Search results for "Phenotype"

Stress-controlled transcription factors, stress-induced genes and stress tolerance in budding yeast.

2000

The transcriptional response to environmental changes is a major topic in both basic and applied research. From a basic point of view, to understand this response includes unravelling how the stress signal is sensed and transduced to the nucleus, to identify which genes are induced under each stress condition and, finally, to establish the phenotypic consequences of this induction in stress tolerance. The possibility of using genetic approaches has made the yeast Saccharomyces cerevisiae a compelling model to study stress response at a molecular level. Moreover, this information can be used to isolate and characterise stress-related proteins in higher eukaryotes and to design strategies to …

Major gene effects during weed evolution: phenotypic characters cosegregate with alleles at the ray floret locus in Senecio vulgaris L. (Asteraceae)

1998

Characterization of a partial exon 9/intron 9 deletion in the coagulation factor XII gene (F12) detected in two Turkish families with hereditary angi…

2014

Flow Cytometry and Karyotype Analysis ofD. melanogasterEye Disc Cells

2008

The developing Drosophila eye-antennal disc is a particularly suited system for the genetic and cellular studies of complex biological processes. Methods to analyze Drosophila eye discs by flow cytometry are mainly based on the dissociation of tissues with trypsin. Dissociation operated by trypsin is very effective, though it causes a lot of stress to live cells often compromising the use of treated cells for further analyses. Here, we report a method to produce dissociated eye-disc cells that retain cell-membrane markers and that can be used for flow cytometry and cytological analysis of mitotic chromosomes. The method described is a great complementing tool for the cellular characterizati…

Corsello G, Maresi E, Corrao AM, Dimita U, Lo Cascio M, Cammarata M, Giuffrè L (1992): VATER/VACTERL association: Clinical variability and expanding …

1993

Genetic basis of trichome production in Arabidopsis lyrata.

2002

Leaf trichomes may protect plants against herbivorous insects, and may increase tolerance to drought and UV-radiation. The perennial herb Arabidopsis lyrata (Brassicaceae) is polymorphic for trichome production and occurs in a glabrous and trichome-producing form. In addition, there is quantitative variation in trichome density among trichome-producing plants. To examine the genetic basis of glabrousness, we conducted controlled crosses with plants originating from two natural populations in Sweden (one polymorphic for trichome-production, and one consisting of glabrous plants only). In addition, we estimated the heritability of trichome number from parent-offspring regressions for plants o…

Specific and promiscuous functions of multiple DnaJ proteins in Synechocystis sp. PCC 6803

2011

Cyanobacterial genomes typically encode multiple Hsp70 (DnaK) and Hsp40 (DnaJ) chaperones, and in the genome of the cyanobacteriumSynechocystisPCC 6803, three DnaK proteins are encoded together with seven DnaJ proteins. While only two of the DnaJ proteins can complement the growth defect of anEscherichia coliΔdnaJstrain, only disruption of thednaJgenesll0897resulted in a growth defect at elevated temperatures. Based on the domain structure and the phenotype observed following disruption of the encoding gene, Sll0897 can be classified as a canonical heat-shock protein inSynechocystis. Furthermore, mostdnaJgenes could be deleted individually, whereas disruption of the gene encoding the DnaJ S…

Transgenic Expression of a Toxin-Coding Killer Virus of the Yeast Zygosaccharomyces bailii in Saccharomyces cerevisiae: Genetic Evidence for a Possib…

1996

The killer toxin-secreting yeast Zygosaccharomyces bailii 412 contains two cytoplasmically inherited double-stranded RNA (dsRNA) viruses (ZbV-L, ZbV-M) responsible for the expression of a killer phenotype in its infected host. While ZbV-L functions as a classical helpervirus by providing capsid (cap) and RNA polymerase functions (cap/pol) necessary for packaging and replication of both viruses, M-dsRNA-containing killer viruses (ZbV-M) are satellites of ZbV-L that contain the genetic information for toxin production only. Both viruses were shown to be sufficient to confer the Z. bailii killer phenotype upon transfected spheroplasts of a S. cerevisiae non-killer strain, resulting in toxin-se…

Metabolic Networks of Sodalis glossinidius: A Systems Biology Approach to Reductive Evolution

2012

BackgroundGenome reduction is a common evolutionary process affecting bacterial lineages that establish symbiotic or pathogenic associations with eukaryotic hosts. Such associations yield highly reduced genomes with greatly streamlined metabolic abilities shaped by the type of ecological association with the host. Sodalis glossinidius, the secondary endosymbiont of tsetse flies, represents one of the few complete genomes available of a bacterium at the initial stages of this process. In the present study, genome reduction is studied from a systems biology perspective through the reconstruction and functional analysis of genome-scale metabolic networks of S. glossinidius.ResultsThe functiona…

Simultaneous Aurora-A/STK15 overexpression and centrosome amplification induce chromosomal instability in tumour cells with a MIN phenotype

2007

Abstract Background Genetic instability is a hallmark of tumours and preneoplastic lesions. The predominant form of genome instability in human cancer is chromosome instability (CIN). CIN is characterized by chromosomal aberrations, gains or losses of whole chromosomes (aneuploidy), and it is often associated with centrosome amplification. Centrosomes control cell division by forming a bipolar mitotic spindle and play an essential role in the maintenance of chromosomal stability. However, whether centrosome amplification could directly cause aneuploidy is not fully established. Also, alterations in genes required for mitotic progression could be involved in CIN. A major candidate is represe…