Search results for "PPRE"

showing 10 items of 2084 documents

Mosaic Qβ coats as a new presentation model

1998

The new protein carrier was developed on the basis of recombinant RNA phage Qbeta capsid. C-terminal UGA extension of the short form of Qbeta coat, so-called A1 extension, served as a target for presentation of foreign peptides on the outer surface of mosaic Qbeta particles. In conditions of enhanced UGA suppression, the proportion of A1-extended to short coats in mosaic particles dropped from 48% to 14%, with an increase of the length of A1 extension. A model insertion, short preS1 epitope 31-DPAFR-35 of hepatitis B surface antigen, demonstrated superficial location on the mosaic Qbeta particles and ensured specific antigenicity and immunogenicity.

AntigenicityRecombinant Fusion ProteinsGenetic VectorsBiophysicsBiologyHepatitis b surface antigenBiochemistryEpitopelaw.inventionCapsid assemblyMiceCapsidPhage QβPeptide LibraryStructural BiologylawGeneticsAnimalsHepatitis B virus preS1Cloning MolecularMolecular BiologyAllolevivirusMice Inbred BALB CCoat protein UGA suppressionVirus AssemblyImmunogenicityA1 extensionRNACell BiologyImmunogenicityVirologyMolecular biologyCapsidCarrier proteinCodon TerminatorRecombinant DNACapsid ProteinsFEBS Letters
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5-Fluorouracil Selectively Kills Tumor-Associated Myeloid-Derived Suppressor Cells Resulting in Enhanced T Cell–Dependent Antitumor Immunity

2010

AbstractMyeloid-derived suppressor cells (MDSC) accumulate in the spleen and tumor bed during tumor growth. They contribute to the immune tolerance of cancer notably by inhibiting the function of CD8(+) T cells. Thus, their elimination may hamper tumor growth by enhancing antitumor T-cell functions. We have previously reported that some anticancer agents relied on T cell–dependent anticancer responses to achieve maximal efficacy. However, the effect of anticancer agents on MDSC has remained largely unexplored. In this study, we observed that gemcitabine and 5-fluorouracil (5FU) were selectively cytotoxic on MDSC. In vivo, the treatment of tumor-bearing mice with 5FU led to a major decrease …

Antimetabolites AntineoplasticCancer ResearchT-LymphocytesT cellMice NudeApoptosisCD8-Positive T-LymphocytesBiologyDeoxycytidineImmune toleranceMiceImmune systemCell Line TumormedicineAnimalsCytotoxic T cellMice Inbred BALB CDendritic CellsT lymphocyteFlow CytometryGemcitabineMice Inbred C57BLmedicine.anatomical_structureOncologyCell cultureImmune SystemImmunologyMyeloid-derived Suppressor CellCancer researchFluorouracilNeoplasm TransplantationCD8Cancer Research
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The interplay between the immune system and chemotherapy: emerging methods for optimizing therapy.

2013

Preclinical studies have revealed an unexpected ability of the immune system to contribute to the success of chemotherapy and radiotherapy. Anticancer therapies can trigger immune system activation by promoting the release of danger signals from dying tumor cells and/or the elimination of immunosuppressive cells. We have, however, recently discovered that some chemotherapies, such as 5-fluorouracil and gemcitabine, exert conflicting effects on anticancer immune responses. Although 5-fluorouracil and Gem selectively eliminated myeloid-derived suppressive cells in tumor-bearing rodents, these chemotherapies promoted the release of IL-1β and the development of pro-angiogenic IL-17-producing CD…

Antimetabolites Antineoplasticmedicine.medical_treatmentImmunologyInterleukin-1betaDeoxycytidineImmune systemImmunityNeoplasmsmedicineImmunology and AllergyAnimalsHumansImmunologic FactorsChemotherapyImmunity Cellularbusiness.industryImmunosuppressionInflammasomeChemoradiotherapyGemcitabineGemcitabineRadiation therapyImmunologyMyeloid-derived Suppressor CellCancer researchTh17 CellsFluorouracilbusinessmedicine.drugExpert review of clinical immunology
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Resveratrol modulates the levels of microRNAs targeting genes encoding tumor-suppressors and effectors of TGFbeta signaling pathway in SW480 cells.

2010

International audience; Resveratrol (trans-3,4',5-trihydroxystilbene) is a natural antioxidant with cardiovascular and cancer preventive properties that is currently at the stage of pre-clinical studies for human cancer prevention. Beside its known effects on protein coding genes, one possible mechanism for resveratrol protective activities is by modulating the levels of non-coding RNAs. Here, we analyzed the effects of resveratrol on microRNA populations in human SW480 colon cancer cells. We establish that resveratrol treatment decreases the levels of several oncogenic microRNAs targeting genes encoding Dicer1, a cytoplasmic RNase III producing mature microRNAs from their immediate precurs…

Antineoplastic AgentsSmad ProteinsResveratrolBiochemistryAntioxidantsArticleTransforming Growth Factor beta1chemistry.chemical_compoundTGFβTransforming Growth Factor betaCell Line TumormicroRNAStilbenesPTENHumansRibonuclease III[ SDV.BBM ] Life Sciences [q-bio]/Biochemistry Molecular BiologyPharmacologyOncogene ProteinsbiologyEffectorTumor Suppressor ProteinsTransforming growth factor betaMolecular biologyColon cancer; microRNAs; miR-663; Resveratrol; SW480 cells; TGFβmiR-663Cell biologyColon cancerMicroRNAsSW480 cellschemistryResveratrolbiology.proteinSignal transductionTransforming growth factorSignal Transduction
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"Il viaggio" di Tomasi di Lampedusa. Uno sguardo antropologico sullo spazio dell'attraversamento

2011

Antropologia Semiotica Viaggio Rappresentazione Letteratura
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Controlling protein interactions in blood for effective liver immunosuppressive therapy by silica nanocapsules

2020

Immunosuppression with glucocorticoids is a common treatment for autoimmune liver diseases and after liver transplant, which is however associated with severe side-effects. Targeted delivery of glucocorticoids to inflammatory cells, e.g. liver macrophages and Kupffer cells, is a promising approach for minimizing side effects. Herein, we prepare core–shell silica nanocapsules (SiO2 NCs) via a sol–gel process confined in nanodroplets for targeted delivery of dexamethasone (DXM) for liver immunosuppressive therapy. DXM with concentrations up to 100 mg mL−1 in olive oil are encapsulated while encapsulation efficiency remains over 95% after 15 days. Internalization of NCs by non-parenchymal muri…

Apolipoprotein BCell SurvivalLiver cytologyPharmacologybehavioral disciplines and activitiesDexamethasoneNanocapsulesProinflammatory cytokine//purl.org/becyt/ford/1 [https]MiceDrug Delivery SystemsDrug StabilityNanocapsulesQuímica Coloidalmental disordersBlood plasma//purl.org/becyt/ford/1.4 [https]AnimalsHumansIMMUNOSUPPRESSIVE THERAPYTissue DistributionGeneral Materials ScienceColloidsImmunosuppression TherapybiologyClusterinChemistryCiencias QuímicasSILICA NANOCAPSULESSilicon DioxideBlood proteinsPROTEIN INTERACTIONSDEXAMETHASONELiverbiology.proteinPEGylationCytokinesCIENCIAS NATURALES Y EXACTASImmunosuppressive AgentsHeLa CellsNanoscale
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El aprendizaje a distancia y los adolescentes en el momento de la pandemia: espacialidad e interacciones

2021

Il capitolo esplora alcune modalità attraverso cui gli studenti adolescenti hanno vissuto e stanno vivendo lunghi periodi di apprendimento a distanza a causa delle misure anti-pandemia. Lo studio qui proposto si basa su una ricerca che ho condotto nel 2021 con un approccio geografico e semiotico in una scuola secondaria di Palermo. I destinatari sono sette studenti dello stesso anno scolastico, di età compresa tra i 16 e i 17 anni, a cui è stato chiesto di elaborare un breve testo che descrive come avevano affrontato in tutto o in parte i periodi di formazione a distanza. El objetivo de este trabajo es explorar algunas de las formas en que los estudiantes adolescentes han vivido y están exp…

Apprendimento a distanza Adolescenti Scuola Geografia culturale SpazioAprendizaje a distancia Adolescentes Escuela Geografia cultural Espacio
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Valori pedagogici e criticità dell'elearning

2014

Cos’è un modello sostenibile? Come si connota, in particolare, un modello di formazione sostenibile? E, all’interno di questo, come si declina un modello di formazione ispirato all’elearning? Quali sono le ragioni pedagogiche ed economiche che hanno richiesto/richiedono l’utilizzo di questo approccio metodologico? Scopo di questo breve lavoro è di rispondere a questi interrogativi, privilegiando, in particolare, da una prospettiva pedagogica, la riflessione sull’elearning, oggetto, questa, spesso, di punti di vista radicalmente diversi che si situano, radicalizzando il discorso, tra una posizione fortemente contestativa della formazione online accusata di operare, spesso, forme di netta sep…

Apprendimento digitale Elearning EducazioneSettore M-PED/01 - Pedagogia Generale E Sociale
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Mündliche Kompetenz und Bewusstsein beim unterrichtlichen Fremdsprachenlernen

2014

Das Buch befasst sich mit dem Zusammenspiel von kognitiven, emotionalen und motivationalen Faktoren beim Aufbau von mündlicher Kompetenz in Deutsch als Tertiärsprache bei erwachsenen Italienern. Es stützt sich dabei auf ein interdisziplinäres Vorgehen, das theoretisch erörtert und in seiner Methodenwahl ausführlich dargelegt wird, insbesondere die Videografie sowie intro-/retrospektive Verfahren. Die Ergebnisse der Studie veranschaulichen die Lernprozesse einmal klassenübergreifend, zum anderen erfolgt eine detaillierte Analyse der einzelnen Lernenden. Das Literaturverzeichnis liefert Lehrenden, Studierenden und Forschern eine erschöpfende bibliographische Quelle zu den behandelten Themen.

Apprendimento implicito e esplicito delle lingue straniere interazione in classe costruire la competenza "parlare" in lingua tedesca analisi di videoregistrazioni in aula metodi intro- e retrospettiviFörderung von Mündlichkeit im DaF-Unterricht implizites und explizites Fremdsprachenlernen kooperatives Lernen Videographie qualitative Forschungsmethoden
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Gesti per pensare, pensieri per comunicare

2009

Apprendimento linguistico scuola lingue dei segni lingue verbali integrazione sviluppo del bambino relazione adulto-bambino bilinguismoSettore M-PED/01 - Pedagogia Generale E Sociale
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