Search results for "Pichia Pastoris"

showing 3 items of 13 documents

A rapid method for the differentiation of yeast cells grown under carbon and nitrogen-limited conditions by means of partial least squares discrimina…

2012

This paper shows the ease of application and usefulness of mid-IR measurements for the investigation of orthogonal cell states on the example of the analysis of Pichia pastoris cells. A rapid method for the discrimination of entire yeast cells grown under carbon and nitrogen-limited conditions based on the direct acquisition of mid-IR spectra and partial least squares discriminant analysis (PLS-DA) is described. The obtained PLS-DA model was extensively validated employing two different validation strategies: (i) statistical validation employing a method based on permutation testing and (ii) external validation splitting the available data into two independent sub-sets. The Variable Importa…

Time FactorsChemistry(all)Spectrophotometry InfraredNitrogenAnalytical chemistryInfrared spectroscopyPichiaArticleAnalytical ChemistryPichia pastorisPichia pastorisInfrared (IR) micro-spectroscopyPartial least squares regressionProcess controlPartial least squares-discriminant analysis (PLS-DA)Least-Squares AnalysisProjection (set theory)Cell ProliferationPrincipal Component AnalysisbiologyChemistryDiscriminant AnalysisReproducibility of ResultsLinear discriminant analysisbiology.organism_classificationDouble cross validation (2CV)YeastCarbonYeastCulture MediaPermutation testingPrincipal component analysisFeasibility StudiesBiological systemTalanta
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Function of odorant-binding proteins in the Drosophila melanogaster chemoreception

2017

National audience; Function of odorant-binding proteins in the Drosophila [i]melanogaster[/i] chemoreception. 18. rencontre du Club de neurobiologie des invertébrés

pichia pastorisanimal structures[ SDV.AEN ] Life Sciences [q-bio]/Food and Nutritioneducationodorant-binding proteinsdrosophila melanogasterhumanitiestestingCAFÉ assay[SDV.AEN] Life Sciences [q-bio]/Food and Nutritionprotéineessaiprotein[SDV.AEN]Life Sciences [q-bio]/Food and Nutritionhuman activitiespsychological phenomena and processeshealth care economics and organizationsfluorescent binding assays
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Melanokortīna otrā tipa receptora un tā palīgproteīna ekspresija rauga Pichia Pastoris ekspresijas sistēmā

2015

Darba ietvaros tika izveidots pPIC9K- MC2R-T4L ekspresijas konstrukts, kas tika ekspresēts P.Pastoris sistēmā. Western blot analīze uzrādīja nepieciešamās molekulmasas zonu. MC2R-T4L izdevās attīrīt uz His- un Ha-tag afinitātes kolonnām. Lai noteiktu funkcionāla MC2R struktūru, to nepiecieciešams ekspresēt kompleksā ar MRAP, tādēļ tika izveidoti vairāki MC2-MRAP konstrukti, ko tālāk izmantot proteīna ekspresijai P. Pastoris šūnās. To aktivitāte tika pārbaudīta zīdītāju šūnu līnijās nosakot cAMP daudzuma izmaiņas pēc stimulācijas ar ACTH (1-24). Iegūtie rezultāti bija nestabili, tādēļ tālākajos pētījumos tiks veikta radioaktīvā ACTH saistīšanas mērījumus. Darbs tika veikts no 2013.gada jūnij…

proteīnsekspresijas sistēmaMC2RBioloģijaMRAPPichia Pastoris
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