Search results for "Pf"
showing 10 items of 526 documents
Structural and Functional Analysis of BBA03, Borrelia burgdorferi Competitive Advantage Promoting Outer Surface Lipoprotein
2020
BBA03 is a Borrelia burgdorferi outer surface lipoprotein encoded on one of the most conserved plasmids in Borrelia genome, linear plasmid 54 (lp54). Although many of its genes have been identified as contributing or essential for spirochete fitness in vivo, the majority of the proteins encoded on this plasmid have no known function and lack homologs in other organisms. In this paper, we report the solution NMR structure of the B. burgdorferi outer surface lipoprotein BBA03, which is known to provide a competitive advantage to the bacteria during the transmission from tick vector to mammalian host. BBA03 shows structural homology to other outer surface lipoproteins reflecting their genetic …
Salmonella bongori48:z35:– in Migratory Birds, Italy
2009
Suggested citation for this article: Foti M, Daidone A, Aleo A, Pizzimenti A, Giacopello C, Mammina C. Salmonella bongori 48:z35:– in migratory birds, Italy. Emerg Infect Dis [serial on the Internet]. 2009 Mar [date cited]. Available from http://www.cdc.gov/EID/content/15/3/502.htm
Characterization of Listeria monocytogenes isolates from human listeriosis cases in Italy.
2009
ABSTRACT The objective of this study was to characterize by serotyping, pulsed-field gel electrophoresis (PFGE), and PCR amplification of virulence genes and markers of epidemic clones I, II, and III (ECI, ECII, and ECIII) 54 human isolates from apparently sporadic cases of infection occurring in the Lombardy region and in the province of Florence, Tuscany, Italy, in the years 1996 to 2007. Listeria monocytogenes isolates were provided by the clinical microbiology laboratories of the Lombardy region and the “Careggi” Hospital of Florence, Tuscany, Italy. Serotyping, PFGE after digestion with the AscI and ApaI enzymes, and PCR amplification for the inlA , inlC , and inlJ genes and ECI, ECII,…
Electrical Pumping of Potassium Ions Against an External Concentration Gradient in a Biological Ion Channel
2013
We show experimentally and theoretically that significant currents can be obtained with a biological ion channel, the OmpF porin of Escherichia coli, using zero-average potentials as driving forces. The channel rectifying properties can be used to pump potassium ions against an external concentration gradient under asymmetric pH conditions. The results are discussed in terms of the ionic selectivity and rectification ratio of the channel. The physical concepts involved may be applied to separation processes with synthetic nanopores and to bioelectrical phenomena. (C) 2013 AIP Publishing LLC.
Bioaccumulation of emerging contaminants in mussel (Mytilus galloprovincialis): Influence of microplastics
2021
Coastal environments are heavily influenced by human activities. Chemical substances considered as emerging contaminants (ECs) are one of the most important indicators of the anthropic influence on the environment, and they have recently shown to interact with microplastics (MPs). Mussels are suitable for in-lab bioacumulation studies providing insight about the occurrence and fate of contaminants in the organisms. In this study, bioacummulation of 20 chemical substances catalogued as ECs, including pharmaceuticals and personal care products (PPCPs), pesticides, and perfluoroalkyl substances (PFASs) in Mytilus galloprovincialis was assessed, with or without the influence of the presence of …
The non-bilayer lipid MGDG stabilizes the major light-harvesting complex (LHCII) against unfolding.
2017
Abstract In the photosynthetic apparatus of plants a high proportion of LHCII protein is needed to integrate 50% non-bilayer lipid MGDG into the lamellar thylakoid membrane, but whether and how the stability of the protein is also affected is not known. Here we use single-molecule force spectroscopy to map the stability of LHCII against mechanical unfolding along the polypeptide chain as a function of oligomerization state and lipid composition. Comparing unfolding forces between monomeric and trimeric LHCII demonstrates that the stability does not increase significantly upon trimerization but can mainly be correlated with specific contact sites between adjacent monomers. In contrast, unfol…
Folding and stability of the aquaglyceroporin GlpF: Implications for human aqua(glycero)porin diseases
2015
AbstractAquaporins are highly selective polytopic transmembrane channel proteins that facilitate the permeation of water across cellular membranes in a large diversity of organisms. Defects in aquaporin function are associated with common diseases, such as nephrogenic diabetes insipidus, congenital cataract and certain types of cancer. In general, aquaporins have a highly conserved structure; from prokaryotes to humans. The conserved structure, together with structural dynamics and the structural framework for substrate selectivity is discussed. The folding pathway of aquaporins has been a topic of several studies in recent years. These studies revealed that a conserved protein structure ca…
Noziedzīgi iegūtu līdzekļu legalizācijas novēršanas pasākumi Latvijas finanšu sistēmā
2022
Noziedzīgi iegūto līdzekļu legalizācija un terorisma un proliferācijas finansēšanas jēdziens finanšu sistēmā kļūst aizvien atpazīstamāks, balstoties uz 21. gadsimtā noziegumu skaitu palielināšanos un cīņai pret to vērsto darbību un mehānismu attīstību. Mūsdienās ikviens cilvēks vēlas dzīvot drošā vidē, un cīņa pret noziedzīgi iegūto līdzekļu legalizāciju un terorisma un proliferāciju ir viena no sastāvdaļām, ar kurām ir iespējams samazināt noziegumu skaitu. Kredītiestādes ir vairāk pakļautas tikt iejauktām un izmantotām noziedzīgi iegūto līdzekļu legalizācijā, līdz ar to, no likumdošanas puses, tām tiek piemēroti striktāki noteikumi, kas rada ne tikai NILLTFPN sistēmas stiprināšanu, bet arī…
Modulation of drug transport by selected flavonoids: Involvement of P-gp and OCT?
2004
Flavonoids, as a common component of daily nutrition, are a possible source of interference with absorption processes, due to modulation of transporting proteins. In this study, the influence of selected flavonoids (quercetin, isoquercitrin, spiraeoside, rutin, kaempferol, naringenin, naringin, and kaempferol) on the transport of the P-gp substrate [3H]talinolol across Caco-2 cell monolayers was investigated. To elucidate the mechanism behind the interaction observed in this system the potency of the flavonoids to replace [3H]talinolol from its P-gp binding site as well as their activity to inhibit OCT2-mediated [14C]TEA uptake into LLC-PK(1) cells were measured, as P-gp and OCT have been s…
Influence of anti-inflammatory flavonoids on degranulation and arachidonic acid release in rat neutrophils.
1994
We assessed the effects of 24 flavonoid derivatives, reported as anti-inflammatory, on lysosomal enzyme secretion and arachidonic acid release in rat neutrophils. Amentoflavone, quercetagetin- 7-O -glucoside, apigenin, fisetin, kaem pferol, luteolin and quercetin were the most potent inhibitors of β-glucuronidase and lysozyme release. The first com pound was also able to inhibit basal release. These flavonoids besides chrysin and to a reduced extent, naringenin, significantly inhibited arachidonic acid release from membranes. A correlation between degranulation and arachidonic acid release was found for this series of compounds. Structureactivity relationships and implications for the anti-…