Search results for "PREP"
showing 10 items of 1334 documents
A novel approach for analysis of oligonucleotide-cisplatin interactions by continuous elution gel electrophoresis coupled to isotope dilution inducti…
2008
In this work we present a novel approach for in vitro studies of cisplatin interactions with 8-mer oligonucleotides. The approach is based on the recently developed coupling of continuous elution gel electrophoresis (GE) to an inductively coupled plasma-sector field mass spectrometer (ICP-SFMS) with the aim of monitoring the interaction process between this cytostatic drug and the nucleotides. In contrast to existing methods, the electrophoretic separation conditions used here allow both the determination of the reaction kinetics in more detail as well as the observation of dominant intermediates. Two different nucleotides sequences have been investigated for comparison purposes, one contai…
Method development of levoglucosenone analysis by UHPLC-UV-MS in fast pyrolysis samples and aspects of its degradation in aqueous samples
2020
Levoglucosenone ((1S,5R)-6,8-dioxabicyclo[3.2.1]oct-2-en-4-one or LGO; CAS number: 37112-31-5) is an extensively studied biomass based chemical and its potential applications in organic synthesis, as well as in the extraction of various natural substances, have been established; therefore developing dependable, yet fast and simple analytical methods for determining LGO in the products of thermochemical biomass processing is of great importance. In this work different UHPLC methods have been developed and compared for the determination of LGO in wood pyrolysis products. In addition to UV detection, LGO determination conditions by single quadrupole mass spectrometry have been adjusted with an…
Analysis of fumonisins B1, B2 and B3 in corn-based baby food by pressurized liquid extraction and liquid chromatography/tandem mass spectrometry
2008
A sensitive and reliable method using pressurized liquid extraction (PLE) and liquid chromatography (LC)/electrospray ionization (ESI) tandem mass spectrometry with a triple quadrupole (QqQ) analyzer has been developed for the analysis of fumonisin B(1) (FB(1)), fumonisin B(2) (FB(2)) and fumonisin B(3) (FB(3)) in corn-based baby foods. Influence of several extraction parameters that affect PLE efficiency such as temperature, pressure, solvent extraction, number of cycles and dispersant/clean-up agents were studied. The selected PLE operating method was: 3g of sample was packed into 11 ml stainless-steel cell and fumonisins were extracted with methanol at 40 degrees C, 34 atm in one cycle o…
Comparison of various liquid chromatographic methods for the analysis of avermectin residues in citrus fruits.
2001
Abstract Various liquid chromatographic (LC) techniques for analyzing avermectin (Abamectin) were compared after extraction of residues from citrus fruit samples by matrix solid-phase dispersion (MSPD). LC with UV and fluorescence detection were used as also was LC coupled to the mass spectrometer by an electrospray interface. The results obtained by the three methods were compared in terms of sensitivity and selectivity. The combination of MSPD extraction and LC with fluorescence detection have made it possible to quantify 0.5 μg kg −1 of Abamectin in 0.5 g of orange sample, with an overall average recovery of 94%. The procedure provides a simple and sensitive method for monitoring Abamect…
Analysis of meat samples for anabolic steroids residues by liquid chromatography/tandem mass spectrometry.
2007
A rapid, specific and highly sensitive multi-residue method for the determination of anabolic steroid residues in bovine, pork and poultry muscle tissues was developed. The sample preparation involves enzymatic digestion followed by extraction with methanol. The crude extract was cleaned up by solid-phase extraction (SPE) combining C18 and NH2 columns. The detection was carried out by a highly sensitive liquid chromatography/electrospray ionization tandem mass spectrometry (LC/ESI-MS/MS) method using both positive and negative ionization modes. Natural and synthetic steroids covering different polarities could be extracted, concentrated and purified using one single method. Mobile phase com…
The role of community-based knowledge and local institutions in managing landslides on the slopes of Mount Elgon, Uganda
2015
The paper explores the degree to which community-based knowledge (CBK) and local institutions can be effective for disaster preparedness and management, based on a study carried out in Nametsi Parish, located on the slopes of Mount Elgon, in eastern Uganda. The study area has experienced innumerable landslides since 1933. Whereas a few of the landslides have not been disastrous, several others have had far-reaching effects on the livelihoods of people living in the area. This is attributed partly to the geographical setting, which makes the area difficult to access, and partly to socio-economic factors such as a high population growth rate and widespread poverty. Despite these aspects of vu…
VALIDITY, RELIABILITY AND FEASIBILITY OF MEASURING MUSCLE ACTIVITY WITH TEXTILE ELECTRODES EMBEDDED INTO CLOTHING
2007
INTRODUCTION In out-of-laboratory settings the surface EMG measurements are complicated by the requirement of careful electrode placement and skin preparation. The development of washable textile electrodes that can be sowed into sportswear enable muscle activity recordings during normal locomotion without skin preparation and the problem of wires hanging around the body [1]. In this study we performed validity, reliability and feasibility tests of the textile electrodes embedded into shorts against the traditional bipolar surface electrodes.
Assessment of competences in designing online preparatory materials for the Cambridge First Certificate in English examination
2012
The implementation of the competence-based approach set out by the Bologna Declaration within the European Higher Education Area (EHEA) entails important changes in learning processes and brings new approaches to content teaching and learning, inevitably affecting planning, methodology and assessment (Cano García, 2008). Such is the case of the design and development of the First Certificate in English Online Course & Tester at the Universidad Politécnica de Valencia (UPV) by the CAMILLE research group. These materials, published through InGenio1, are split into two parts: a preparatory course and a tester platform. This article explores some of the key competences to be included in these p…
Designing for Networked Community Resilience
2016
Abstract Communities have been described to be at the heart of the preparedness for and the response to disasters. The increasing connectedness has made communities more vulnerable for their dependence on a complex network of critical infrastructures. At the same time, this very connectedness has the potential to enable communities to self-organise, engage, and connect with other communities to improve their resilience. While the pathway to more resilience is promising and has many advocates, the response to crises and disasters, time and again reveals the challenges related to (i) ad-hoc switching from preparedness to response; (ii) ad-hoc connecting professional responders, communities, v…
Risk Accelerators in Disasters
2014
Published version of a chapter in the book: Advanced Information Systems Engineering. Also available from the publisher at: http://dx.doi.org/10.1007/978-3-319-07881-6_2 Modern societies are increasingly threatened by disasters that require rapid response through ad-hoc collaboration among a variety of actors and organizations. The complexity within and across today's societal, economic and environmental systems defies accurate predictions and assessments of damages, humanitarian needs, and the impact of aid. Yet, decision-makers need to plan, manage and execute aid response under conditions of high uncertainty while being prepared for further disruptions and failures. This paper argues tha…