Search results for "Platelets"

showing 10 items of 218 documents

In vitro and in vivo characterization of a new organic nitrate hybrid drug covalently bound to pioglitazone.

2014

<b><i>Background/Aims:</i></b> Organic nitrates represent a group of nitrovasodilators that are clinically used for the treatment of ischemic heart disease. The new compound CLC-3000 is an aminoethyl nitrate (AEN) derivative of pioglitazone, a thiazolidinedione antidiabetic agent combining the peroxisome proliferator-activated receptor γ agonist activity of pioglitazone with the NO-donating activity of the nitrate moiety. <b><i>Methods:</i></b> In vitro and in vivo characterization was performed by isometric tension recording, platelet function, bleeding time and detection of oxidative stress. <b><i>Results:</i></…

DrugBlood PlateletsMaleBleeding TimePlatelet Aggregationmedia_common.quotation_subjectVasodilator Agentsmedicine.disease_causeMitochondria Heartchemistry.chemical_compoundNitrateFibrinolytic AgentsIn vivomedicineAnimalsHumansHypoglycemic AgentsRats WistarAortamedia_commonPharmacologyNitratesPioglitazoneChemistryGeneral MedicineReactive Nitrogen SpeciesIn vitroOrganic nitratesMice Inbred C57BLVasodilationBiochemistryCovalent bondVasoconstrictionThiazolidinedionesReactive Oxygen SpeciesPioglitazoneOxidative stressmedicine.drugPharmacology
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COVID-19 disease - Temporal analyses of complete blood count parameters over course of illness, and relationship to patient demographics and manageme…

2020

Background Detailed temporal analyses of complete (full) blood count (CBC) parameters, their evolution and relationship to patient age, gender, co-morbidities and management outcomes in survivors and non-survivors with COVID-19 disease, could identify prognostic clinical biomarkers. Methods From 29 January 2020 until 28 March 2020, we performed a longitudinal cohort study of COVID-19 inpatients at the Italian National Institute for Infectious Diseases, Rome, Italy. 9 CBC parameters were studied as continuous variables [neutrophils, lymphocytes, monocytes, platelets, mean platelet volume, red blood cell count, haemoglobin concentration, mean red blood cell volume and red blood cell distribu…

Erythrocyte IndicesMaleViral DiseasesNeutrophilsPhysiologyclinical biomarkersRomeDisease030204 cardiovascular system & hematologyCardiovascular MedicineCohort StudiesLeukocyte CountWhite Blood Cells0302 clinical medicineMedical ConditionsAnimal CellsMedicine and Health SciencesRenal Failure030212 general & internal medicineLongitudinal StudiesLymphocytesSurvivorsCOPDMultidisciplinarymedicine.diagnostic_testQRComplete blood countMiddle AgedPrognosisBody FluidsInfectious DiseasesBloodSettore MED/38 - PEDIATRIA GENERALE E SPECIALISTICAPhysiological Parameterscovid-19; blood cell count; clinical biomarkerscovid-19Cardiovascular DiseasesNephrologyMedicineFemaleCellular TypesAnatomyMean Platelet VolumeCohort studyResearch ArticlePlateletsmedicine.medical_specialtyScienceImmune CellsImmunologyCardiology03 medical and health sciencesInternal medicinemedicineHumansObesityMean platelet volumeDemographyInflammationBlood Cellsbusiness.industryBody WeightBiology and Life SciencesRed blood cell distribution widthCovid 19Cell Biologymedicine.diseaseObesityBlood CountsAnisocytosisblood cell countbusinessBiomarkers
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Graphene coating obtained in a cold-wall CVD process on the Co-Cr Alloy (L-605) for medical applications

2021

Graphene coating on the cobalt-chromium alloy was optimized and successfully carried out by a cold-wall chemical vapor deposition (CW-CVD) method. A uniform layer of graphene for a large area of the Co-Cr alloy (discs of 10 mm diameter) was confirmed by Raman mapping coated area and analyzing specific G and 2D bands

ErythrocytesMicroscopeScanning electron microscope02 engineering and technologyChemical vapor deposition01 natural scienceslaw.inventionlcsh:ChemistryMiceCoated Materials BiocompatibleCoatinglawMaterials TestingComposite materiallcsh:QH301-705.5SpectroscopySettore CHIM/02 - Chimica Fisicagraphene coating ; biocompatibility ; cobalt chromium alloy ; cold wall chemical vapor deposition methodGeneral Medicine021001 nanoscience & nanotechnologyMicrostructureBlood Coagulation FactorsComputer Science ApplicationsGraphitePartial Thromboplastin TimeBiocompatibility0210 nano-technologyLayer (electronics)Blood PlateletsMaterials scienceCell SurvivalSurface PropertiesPrimary Cell Cultureengineering.material010402 general chemistryCobalt-chromium alloyGraphene coatingCold-wall chemical vapor deposition methodArticleCatalysisInorganic ChemistryAnimalsHumansPhysical and Theoretical ChemistryMolecular BiologyGrapheneOrganic Chemistrytechnology industry and agricultureNanoindentationPlatelet Activation0104 chemical scienceslcsh:Biology (General)lcsh:QD1-999NIH 3T3 CellsengineeringChromium AlloysVolatilization
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Colloidal Nanoplatelet/Conducting Polymer Hybrids: Excitonic And Material Properties

2016

WOS:000370678700053 Here we present the first account of conductive polymer/colloidal nanoplatelet hybrids. For this, we developed DEH-PPV-based polymers with two different anchor groups (sulfide and amine) acting as surfactants for CdSe nanoplatelets, which are atomically flat semiconductor nanocrystals. Hybridization of the polymers with the nanoplatelets in the solution phase was observed to cause strong photoluminescence quenching in both materials. Through steady-state photoluminescence and excitation spectrum measurements, photoluminescence quenching was shown to result from dominant exciton dissociation through charge transfer at the polymer/nanoplatelet interfaces that possess a sta…

Excitation spectrumMaterials sciencePhotoluminescenceSulfideDEH-PPV-Based PolymersSulfideExcitonCdSe NanoplateletsNanotechnology02 engineering and technologySurface active agents010402 general chemistryOptoelectronic devices01 natural sciencesDissociation (chemistry)ColloidCharge transferQuenchingHybrid optoelectronic devicesPhysical and Theoretical ChemistryPhotoluminescenceSulfur compoundsAmineSemiconductor nanocrystalsConductive polymerchemistry.chemical_classificationExcited statesBuilding blockesPolymerInterface statesEmission quenching021001 nanoscience & nanotechnology0104 chemical sciencesSurfaces Coatings and FilmsElectronic Optical and Magnetic MaterialsPolymer/Colloidal Nanoplatelet HybridsGeneral EnergyChemical engineeringchemistryExcited stateAnchorsExcitons0210 nano-technologyDissociationConductive polymerPhotoluminescence quenchingExciton dissociation
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Ultrastructure of the Interlamellar Membranes of the Nacre of the Bivalve Pteria hirundo, Determined by Immunolabelling.

2015

The current model for the ultrastructure of the interlamellar membranes of molluscan nacre imply that they consist of a core of aligned chitin fibers surrounded on both sides by acidic proteins. This model was based on observations taken on previously demineralized shells, where the original structure had disappeared. Despite other earlier claims, no direct observations exist in which the different components can be unequivocally discriminated. We have applied different labeling protocols on non-demineralized nacreous shells of the bivalve Pteria. With this method, we have revealed the disposition and nature of the different fibers of the interlamellar membranes that can be observed on the …

In situPlateletsBivalvesScanning electron microscopeShell (structure)Mineralogylcsh:MedicineChitinMatrix (biology)chemistry.chemical_compoundChitinAnimal ShellsMembrane proteinsAnimalsFiberlcsh:ScienceNacreFluorescence microscopyMultidisciplinaryMicroscopy Confocallcsh:RfungiProteasesMolluscs[ SDV.IB.BIO ] Life Sciences [q-bio]/Bioengineering/BiomaterialsBivalviaMembraneAragonitechemistryBiophysicsUltrastructureMicroscopy Electron Scanninglcsh:QResearch ArticlePloS one
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An anti-inflammatory ditriazine inhibiting leukocyte functions and expression of inducible nitric oxide synthase and cyclo-oxygenase-2.

2000

A ditriazine derivative (4,10-dichloropyrido[5,6:4,5]thieno[3,2-d':3, 2-d]-1,2,3-ditriazine (DTD)) inhibited neutrophil functions, including degranulation, superoxide generation, and leukotriene B(4) production, without any effect on 5-lipoxygenase activity. This compound reduced nitric oxide (NO) and prostaglandin E(2) production in mouse peritoneal macrophages stimulated with lipopolysaccharide, whereas no influence on the activity of inducible NO synthase, cyclo-oxygenase-2 or cyclo-oxygenase-1 was observed. DTD significantly reduced mouse paw oedema induced by carrageenan and also markedly reduced NO and prostaglandin E(2) levels in exudates from 24-h zymosan-stimulated mouse air pouch.…

LipopolysaccharideLeukotriene B4Neutrophilsmedicine.medical_treatmentAnti-Inflammatory AgentsNitric Oxide Synthase Type IICarrageenanNeutrophil Activationchemistry.chemical_compoundMiceLeukocytesEdemaProstaglandin E2biologyPancreatic ElastaseSuperoxideTriazinesHindlimbNitric oxide synthaseIsoenzymesBiochemistryFemalemedicine.drugProstaglandin EBlood PlateletsLeukotriene B4DinoprostonePhospholipases ANitric oxideMicrosomesmedicineAnimalsHumansNitritesPharmacologyInflammationCell-Free SystemDose-Response Relationship DrugZymosanMembrane ProteinsMolecular biologyThromboxane B2chemistryEicosanoidCyclooxygenase 2Prostaglandin-Endoperoxide SynthasesLuminescent Measurementsbiology.proteinMacrophages PeritonealNitric Oxide SynthaseEuropean journal of pharmacology
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A new chloroquinolinyl chalcone derivative as inhibitor of inflammatory and immune response in mice and rats

2003

AbstractThe synthetic chalcone derivative 1-(2,4-dichlorophenyl)-3-(3-(6,7-dimethoxy-2-chloroquinolinyl))-2-propen-1-one (CIDQ) was evaluated for its anti-inflammatory, analgesic and immunomodulatory efficacy in-vitro and in-vivo. CIDQ concentration-dependently inhibited the production of nitric oxide (NO) (IC50 4.3 μM) and prostaglandin E2 (PGE2) (IC50 1.8 μM) in RAW 264.7 macrophages stimulated with lipopolysaccharide. Human mononuclear cell proliferation was significantly inhibited by 10 μM CIDQ. Oral administration of CIDQ (10–30 mg kg−1) in the 24-h zymosan-stimulated mouse air-pouch model produced a dose-dependent reduction of cell migration as well as NO and PGE2 levels in exudates. …

LipopolysaccharidesLipopolysaccharidemedicine.medical_treatmentAdministration OralPharmaceutical ScienceAbdominal InjuriesPharmacologyLymphocyte ActivationMicechemistry.chemical_compoundProstaglandin E2Pain MeasurementPyridazinesCytokineFemalemedicine.symptomProstaglandin Emedicine.drugBlood PlateletsChalconeMononuclear cell proliferationPainInflammationGroup II Phospholipases A2DinoprostonePhospholipases ACell LineNitric oxideDrug HypersensitivityFormaldehydeMicrosomesmedicineAnimalsHumansNitritesInflammationPharmacologyDose-Response Relationship Drugbusiness.industryGroup IV Phospholipases A2MacrophagesZymosanArthritis ExperimentalRatsThromboxane B2Disease Models AnimalchemistryCyclooxygenase 2Rats Inbred LewImmunologyCyclooxygenase 1DinitrofluorobenzenebusinessJournal of Pharmacy and Pharmacology
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Platelet count does not predict bleeding in cirrhotic patients: Results from the PRO-LIVER Study

2018

OBJECTIVES: Thrombocytopenia is a hallmark for patients with cirrhosis and it is perceived as a risk factor for bleeding events. However, the relationship between platelet count and bleeding is still unclear. METHODS: We investigated the relationship between platelet count and major or clinical relevant nonmajor bleedings during a follow-up of ∼4 years. RESULTS: A total of 280 cirrhotic patients with different degrees of liver disease (67% males; age 64±37 years; 47% Child–Pugh B and C) were followed up for a median of 1,129 (interquartile range: 800–1,498) days yielding 953.12 patient-year of observation. The annual rate of any significant bleeding was 5.45%/year (3.57%/year and 1.8…

Liver CirrhosisMaleSettore MED/09 - Medicina Interna030204 cardiovascular system & hematologyGastroenterologySeverity of Illness IndexcjirrhosisACTIVATION0302 clinical medicineRisk FactorsMedicinePlateletProspective StudiesProspective cohort studyRISKAged 80 and overmedicine.diagnostic_testPRO-LIVERPlatelet cirrhosis gastrointestinal bleedingPlateletGastroenterologyASSOCIATIONMiddle AgedPrognosisItaly030211 gastroenterology & hepatologyFemaleGastrointestinal HemorrhageHumanAdultPlateletsmedicine.medical_specialtyPrognosiLiver CirrhosiMEDLINECOAGULATIONgastrointestinal bleedingSocio-culturaleHemorrhageHepatology; GastroenterologyFollow-Up Studie03 medical and health sciencesText miningInternal medicineSeverity of illnessENDOTOXEMIAPro-Liver StudyHumansHEMOSTASISInternational Normalized RatioAgedProportional Hazards ModelsProthrombin timeCirrhosiHepatologyPlatelet Count Bleeding Liver Cirrhosisbusiness.industryProportional hazards modelPlatelet CountRisk FactorcirrhosisHepatologybleedingThrombocytopeniaProspective StudieTHROMBOSISPlatelets cjirrhosis bleeding PRO-LIVERProportional Hazards ModelProthrombin TimebusinessDECOMPENSATED CIRRHOSISFollow-Up Studies
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The Microbiota Promotes Arterial Thrombosis in Low-Density Lipoprotein Receptor-Deficient Mice

2019

Our results demonstrate a functional role for the commensal microbiota in atherothrombosis. In a ferric chloride injury model of the carotid artery, GF C57BL/6J mice had increased occlusion times compared to colonized controls. Interestingly, in late atherosclerosis, HFD-fed GF Ldlr−/− mice had reduced plaque rupture-induced thrombus growth in the carotid artery and diminished ex vivo thrombus formation under arterial flow conditions.

Male0209 industrial biotechnologyVery low-density lipoproteinChemokine CXCL102 engineering and technology030204 cardiovascular system & hematologyarterial thrombosisApplied Microbiology and BiotechnologyACTIVATIONMicechemistry.chemical_compound020901 industrial engineering & automation0302 clinical medicinegermfree0202 electrical engineering electronic engineering information engineeringMedicinevascular inflammationPlateletChemokine CCL7lcsh:QH301-705.5platelet0303 health sciencesatherosclerosis mouse modelsfood and beveragesThrombosisPlaque AtheroscleroticQR1-502late atherosclerosis3. Good healthHolobiontlow-density lipoprotein receptorgerm-freeplateletscardiovascular systemFemalelipids (amino acids peptides and proteins)GLYCOPROTEIN-VIBlood streamResearch ArticleRECRUITMENTmedicine.medical_specialtyNutritional compositionCOAGULATION610 Medicine & healthBiologyMETABOLISMBiochemistry Genetics and Molecular Biology (miscellaneous)MicrobiologyMicrobiologyHost-Microbe BiologyProinflammatory cytokinePLATELET HYPERREACTIVITY03 medical and health sciencesINFLAMMATIONVirologyInternal medicineatherothrombosisGeneticsmicrobiotaAnimalsInterleukin 9Platelet activationcardiovascular diseasesThrombusMolecular Biology030304 developmental biologygut microbiotabusiness.industryCholesterolcarotid artery020208 electrical & electronic engineeringcholesterolnutritional and metabolic diseasesCell Biologymedicine.diseaseMicroreviewCHLAMYDIA-PNEUMONIAEMice Mutant StrainsGastrointestinal MicrobiomeEndocrinologyReceptors LDLlcsh:Biology (General)chemistryArterial thrombusLDL receptorParasitologyatherosclerosisbusinessEx vivoLipoproteinmBio
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Comprehensive platelet phenotyping supports the role of platelets in the pathogenesis of acute venous thromboembolism - results from clinical observa…

2020

Background: The pathogenesis of arterial and venous thrombosis is in large part interlaced. How much platelet phenotype relates to acute venous thromboembolism (VTE) independent of the underlying cardiovascular profile is presently poorly investigated.Methods: Platelet count and mean platelet volume (MPV), platelet aggregation in whole blood and platelet rich plasma (PRP), platelet-dependent thrombin generation (TG) and platelet surface activation markers were measured under standardized conditions. Machine learning was applied to identify the most relevant characteristics associated with VTE from a large array (N = 58) of clinical and plateletrelated variables.Findings: VTE cases (N = 159)…

Male0301 basic medicinePlatelet Aggregationlcsh:MedicineDETERMINANTSGastroenterologyMachine LearningPathogenesisACTIVATION0302 clinical medicineRisk FactorsPlateletWhole bloodlcsh:R5-920AspirinOUTCOMESThrombinVenous ThromboembolismGeneral MedicineMiddle AgedThrombosisVenous thrombosis030220 oncology & carcinogenesisAcute DiseaseFemaleDisease Susceptibilitylcsh:Medicine (General)Research Papermedicine.drugBlood Plateletsmedicine.medical_specialtyPlatelet Function TestsGeneral Biochemistry Genetics and Molecular BiologyImmunophenotyping03 medical and health sciencesACUTE PULMONARY-EMBOLISMRISK-FACTORInternal medicinemedicineHumansMean platelet volumeMETAANALYSISAgedPlatelet Countbusiness.industrylcsh:RPlatelet Activationmedicine.diseasePREVENTIONASPIRINTHROMBOSIS030104 developmental biologyPlatelet-rich plasmaVOLUMEbusinessBiomarkersEBioMedicine
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