Search results for "Potassium"

showing 10 items of 930 documents

Intracellular accumulation of l-Arg, kinetics of transport, and potassium leak conductance in oocytes from Xenopus laevis expressing hCAT-1, hCAT-2A,…

2004

AbstractCationic amino acid transporters play an important role in the intracellular supply of l-Arg and the generation of nitric oxide. Since the transport of l-Arg is voltage-dependent, we aimed at determining the intracellular l-Arg concentration and describing the transport of l-Arg in terms of Michaelis–Menten kinetics, taking into account membrane voltage. The human isoforms of the cationic amino acid transporters, hCAT-1, hCAT-2A, and hCAT-2B, were expressed in oocytes from Xenopus laevis and studied with the voltage clamp technique and in tracer experiments. We found that l-Arg was concentrated intracellularly by all hCAT isoforms and that influx and efflux, in the steady state of e…

Patch-Clamp TechniquesVoltage clampXenopusBiophysicsArginineBiochemistryMembrane PotentialsXenopus laevisVoltage dependencehCATAnimalsPatch clampCationic Amino Acid Transporter 2y+Cationic Amino Acid Transporter 1Membrane potentialbiologyChemistryBiological TransportTransporterCell Biologybiology.organism_classificationVmaxKMKineticsBiochemistryConductanceOocytesPotassiumBiophysicsAmino Acid Transport Systems BasicEffluxSteady state (chemistry)IntracellularBiochimica et Biophysica Acta (BBA) - Biomembranes
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Sequential acquisition of cacophony calcium currents, sodium channels and voltage-dependent potassium currents affects spike shape and dendrite growt…

2014

During metamorphosis the CNS undergoes profound changes to accommodate the switch from larval to adult behaviors. In Drosophila and other holometabolous insects, adult neurons differentiate either from respecified larval neurons, newly born neurons, or are born embryonically but remain developmentally arrested until differentiation during pupal life. This study addresses the latter in the identified Drosophila flight motoneuron 5. In situ patch-clamp recordings, intracellular dye fills and immunocytochemistry address the interplay between dendritic shape, excitability and ionic current development. During pupal life, changes in excitability and spike shape correspond to a stereotyped, progr…

Patch-Clamp Techniquesmedia_common.quotation_subjectAction Potentialschemistry.chemical_elementCell EnlargementBiologyCalciumSodium ChannelsArticleMembrane PotentialsAnimalsPatch clampMetamorphosisIon channelmedia_commonMotor NeuronsMembrane potentialMicroscopy ConfocalGeneral NeuroscienceSodium channelOptical ImagingfungiMetamorphosis BiologicalDendritesImmunohistochemistryPotassium channelCell biologyDrosophila melanogasterchemistryPotassium Channels Voltage-GatedPotassiumCalciumNeuroscienceIntracellularEuropean Journal of Neuroscience
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Regional cerebral blood flow and regional metabolism in cold induced oedema.

1973

24 hours following a cold induced oedema in cats rCBF was measured in the lesion area, the bluish stained cortex immediately adjacent to the lesion, a cortical area remote from the lesion, and in the contralateral uninjured hemisphere. Thereafter the brain was frozen and the respective tissue areas were removed and analyzed for water and electrolyte content as well as metabolite concentrations. It seems, that in the neighbourhood of a local lesion at least 3 different brain regions can be differentiated with regard to their characteristic pattern of data. In non-oedematous regions either hyperaemia or hypoaemia could be observed. In areas with local brain oedema rCBF was reduced inversely p…

Pathologymedicine.medical_specialtyBrain EdemaPhosphocreatineMicrocirculationLesionHyperaemiachemistry.chemical_compoundAdenosine TriphosphateIschemiaCortex (anatomy)medicineAnimalsHypoxiaPyruvatesCerebrospinal FluidDiminutionCATSbusiness.industrySodiumBrainAnatomyWater-Electrolyte BalanceAdenosine MonophosphateAdenosine DiphosphateCold Temperaturemedicine.anatomical_structurechemistryCerebral blood flowRegional Blood FlowInjections IntravenousCatsLactatesPotassiumSurgeryNeurology (clinical)medicine.symptombusinessActa neurochirurgica
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18F-fluorodeoxyglucose hypometabolism in cerebellar tonsil and flocculus in downbeat nystagmus.

2006

A patient with downbeat nystagmus was examined by F-fluorodeoxyglucose-positron emission tomography once while off and twice while on successful treatment with 4-aminopyridine. All positron emission tomography scans of the patient showed a reduced cerebral glucose metabolism bilaterally in the region of the cerebellar tonsil and flocculus/paraflocculus when compared with a normal database of the whole brain. An additional region-of-interest analysis revealed that 4-aminopyridine treatment lessened the hypometabolism. This finding supports the hypothesis that the cerebellar tonsil and (para-) flocculus play a crucial role in downbeat nystagmus. The hypometabolism might reflect reduced inhibi…

Pathologymedicine.medical_specialtyCerebellumgenetic structuresEye MovementsNystagmusFlocculusNystagmus PathologicDownbeat nystagmusImaging Three-DimensionalVestibular nucleiFluorodeoxyglucose F18CerebellummedicinePotassium Channel BlockersHumans4-AminopyridineAgedFluorodeoxyglucosemedicine.diagnostic_testbusiness.industryGeneral Neurosciencemedicine.anatomical_structurePositron emission tomographyPositron-Emission TomographyCerebellar tonsilFemalemedicine.symptombusinessNeurosciencemedicine.drugNeuroreport
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Extracellular space and electrolyte distribution in cortex and white matter of dog brain in cold induced oedema

1973

24 hours after a circumscribed cold injury of the cortex dog brains were perfused from the lateral ventricle and the frontal subarachnoidal space to the cisterna magna with an artificial CSF containing trace amounts of35S-labelled thiosulphate. Simultaneously the extracellular tracer was administered intravenously. Extracellular fluid volume was estimated and found to be increased from 10 to 15% in the oedematous cortex and from 10 to 27% in the oedematous white matter. The actual size of ECS in oedematous white matter, however, must be larger as indicated by the relative alterations of thiosulphate distribution, tissue water, sodium and chloride. Apparently a small part of the fluid accumu…

Pathologymedicine.medical_specialtySodiumThiosulfateschemistry.chemical_elementBrain EdemaCisterna magnaIodine RadioisotopesWhite matterchemistry.chemical_compoundDogsChloridesCortex (anatomy)Sulfur IsotopesExtracellular fluidExtracellularAnimalsMedicineSerum Albumin Radio-IodinatedEvans BlueCerebral CortexStaining and Labelingbusiness.industrySodiumWater-Electrolyte BalanceCold TemperatureDisease Models Animalmedicine.anatomical_structurechemistryBlood-Brain BarrierCerebral cortexPotassiumSurgeryNeurology (clinical)Extracellular SpacebusinessActa Neurochirurgica
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Long-term follow-up and novel genotype-phenotype analysis of monozygotic twins with ATP1A3 mutation in Alternating Hemiplegia of Childhood-2

2020

Alternating Hemiplegia of Childhood (AHC) is a rare disorder characterized by frequent, transient attacks of hemiplegia involving either side of the body or both in association to several other disturbances including dystonic spells, abnormal ocular movements, autonomic manifestations, epileptic seizures and cognitive impairment. The clinical manifestations usually start before the age of 18 months. Two forms of the disorder known as AHC-1 (MIM#104290) and AHC-2 (MIM#614820) depends on mutations in ATP1A2 and ATP1A3 genes respectively, with over 75% of AHC caused by a mutation in the ATP1A3 gene. Herewith, we report serial clinical follow-up data of monozygotic (MZ) twin sisters, who presen…

Pediatricsmedicine.medical_specialtyGenotype-phenotype correlationGenotypeTwinsHemiplegiaMonozygoticEpilepsyYoung AdultSettore MED/38 - Pediatria Generale E SpecialisticaATP1A2Alternating Hemiplegia of Childhood (AHC)ATP1A3GenotypeGeneticsmedicineHumansYoung adultATPase Na+/K+ transporting subunit alpha 2 (ATP1A2)Genetics (clinical)DystoniaATPase Na+/K+ transporting subunit alpha 3 (ATP1A3)business.industryAlternating hemiplegia of childhoodp.Asn773SerGeneral MedicineTwins Monozygoticmedicine.diseasePhenotypePhenotypeMutationFemaleSodium-Potassium-Exchanging ATPasebusiness
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CCDC 619252: Experimental Crystal Structure Determination

2007

Related Article: L.Lisnard, P.Mialane, A.Dolbecq, J.Marrot, J.M.Clemente-Juan, E.Coronado, B.Keita, P.de Oliveira, L.Nadjo, F.Secheresse|2007|Chem.-Eur.J.|13|3525|doi:10.1002/chem.200601252

Penta-potassium tri-sodium (mu12-silicato)-bis(mu3-azido)-(mu3-hydroxo)-tris(mu2-acetato)-henicosakis(mu2-oxo)-nonaoxo-tetra-cobalt(ii)-nona-tungsten octadecahydrateSpace GroupCrystallographyCrystal SystemCrystal StructureCell ParametersExperimental 3D Coordinates
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Pressure dependence of the low-frequency dielectric constant of KNbO_3

1997

The effect of pressure on the low-frequency dielectric constant, $\epsilon_0$, of single crystals of KNbO_3 is investigated by means of capacitance measurements. The dielectric constant increases with pressure up to 22.5 kbar, where it exhibits a large value ($\epsilon_0$ = 5000), and then decreases. This change in its behaviour is related to a phase transition induced by pressure. On decompression, the samples do not revert back to the ambient pressure phase.

PermittivityCondensed Matter - Materials SciencePotassium niobateMaterials scienceCondensed matter physicsMaterials Science (cond-mat.mtrl-sci)FOS: Physical sciencesFrequency dependenceDielectricLow frequencyPressure dependenceCondensed Matter PhysicsCapacitanceElectronic Optical and Magnetic Materialschemistry.chemical_compoundchemistry
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Semi-empirical indo and shell-model calculations for perovskites

1999

Abstract Structural, phonon and some elastic and dielectric properties have been calculated for various paraelectric or ferroelectric phases of the perovskites KNbO3 and SrTiO3, using either the semi-empirical INDO (Intermediate Neglect of Differential Overlap) method or a temperature-dependent shell model. The INDO method was used to calculate the energy changes resulting from [100], [110] or [111] displacements of Nb atoms in the cubic perovskite cell of KNbO3, at 0K. The conventional shell model gives a good account of the elastic, dielectric and phonon properties of the cubic phase of strontium titanate at room temperature, but difficulties remain in modelling the permittivity and elast…

PermittivityNuclear and High Energy PhysicsRadiationPotassium niobateMaterials scienceCondensed matter physicsPhononMineralogyDielectricCondensed Matter PhysicsFerroelectricityCondensed Matter::Materials Sciencechemistry.chemical_compoundchemistryPhase (matter)Physics::Atomic and Molecular ClustersStrontium titanateGeneral Materials SciencePerovskite (structure)Radiation Effects and Defects in Solids
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Dual effect mediated by protease-activated receptors on the mechanical activity of rat colon

2002

1. The present study examined the mechanical effects of agonist enzymes and receptor-activating peptides for protease-activated receptor (PAR)-1 and PAR-2 on longitudinal and circular muscle of rat isolated colonic segments in the attempt to clarify the PAR functional role in intestinal motility. 2. The responses to PAR-1 and PAR-2 activation were examined in vitro by recording simultaneously the changes of endoluminal pressure (index of circular muscle activity) and of isometric tension (index of longitudinal muscle activity). 3. Both PAR-1 agonists, thrombin (0.1 nM - 3 microM) and SFLLRN-NH2 (1 nM - 3 microM), and PAR-2 agonists, trypsin (0.1 nM - 10 microM) and SLIGRL-NH2 (1 nM - 10 mic…

PharmacologyAgonistmedicine.medical_specialtymedicine.drug_classMotilityBiologyApaminPotassium channelchemistry.chemical_compoundEndocrinologychemistryInternal medicineTetrodotoxinmedicineProtease-activated receptormedicine.symptomReceptorMuscle contractionBritish Journal of Pharmacology
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