Search results for "Particle Size"

showing 10 items of 631 documents

Medium-size droplets of methyl ricinoleate are reduced by cell-surface activity in the gamma-decalactone production by Yarrowia lipolytica.

2000

International audience; Size of methyl ricinoleate droplets during biotransformation into gamma-decalactone by Yarrowia lipolytica was measured in both homogenized and non-homogenized media. In non-homogenized but shaken medium, droplets had an average volume surface diameter d32 of 2.5 microm whereas it was 0.7 microm in homogenized and shaken medium. But as soon as yeast cells were inoculated, both diameters became similar at about 0.7 microm and did not vary significantly until the end of the culture. The growth of Y. lipolytica in both media was very similar except for the lag phase which was lowered in homogenized medium conditions.

0106 biological sciences[SDV.BIO]Life Sciences [q-bio]/BiotechnologyTime FactorsCell01 natural sciencesApplied Microbiology and BiotechnologyLactonesBiotransformationMESH : Particle SizeYeastsMESH: Microscopy Confocal[INFO.INFO-BT]Computer Science [cs]/BiotechnologyComputingMilieux_MISCELLANEOUSBiotransformation0303 health sciencesMicroscopyMicroscopy ConfocalbiologyMESH: YeastsMESH : Lactones[SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitologymedicine.anatomical_structureBiochemistryConfocalSURFACE ACTIVERicinoleic Acids[ INFO.INFO-BT ] Computer Science [cs]/BiotechnologyMESH: LactonesMESH : Time Factors03 medical and health sciencesMESH : Biotransformation010608 biotechnologymedicine[SDV.BBM] Life Sciences [q-bio]/Biochemistry Molecular Biology[SDV.BBM]Life Sciences [q-bio]/Biochemistry Molecular BiologyMESH: Particle SizeParticle SizeMESH : Microscopy Confocal[ SDV.BBM ] Life Sciences [q-bio]/Biochemistry Molecular BiologyMethyl ricinoleateMESH: BiotransformationMESH : YeastsChromatography030306 microbiologyMESH: Time Factors[ SDV.BIO ] Life Sciences [q-bio]/BiotechnologyYarrowiabiology.organism_classificationYeastMESH: Ricinoleic AcidsCulture Media[SDV.BIO] Life Sciences [q-bio]/Biotechnology[INFO.INFO-BT] Computer Science [cs]/BiotechnologyMESH : Ricinoleic AcidsMESH: Culture MediaMESH : Culture Media
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The effects of different combinations of fixed and moving bed bioreactors on rainbow trout (Oncorhynchus mykiss) growth and health, water quality and…

2019

Abstract The effect of bioreactor design on nitrification efficiency has been well studied, but less is known about the overall impacts on water quality. Besides nitrification, submerged fixed bed bioreactors (FBBR) trap fine solid particles, whereas moving bed bioreactors (MBBR) grind solids, possibly increasing solids and particle accumulation in the system. In this experiment, the effects of different combinations of fixed bed and moving bed bioreactors on water quality, solids removal, particle size distribution, fish health based on histopathological changes and nitrification efficiency were studied in laboratory scale recirculating aquaculture systems (RAS) with rainbow trout (Oncorhy…

0106 biological sciencesbiofiltrationwater quality monitoringnitrifikaatioAquatic Science01 natural scienceslaw.inventionchemistry.chemical_compoundlawBioreactorparticle size distributionNitritevesiviljely (kalatalous)Filtrationta218ta415010604 marine biology & hydrobiologyta1183technology industry and agriculture04 agricultural and veterinary sciencesPulp and paper industryTotal dissolved solidsvedenlaatuFilter (aquarium)taudinaiheuttajatchemistrybioreaktoritBiofilter040102 fisherieshistopathology0401 agriculture forestry and fisheriesNitrificationWater qualitysuodatusAquacultural Engineering
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Spray-Drying Performance and Thermal Stability of L-Ascorbic Acid Microencapsulated with Sodium Alginate and Gum Arabic

2019

[EN] The potential of sodium alginate (ALG) and gum arabic (GA) as wall polymers for Lascorbic acid (AA) encapsulation as a tool for their preservation against the thermo-oxidative degradation was investigated. The influence of such polymers used as wall material on the AAcontent, size, encapsulation efficiency, encapsulation yield and thermo-oxidative stability were evaluated. The AA-microparticles were obtained using the spray-drying technique. An experimental Taguchi design was employed to assess the influence of the variables in the encapsulation process. The microparticles morphology and size distribution were characterized by scanning electron microscopy and laser diffraction. The the…

0106 biological sciencesfood.ingredientMaterials scienceChemical PhenomenaScanning electron microscopeAlginatesDrug Compoundinggum arabicPharmaceutical ScienceAscorbic AcidL-ascorbic acid01 natural sciencesArticleAnalytical Chemistrysodium alginatelcsh:QD241-4410404 agricultural biotechnologyfoodDifferential scanning calorimetryDrug Stabilitylcsh:Organic chemistry010608 biotechnologyDrug DiscoveryThermal stabilityspray-dryingPhysical and Theoretical ChemistryParticle Sizechemistry.chemical_classificationAnalysis of VarianceMolecular StructurenanotechnologySpectrum AnalysisOrganic ChemistryTemperature04 agricultural and veterinary sciencesPolymerAscorbic acid040401 food scienceThermogravimetrychemistryChemical engineeringChemistry (miscellaneous)Spray dryingMAQUINAS Y MOTORES TERMICOSMolecular MedicineGum arabicencapsulation
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Regarding the influence of the particle size of crumb rubber from waste rubber on the physical and mechanical characteristics of reclaimed rubber

2017

Thus, at present, a large amount of rubber waste cannot be higher harnessed due to recovery technologies used. Also, the requirements for new products of rubber worldwide are greater than the production capacity and the availability of raw materials. To offset this balance is necessary to improve technologies for recycling waste rubber so to can get regenerated rubber with the best mechanical physical characteristics. The main technology for obtaining the regenerated rubber is based on the rubber waste shredding and making the powder, so that it can be carried out de-vulcanization in autoclave. All the time it was monitored as the de-vulcanization process to be as economical and less pollut…

020502 materials020209 energy02 engineering and technologyRaw materialPulp and paper industryAutoclave0205 materials engineeringNatural rubberlcsh:TA1-2040visual_art0202 electrical engineering electronic engineering information engineeringvisual_art.visual_art_mediumParticleCrumb rubberParticle sizeWaste rubberComposite materiallcsh:Engineering (General). Civil engineering (General)MATEC Web of Conferences
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Effect of heating method on the microbial levels and acrylamide in corn grits and subsequent use as functional ingredient for bread making

2018

Abstract Cereal by-products represent abundant and low-cost resources of nutrients. The study is dedicated to the development of a rapid method for the treatment of corn grits to obtain value added ingredients for breadmaking. The infrared irradiation (IR) at 76 °C and 90 °C and vacuum microwave (VM) heating at 60 °C have been applied for stabilization of corn grits. The corn grit particles were fractionated by size and it was found that the particle size fraction significantly (p

0301 basic medicine030109 nutrition & dieteticsGeneral Chemical EngineeringFraction (chemistry)04 agricultural and veterinary sciences040401 food scienceBiochemistryInfrared irradiation03 medical and health sciencesIngredientchemistry.chemical_compound0404 agricultural biotechnologyNutrientchemistryAcrylamideParticle sizeFood scienceBread makingFood ScienceBiotechnologyFood and Bioproducts Processing
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Hybrid Biopolymer and Lipid Nanoparticles with Improved Transfection Efficacy for mRNA

2020

Cells 9(9), 2034 (1-19) (2020). doi:10.3390/cells9092034

0301 basic medicine570small angle scatteringNanoparticlecationic lipid02 engineering and technologyengineering.materialTransfectionArticleCell LineFatty Acids Monounsaturated03 medical and health sciencesMiceBiopolymersddc:570AnimalsHumansRNA MessengerParticle Sizelcsh:QH301-705.5chemistry.chemical_classificationMice Inbred BALB Ccancer immunotherapySmall-angle X-ray scatteringHeparinOptical ImagingCationic polymerizationGeneral MedicinePolymerTransfection021001 nanoscience & nanotechnologyvaccinationSmall-angle neutron scatteringLipidslipid-polymer hybrid nanoparticlesQuaternary Ammonium Compounds030104 developmental biologychemistrylcsh:Biology (General)cationic polymerBiophysicsengineeringNanoparticlesRNAFemalePolymer blendBiopolymer0210 nano-technologyCovid-19
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Physicochemical and Preclinical Evaluation of Spermine-Derived Surfactant Liposomes for in Vitro and in Vivo siRNA-Delivery to Liver Macrophages

2016

Herein we report on a liposomal system for siRNA delivery consisting of cholesterol (Chol), distearoylphosphatidylcholine (DSPC), and surfactant TF (1-hydroxy-50-amino-3,4,7,10,13,16,19,22-octaoxa-37,41,45-triaza-pentacontane), a novel spermine derivative (HO-EG8-C12-spermine) which has shown improved siRNA delivery to cells in vitro and in vivo. Predominantly single-walled liposomes with reproducible sizes and moderately broad size distributions were generated with an automated extrusion device. The liposomes remained stable when prepared in the presence of siRNA at N/P ratios of 17-34. However, when mixed with human serum in equal volumes, larger aggregates in the size range of several hu…

0301 basic medicineAntigens Differentiation MyelomonocyticPharmaceutical ScienceSpermineFlow cytometryMiceSurface-Active Agents03 medical and health scienceschemistry.chemical_compoundDynamic light scatteringPulmonary surfactantAntigens CDIn vivoDrug DiscoverymedicineAnimalsParticle SizeRNA Small InterferingCells CulturedDrug CarriersLiposomemedicine.diagnostic_testReverse Transcriptase Polymerase Chain ReactionMacrophagesModels TheoreticalFlow CytometryIn vitroCholesterol030104 developmental biologyLiverchemistryBiochemistryLiposomesPhosphatidylcholinesMolecular MedicineSpermineDrug carrierMolecular Pharmaceutics
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Biosynthesis of selenium-nanoparticles and -nanorods as a product of selenite bioconversion by the aerobic bacterium Rhodococcus aetherivorans BCP1

2018

The wide anthropogenic use of selenium compounds represents the major source of selenium pollution world- wide, causing environmental issues and health concerns. Microbe-based strategies for metal removal/recovery have received increasing interest thanks to the association of the microbial ability to detoxify toxic metal/ metalloid polluted environments with the production of nanomaterials. This study investigates the tolerance and the bioconversion of selenite (SeO32−) by the aerobically grown Actinomycete Rhodococcus aetherivorans BCP1 in association with its ability to produce selenium nanoparticles and nanorods (SeNPs and SeNRs). The BCP1 strain showed high tolerance towards SeO32− with…

0301 basic medicineBioconversionStatic Electricity030106 microbiologychemistry.chemical_elementBioengineeringSelenious AcidSettore BIO/19 - Microbiologia GeneraleSelenium pollutionSelenium03 medical and health sciencesMinimum inhibitory concentrationchemistry.chemical_compoundNanoparticleBiosynthesisRhodococcusParticle SizeSelenite Rhodococcus aetherivorans Selenium nanoparticles Selenium nanorods Biogenic nanostructuresSelenium nanorodMolecular BiologyNanotubesbiologyBiogenic nanostructureRhodococcus aetherivoranSpectrometry X-Ray EmissionGeneral Medicinebiology.organism_classificationDynamic Light ScatteringSelenium nanoparticleBacteria AerobicNanotube030104 developmental biologychemistryBiochemistry13. Climate actionSelenious AcidSeleniteNanoparticlesMetalloidRhodococcusSeleniumRhodococcuBiotechnologyNew Biotechnology
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Dextran-based therapeutic nanoparticles for hepatic drug delivery.

2016

Aim: Evaluation of dextran-based nanoparticles (DNP) as a drug delivery system to target myeloid cells of the liver. Materials & methods: DNP were synthesized and optionally PEGylated. Their toxicity and cellular uptake were studied in vitro. Empty and siRNA-carrying DNP were tested in vivo with regard to biodistribution and cellular uptake. Results: In vitro, DNP were taken up by cells of the myeloid lineage without compromising their viability. In vivo, empty and siRNA-carrying DNP distributed to the liver where a single treatment addressed approximately 70% of macrophages and dendritic cells. Serum parameters indicated no in vivo toxicity. Conclusion: DNP are multifunctional liver-s…

0301 basic medicineBiodistributionMaterials scienceCell SurvivalSurface PropertiesBiomedical EngineeringMedicine (miscellaneous)Antigens Differentiation Myelomonocyticchemical and pharmacologic phenomenaBioengineering02 engineering and technologyDevelopmentPharmacologyPolyethylene Glycols03 medical and health scienceschemistry.chemical_compoundMiceIn vivoAntigens CDAnimalsHumansGeneral Materials ScienceTissue DistributionParticle SizeRNA Small InterferingDrug CarriersMice Inbred BALB Corganic chemicalsMacrophageshemic and immune systemsDextransDendritic cell3T3 CellsDendritic Cells021001 nanoscience & nanotechnology030104 developmental biologyDextranRAW 264.7 CellschemistryLiverDrug deliveryToxicityPEGylationNanoparticles0210 nano-technologyDrug carrierNanomedicine (London, England)
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Skin-derived mesenchymal stem cells as quantum dot vehicles to tumors

2017

Dominyka Dapkute,1,2 Simona Steponkiene,1 Danute Bulotiene,1 Liga Saulite,3 Una Riekstina,3 Ricardas Rotomskis1,4 1Biomedical Physics Laboratory, National Cancer Institute, Vilnius, Lithuania; 2Institute of Biosciences, Vilnius University, Vilnius, Lithuania; 3Faculty of Medicine, University of Latvia, Riga, Latvia; 4Biophotonics Group of Laser Research Center, Faculty of Physics, Vilnius University, Vilnius, Lithuania Purpose: Cell-mediated delivery of nanoparticles is emerging as a new method of cancer diagnostics and treatment. Due to their inherent regenerative properties, adult mesenchymal stem cells (MSCs) are naturally attracted to wounds and sites of inflammation, as well as tumors.…

0301 basic medicineBiophysicsPharmaceutical ScienceConnective tissueBioengineeringBreast Neoplasmsquantum dotsMice SCIDFlow cytometryBiomaterialsCell therapy03 medical and health sciencesIn vivoCell MovementInternational Journal of NanomedicineCell Line TumorDrug DiscoverymedicineAnimalsHumansViability assayParticle SizeCytotoxicityCell ShapeSkinOriginal Researchmesenchymal stem cellsMigration Assaymedicine.diagnostic_testCell DeathChemistryOrganic ChemistryMesenchymal stem cellGeneral MedicineDynamic Light ScatteringEndocytosis030104 developmental biologymedicine.anatomical_structureimmunodeficient miceCancer researchNanoparticlesFemaletumor tropismtumor-specific deliveryInternational Journal of Nanomedicine
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