Search results for "Poly(acrylamide)"

showing 10 items of 377 documents

Characterization of the porcine seminal plasma proteome comparing ejaculate portions.

2016

Full identification of boar seminal plasma (SP) proteins remains challenging. This study aims to provide an extensive proteomic analysis of boar SP and to generate an accessible database of boar SP-proteome. A SP-pool (33 entire ejaculates/11 boars; 3 ejaculates/boar) was analyzed to characterize the boar SP-proteome. Twenty ejaculates (5 boars, 4 ejaculates/boar) collected in portions (P1: first 10 mL of sperm rich ejaculate fraction (SRF), P2: rest of SRF and P3: post-SRF) were analyzed to evaluate differentially expressed SP-proteins among portions. SP-samples were analyzed using a combination of SEC, 1-D SDS PAGE and NanoLC-ESI-MS/MS followed by functional bioinformatics. The identified…

0301 basic medicineMaleProteomicsendocrine systemBOARProteomeSwineQuantitative proteomicsBiophysicsComputational biologyBioinformatik och systembiologiBiologyBioinformaticsBiochemistry03 medical and health sciencesTandem Mass SpectrometryAnimalsPorcine; Ejaculate; Seminal plasma; ProteomeEjaculationSperm qualityDatabases ProteinLabel freeBioinformatics and Systems Biologyurogenital systemProteomic ProfilingReproductionSeminal Plasma ProteinsComputational BiologySpermSemen Analysis030104 developmental biologyFertilityGene Expression RegulationBiological significanceProteomeElectrophoresis Polyacrylamide GelChromatography LiquidJournal of proteomics
researchProduct

Functional display of an alpha2 integrin-specific motif (RKK) on the surface of baculovirus particles.

2005

The use of baculovirus vectors shows promise as a tool for gene delivery into mammalian cells. These insect viruses have been shown to transduce a variety of mammalian cell lines, and gene transfer has also been demonstrated in vivo. In this study, we generated two recombinant baculovirus vectors displaying an integrin-specific motif, RKK, as a part of two different loops of the green fluorescent protein (GFP) fused with the major envelope protein gp64 of Autographa californica M nucleopolyhedrovirus. By enzyme linked immunosorbent assays, these viruses were shown to bind a peptide representing the receptor binding site of an α2 integrin, the α2I-domain. However, the interaction was not st…

0301 basic medicineModels MolecularCancer ResearchInsectavirusesmedia_common.quotation_subjectAmino Acid MotifsGreen Fluorescent ProteinsIntegrin alpha2PeptideEnzyme-Linked Immunosorbent AssayCHO CellsBiologyGene deliveryGreen fluorescent proteinCell Line03 medical and health sciences0302 clinical medicineCricetinaeAnimalsCloning MolecularInternalizationmedia_commonchemistry.chemical_classificationMicroscopy ConfocalPhospholipase CWild typeGene Transfer Techniquesbiology.organism_classificationFlow CytometryMolecular biologyRecombinant ProteinsProtein Structure TertiaryAutographa californica030104 developmental biologyEnzymeOncologychemistryMicroscopy FluorescenceMutagenesis030220 oncology & carcinogenesisType C PhospholipasesElectrophoresis Polyacrylamide GelPeptidesBaculoviridaeViral Fusion ProteinsPlasmidsProtein BindingTechnology in cancer researchtreatment
researchProduct

Artefactual band patterns by SDS-PAGE of the Vip3Af protein in the presence of proteases mask the extremely high stability of this protein.

2018

Abstract Vip3 proteins are secretable proteins from Bacillus thuringiensis with important characteristics for the microbiological control of agricultural pests. The exact details of their mode of action are yet to be disclosed and the crystallographic structure is still unknown. Vip3 proteins are expressed as protoxins that have to be activated by the insect gut proteases. A previous study on the peptidase processing of Vip3Aa revealed that the protoxin produced artefactual band patterns by SDS-PAGE due to the differential stability of this protein and the peptidases to SDS and heating (Bel et al., 2017 Toxins 9:131). To determine whether this phenomenon also applies to other Vip3A proteins…

0301 basic medicineProteases030106 microbiologyBacillus thuringiensisSpodopteraSpodopteraCleavage (embryo)Biochemistry03 medical and health sciencesBacterial ProteinsStructural BiologyBacillus thuringiensismedicineAnimalsMode of actionMolecular BiologyPolyacrylamide gel electrophoresisbiologyChemistryProtein StabilityfungiMidgutGeneral Medicinebiology.organism_classificationTrypsin030104 developmental biologyBiochemistryInsect ProteinsElectrophoresis Polyacrylamide Gelmedicine.drugPeptide HydrolasesInternational journal of biological macromolecules
researchProduct

Protein denaturation caused by heat inactivation detrimentally affects biomolecular corona formation and cellular uptake

2018

Adsorption of blood proteins to the surface of nanocarriers is known to be the critical factor influencing cellular interactions and eventually determining the successful application of nanocarriers as drug carriers in vivo. There is an increasing number of reports summarizing large data sets of all identified corona proteins. However, to date our knowledge about the multiple mechanisms mediating interactions between proteins and nanocarriers is still limited. In this study, we investigate the influence of protein structure on the adsorption process and focus on the effect of heat inactivation of serum and plasma, which is a common cell culture procedure used to inactivate the complement sy…

0301 basic medicineProtein DenaturationHot TemperatureProtein Corona02 engineering and technologyMass SpectrometryMice03 medical and health sciencesProtein structureAdsorptionIn vivoAnimalsGeneral Materials ScienceChromatography High Pressure LiquidCalorimetry Differential ScanningChemistryBlood Proteins021001 nanoscience & nanotechnologyBlood proteinsProtein Structure TertiaryComplement systemClusterinRAW 264.7 Cells030104 developmental biologyBiophysicsNanoparticlesPolystyrenesElectrophoresis Polyacrylamide GelProtein CoronaNanocarriers0210 nano-technologyDrug carrier
researchProduct

Iron-loaded transferrin (Tf) is detrimental whereas iron-free Tf confers protection against brain ischemia by modifying blood Tf saturation and subse…

2018

Despite transferrin being the main circulating carrier of iron in body fluids, and iron overload conditions being known to worsen stroke outcome through reactive oxygen species (ROS)-induced damage, the contribution of blood transferrin saturation (TSAT) to stroke brain damage is unknown. The objective of this study was to obtain evidence on whether TSAT determines the impact of experimental ischemic stroke on brain damage and whether iron-free transferrin (apotransferrin, ATf)-induced reduction of TSAT is neuroprotective. We found that experimental ischemic stroke promoted an early extravasation of circulating iron-loaded transferrin (holotransferrin, HTf) to the ischemic brain parenchyma.…

0301 basic medicineU-PAGE urea-polyacrylamide gel electrophoresisMaleClinical BiochemistryExperimental strokeBiochemistryBrain IschemiaBrain ischemia0302 clinical medicineADC apparent diffusion coefficientApotransferrinDWI diffusion-weighted imagingTANDEM-1 Thrombolysis and Deferoxamine in Middle Cerebral Artery Occlusion clinical trialrHTf rat HTfrATf rat ATflcsh:QH301-705.5chemistry.chemical_classificationNeuronslcsh:R5-920ChemistryTransferrinExtravasationNS21 a medium supplement to grow neuronspDAPK-1 phosphorylated anti-death-associated protein kinase 1NeuroprotectionStrokeWB Western blotFemalemedicine.symptomlcsh:Medicine (General)Research PaperhHTf human HTfPC12 cell line derived from a pheochromocytoma of the rat adrenal medullamedicine.medical_specialtyIron OverloadBBB blood-brain barrierNMDAR N-methyl-D-aspartate receptorDCF dihydrofluoresceinIronWGA wheat germ agglutininHTf holotransferrinTransferrin receptorBrain damageTfR transferrin receptorDeferoxamineNeuroprotectionPI propidium iodide03 medical and health sciencesBrain damageCM conditioned mediumROS reactive oxygen speciesInternal medicine4-HNE 4-hydroxynonenalTf transferrinReceptors TransferrinmedicineFeRhoNoxTM-1 probe to detect Fe2+AnimalsHumansATf apotransferrinCM-H2DCFDA 5-chloromethyl-27-dichlorodihydrofluorescein diacetateMCAO middle cerebral artery occlusionDMT-1 divalent metal transporterB-27 a medium supplement to grow neuronsReactive oxygen speciesNMDA N-methyl-D-aspartateTSAT blood transferrin saturationTransferrin saturationBlood transferrin saturation (TSAT)Organic ChemistryNIR near infraredReactive oxygen species (ROS)medicine.diseasepMCAO permanent middle cerebral artery occlusionRatsPWI perfusion-weighted imaging030104 developmental biologyEndocrinologylcsh:Biology (General)TransferrinDAPK-1 anti-death-associated protein kinaseOGD oxygen/glucose deprivationTTC 235-triphenyl-tetrazolium chlorideLipid PeroxidationMCA middle cerebral arteryApoproteinsReactive Oxygen SpeciesMRI magnetic resonance imagingtMCAO transient middle cerebral artery occlusion030217 neurology & neurosurgeryhATf human ATf
researchProduct

Specific inflammatory response of Anemonia sulcata (Cnidaria) after bacterial injection causes tissue reaction and enzymatic activity alteration

2015

The evolution of multicellular organisms was marked by adaptations to protect against pathogens. The mechanisms for discriminating the ’’self’’ from ’’non-self” have evolved into a long history of cellular and molecular strategies, from damage repair to the co-evolution of host-pathogen interactions. We investigated the inflammatory response in Anemonia sulcata (Cnidaria: Anthozoa) following injection of substances that varied in type and dimension, and observed clear, strong and specific reactions, especially after injection of Escherichia coli and Vibrio alginolyticus. Moreover, we analyzed enzymatic activity of protease, phosphatase and esterase, showing how the injection of different ba…

0301 basic medicinemedicine.medical_treatmentPhosphatasemedicine.disease_causeEsteraseMicrobiology03 medical and health sciences0302 clinical medicineEscherichia colimedicineAnimals030212 general & internal medicineEscherichia coliInflammation Anemonia sulcata Cnidaria Bacterial injection Esterases PhosphatasesVibrio alginolyticusEcology Evolution Behavior and SystematicsInflammationchemistry.chemical_classificationVibrio alginolyticusProteasebiologyFibrinolysisEsterasesFibrinogenAlkaline Phosphatasebiology.organism_classificationPhosphoric Monoester HydrolasesSea Anemones030104 developmental biologyEnzymechemistryHost-Pathogen InteractionsGelatinAlkaline phosphataseElectrophoresis Polyacrylamide GelBacteriaDensitometryPeptide HydrolasesJournal of Invertebrate Pathology
researchProduct

Polyoxypregnanes as safe, potent, and specific ABCB1-inhibitory pro-drugs to overcome multidrug resistance in cancer chemotherapy in vitro and in vivo

2021

Multidrug resistance (MDR) mediated by ATP binding cassette subfamily B member 1 (ABCB1) is significantly hindering effective cancer chemotherapy. However, currently, no ABCB1-inhibitory drugs have been approved to treat MDR cancer clinically, mainly due to the inhibitor specificity, toxicity, and drug interactions. Here, we reported that three polyoxypregnanes (POPs) as the most abundant constituents of Marsdenia tenacissima (M. tenacissima) were novel ABCB1-modulatory pro-drugs, which underwent intestinal microbiota-mediated biotransformation in vivo to generate active metabolites. The metabolites at non-toxic concentrations restored chemosensitivity in ABCB1-overexpressing cancer cells v…

ABCC1 ATP binding cassette subfamily C member 1IC50 half maximal inhibitory concentrationMultidrug resistancePharmacologyNADPH reduced nicotinamide adenine dinucleotide phosphateF bioavailabilitychemistry.chemical_compoundPCR polymerase chain reaction0302 clinical medicineMDR multidrug resistanceECL electrochemiluminescencet1/2 elimination half-lifeLC–MS liquid chromatography coupled with mass spectrometryN.D. not detectedGeneral Pharmacology Toxicology and PharmaceuticsBBB blood–brain barriermedia_commonATF3 activating transcription factor 30303 health sciencesChemistryABC ATP-binding cassetteNMPA National Medical Products AdministrationPXR pregnane X receptorSDS-PAGE sodium dodecyl sulfate-polyacrylamide gel electrophoresisHBSS Hankʹs balanced salt solutionABCB1Combination chemotherapyProdrugMarsdenia tenacissimaCmax peak concentrationPaclitaxelGAPDH glyceraldehyde-3-phosphate dehydrogenase030220 oncology & carcinogenesisBHI brain heart infusionOriginal ArticleAUC0–∞ area under plasma concentration vs. time curveMRT mean residence timeDrugmedia_common.quotation_subjectRM1-950Vd volume of distributionABCB1 ATP binding cassette subfamily B member 1UIC-2 mouse monoclonal ABCB1 antibodyABCG2 ATP binding cassette subfamily G member 2Combination chemotherapyCYP cytochrome P450 isozymePI propidium iodideTEER transepithelial electrical resistance03 medical and health sciencesPBS phosphate buffer salineFBS fetal bovine serumDox doxorubicinIn vivoPOP polyoxypregnanemedicine030304 developmental biologyEVOM epithelial tissue voltohmmeterTmax time for peak concentrationCancerLBE lowest binding energyPE phycoerythrinmedicine.diseaseMultiple drug resistancePolyoxypregnanePapp apparent permeabilityN.A. not applicableCancer cellH&E hematoxylin and eosinMDR1a multidrug resistance protein 1aTherapeutics. PharmacologyqPCR quantitative PCRM. tenacissima Marsdenia tenacissimaCL clearanceSD standard derivationActa Pharmaceutica Sinica B
researchProduct

Effects of fatiguing jumping exercise on mRNA expression of titin-complex proteins and calpains.

2009

Eccentric exercise induced by electrostimulation increases mRNA expression of titin-complex proteins in rodent skeletal muscle. In this study, mRNA expression of titin, muscle LIM protein (MLP), cardiac ankyrin repeat protein (CARP), ankyrin repeat domain protein 2 (Ankrd2), diabetes-related ankyrin repeat protein (DARP), and calcium-activated proteinases, calpains, were investigated in human skeletal muscle after fatiguing jumping exercise. Fatiguing jumping exercise did not change mRNA expression of titin, DARP, calpain 1, or calpain 3. MLP, Ankrd2 and calpain 2 mRNA levels were increased 2 days postexercise. CARP mRNA level was already elevated 30 min and remained elevated 2 days postexe…

ANKRD2AdultMalemedicine.medical_specialtyPhysiologyMuscle ProteinsYoung AdultPhysiology (medical)Internal medicinemedicineMyocyteAnkyrinHumansConnectinRNA MessengerCarpMuscle SkeletalExercisechemistry.chemical_classificationbiologyCalpainSkeletal muscleCalpainbiology.organism_classificationAnkyrin RepeatBiomechanical Phenomenamedicine.anatomical_structureEndocrinologychemistryThighMuscle Fatiguebiology.proteinAnkyrin repeatTitinElectrophoresis Polyacrylamide Gelsense organsStress MechanicalProtein KinasesMuscle ContractionJournal of applied physiology (Bethesda, Md. : 1985)
researchProduct

Ag11(SG)7 : A New Cluster Identified by Mass Spectrometry and Optical Spectroscopy

2014

We report a one-step and high yield synthesis of a red-luminescent silver cluster with the molecular formula, Ag11(SG)7 (SG: glutathionate) via reduction of silver ions by sodium borohydride in the presence of the tripeptide, glutathione (GSH). The as-prepared cluster shows prominent absorption features at 485 and 625 nm in its UV-vis absorption spectrum. Aging of the as-prepared cluster solution led to the disappearance of the 625 nm peak, followed by broadening of the 485 nm peak to give three maxima at ?487, 437, and 393 nm in its absorption spectrum. These peaks remain unchanged even after polyacrylamide gel electrophoresis (PAGE), where a single band was observed confirming high purity…

Absorption spectroscopyta114Electrospray ionizationAnalytical chemistryMass spectrometryAbsorption spectra; Density functional theory; Electrodeposition; Electromagnetic wave absorption; Electrophoresis; Electrospray ionization; Isotopes; Light absorption; Mass spectrometry; Metal ions; Potable water; Spectrometry; Ultraviolet spectroscopy; Absorption features; Electrospray ionization mass spectrometry; High yield synthesis; Molecular formula; Optical spectroscopy; Polyacrylamide gel electrophoresis; Sodium boro hydrides; UV-VIS absorption spectra; Absorption spectroscopySurfaces Coatings and FilmsElectronic Optical and Magnetic MaterialsIonSodium borohydridechemistry.chemical_compoundGeneral EnergyUltraviolet visible spectroscopychemistryPhysical and Theoretical ChemistryAbsorption (electromagnetic radiation)Spectroscopyta116Journal of Physical Chemistry C
researchProduct

Glycoprotein and ganglioside changes in human trophoblasts after exposure to pulsed doppler ultrasound

1995

Changes in glycoprotein and ganglioside composition in human trophoblasts (eighth week of gestation) after in vitro exposure to pulsed Doppler ultrasound (pulse duration 1.22 microseconds; repetition frequency 11.1 kHz; center frequency 4 MHz; ISPPA = 175.5 W/cm2; ISPTA = 0.59 W/cm2) were investigated. Evacuated trophoblasts were divided in two halves and insonated for 10 min on top of a 6-cm layer of 5% gelatin in 50-mL tubes (Falcon) at 37 degrees C. One half of each trophoblast was sham insonated and served as an internal control. After insonation trophoblasts were maintained at 37 degrees C for 24 h. Glycoproteins were detected using alpha-D-mannose specific lectins from Galanthus nival…

Acoustics and UltrasonicsImmunoblottingBiophysicsMannoseG(M2) Gangliosidechemistry.chemical_compoundGangliosidesLectinsmedicineG(M3) GangliosideHumansRadiology Nuclear Medicine and imagingGlycoproteinschemistry.chemical_classificationMembrane GlycoproteinsGangliosideGalanthusRadiological and Ultrasound TechnologybiologyChemistryTrophoblastLectinMolecular biologyN-Acetylneuraminic AcidTrophoblastsMolecular WeightMembrane glycoproteinsglycoproteins; gangliosides; lectins; pulsed doppler ultrasoundmedicine.anatomical_structureUltrasonography Doppler PulsedG(M2) GangliosideImmunologySialic Acidsbiology.proteinElectrophoresis Polyacrylamide GelChromatography Thin LayerPlant LectinsGlycoproteinMannoseN-Acetylneuraminic acid
researchProduct