTiagabinemedicine.medical_treatmentNipecotic AcidsMorris water navigation taskOrganic Anion TransportersPharmacologyBehavioral Neurosciencechemistry.chemical_compoundMemorymedicineGABA transporterAnimalsNeurotransmitterMaze LearningTiagabineSalineGABA AgonistsSwimmingbiologyMembrane ProteinsMembrane Transport ProteinsTransporterRats Inbred StrainsReceptors GABA-ARatschemistrybiology.proteinReuptake inhibitorCarrier ProteinsNeurosciencemedicine.drugBehavioural brain research
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Gelatinases and their tissue inhibitors in a group of subjects with metabolic syndrome.

2013

Aim To evaluate matrix metalloproteases (MMP)-2 and MMP-9 and tissue inhibitor of metalloproteases (TIMP)-1 and TIMP-2 in a group of subjects with metabolic syndrome (MS) subdivided according to the presence or absence of diabetes mellitus. Methods We examined in 90 subjects (51 men and 39 women) with MS, defined following the International Diabetes Federation criteria, and subsequently subdivided into diabetic subjects (22 men and 11 women) and nondiabetic subjects s (29 men and 28 women), the plasma concentrations of MMP-2 and MMP-9 and of TIMP-1 and TIMP-2 using specific enzyme-linked immunosorbent assay kits. Results We found a significant increase in plasma concentrations of MMP-2, MMP…

MaleGelatinasesmedicine.medical_specialtyMatrix metalloproteinase insulin resistanceMatrix metalloproteinaseBiologyMatrix Metalloproteinase InhibitorsGeneral Biochemistry Genetics and Molecular BiologyDiabetes mellitusInternal medicinemedicineMatrix metalloproteasesHumansInternational diabetes federationMetabolic SyndromeMetalloproteinaseTissue Inhibitor of Metalloproteinase-2Tissue Inhibitor of Metalloproteinase-1General MedicineMiddle Agedmedicine.diseaseEndocrinologyMatrix Metalloproteinase 9GelatinasesPlasma concentrationMatrix Metalloproteinase 2FemaleMetabolic syndromeBiomarkersJournal of investigative medicine : the official publication of the American Federation for Clinical Research
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Soft Tissue Response to Titanium Abutments with Different Surface Treatment: Preliminary Histologic Report of a Randomized Controlled Trial

2016

The aim of this preliminary prospective RCT was to histologically evaluate peri-implant soft tissues around titanium abutments treated using different cleaning methods. Sixteen patients were randomized into three groups: laboratory customized abutments underwent Plasma of Argon treatment (Plasma Group), laboratory customized abutments underwent cleaning by steam (Steam Group), and abutments were used as they came from industry (Control Group). Seven days after the second surgery, soft tissues around abutments were harvested. Samples were histologically analyzed. Soft tissues surrounding Plasma Group abutments predominantly showed diffuse chronic infiltrate, almost no acute infiltrate, with …

MaleGenetics and Molecular Biology (all)Pathologymedicine.medical_specialtyPlasma GasesArticle SubjectCleaning methodsImmunology and Microbiology (all)Statistical differencelcsh:MedicineDental AbutmentsBiochemistryGeneral Biochemistry Genetics and Molecular BiologySoft Tissue Responselaw.inventionPlasma Gase03 medical and health sciences0302 clinical medicineRandomized controlled trialDental AbutmentslawCollagen; Female; Humans; Male; Middle Aged; Mouth; Plasma Gases; Titanium; Wound Healing; Dental AbutmentsHumansMedicineddc:610TitaniumMouthWound HealingDental AbutmentBiochemistry Genetics and Molecular Biology (all)General Immunology and Microbiologybusiness.industrySignificant differencelcsh:RSoft tissue030206 dentistryGeneral MedicineMiddle AgedClinical StudyFemaleCollagenWound healingbusinessHuman030215 immunologyBiochemistry; Genetics and Molecular Biology (all); Immunology and Microbiology (all)BioMed Research International
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Conservative Surgical Treatment of Bisphosphonate-Related Osteonecrosis of the Jaw with Er,Cr:YSGG Laser and Platelet-Rich Plasma: A Longitudinal Stu…

2018

Introduction. The management of bisphosphonate-related osteonecrosis of the jaw (BRONJ), with no evidence-based guidelines, remains controversial. We aimed to evaluate the efficiency of a conservative surgical treatment combining Er,Cr:YSGG laser and platelet-rich plasma (PRP) for the treatment of BRONJ in cancer patients. Methods. We performed a longitudinal cohort study. Inclusion criteria were (1) age ≥ 18 years; (2) cancer diagnosis; (3) treatment with NBP because of the underlying cancer. Results. We consecutively recruited ten patients diagnosed with BRONJ in stage I or II. These patients underwent a surgical laser-assisted therapy together with autologous PRP. At the latest follow-up…

MaleGenetics and Molecular Biology (all)medicine.medical_specialtyLongitudinal studyArticle SubjectImmunology and Microbiology (all)medicine.medical_treatmentSettore MED/19 - Chirurgia Plasticalcsh:MedicineLasers Solid-StateSettore MED/08 - Anatomia PatologicaBiochemistryGeneral Biochemistry Genetics and Molecular BiologySettore MED/01 - Statistica Medica03 medical and health sciences0302 clinical medicineSettore MED/28 - Malattie OdontostomatologichemedicineHumansLongitudinal StudiesProspective StudiesSurgical treatmentProspective cohort studyAgedAged 80 and overSurgical approachBiochemistry Genetics and Molecular Biology (all)Bone Density Conservation AgentsDiphosphonatesGeneral Immunology and MicrobiologyPlatelet-Rich Plasmabusiness.industrylcsh:RCancer030206 dentistryGeneral MedicineBisphosphonatemedicine.diseaseSurgery030220 oncology & carcinogenesisPlatelet-rich plasmaClinical StudyBisphosphonate-Associated Osteonecrosis of the JawFemaleOsteonecrosis of the jawbusinessBiochemistry Genetics and Molecular Biology (all); Immunology and Microbiology (all)
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