Search results for "Primer"
showing 10 items of 530 documents
Predictive value of the Merrill-Palmer-R Scale applied during the first year of live
2016
Resumen basado en el de la publicación Títulos, resumen y palabras clave en inglés y español El valor predictivo de una escala de desarrollo utilizado durante el primer año de vida es de gran interés en la planificación de las intervenciones tempranas. El valor predictivo de un instrumento es la probabilidad de acertar el diagnóstico de trastorno o retraso en el desarrollo de un niño. Se analizaron las puntuaciones de escala Merrill-Palmer-R (MP-R) en una muestra de 291 niños menores de un año de la Comunidad Valenciana. A pesar de que no se distribuyen normalmente las puntuaciones de la escala MP-R en esta muestra, los resultados predichos eran buenos. Además, las puntuaciones de desarroll…
Educación y equidad en la primera infancia : el caso de las aulas de 2 años en los centros públicos de la Comunidad Valenciana
2022
En este trabajo analizamos la evolución de la provisión de educación y atención de la primera infancia (EAPI) en el primer ciclo de la Educación Infantil en el contexto de la Comunidad Valenciana, con particular atención al caso de la implantación de las aulas de 2 años en centros públicos. Durante las últimas dos legislaturas, la Comunidad Valenciana ha pasado de estar en la vanguardia del fomento y desarrollo del modelo de oferta privada a ser de las regiones pioneras en el desarrollo de las aulas de 2 años en la enseñanza pública. Enmarcamos estos cambios en el contexto español, y discutimos su alcance para la equidad del sistema educativo en esta etapa desde el enfoque de los derechos h…
Mitochondrial DNA variation and the evolutionary history of cryptic Gammarus fossarum types.
2000
The evolutionary history of the cryptic Gammarus fossarum species complex (Crustacea, Amphipoda) in Central Europe was approached by investigating the genetic variation in populations of a natural contact zone. Nucleotide sequence variation of a 395-bp segment of the mitochondrial 16S rRNA gene was compared to that of six nuclear allozyme loci. Three major mtDNA lineages were found, the eastern clade being consistent with the former allozyme type A. The two western clades (types B and C) were not distinguished previously. Strong sequence divergence and correlation with nuclear genetic isolation in syntopic populations, however, justifies the specific status of the three G. fossarum types. T…
The age and evolution of sociality in Stegodyphus spiders: a molecular phylogenetic perspective
2006
Social, cooperative breeding behaviour is rare in spiders and generally characterized by inbreeding, skewed sex ratios and high rates of colony turnover, processes that when combined may reduce genetic variation and lower individual fitness quickly. On these grounds, social spider species have been suggested to be unstable in evolutionary time, and hence sociality a rare phenomenon in spiders. Based on a partial molecular phylogeny of the genus Stegodyphus , we address the hypothesis that social spiders in this genus are evolutionary transient. We estimate the age of the three social species, test whether they represent an ancestral or derived state and assess diversification relative to s…
Conciencia fonológica y resolución de problemas matemáticos en educación infantil
2018
Resumen Introduccion Algunos estudios revelan la participacion del procesamiento linguistico en la resolucion de problemas matematicos, subrayando su papel en la representacion y en el procesamiento de informacion. Estas investigaciones no suelen considerar poblacion en edades tempranas. Objetivos Este estudio tiene un doble objetivo: analizar la influencia linguistica en resolucion de problemas matematicos en la etapa de educacion infantil y analizar que habilidades linguisticas explican significativamente por si solas la resolucion de problemas matematicos. Metodo Participaron 76 ninos espanoles que cursaban tercero de educacion infantil. Se evaluaron habilidades semanticas, morfosintacti…
La utilización de marfil de cachalote en el Calcolítico de Portugal
2013
Scientific analysis were undertaken within a research project concerning ivory objects from the Chalcolithic to Early Bronze Age of the Iberian Peninsula. In several of the analyses of objects from Portuguese Estremadura, especially V-perforated buttons, we could detect for the first time the presence of sperm whale ivory. This highlights the advantage and necessity of scientific analysis of ivory. It also clearly demonstrates that not all ivory used was ivory from African or Asian elephants, but we also did find ivory from the extinct Elephas antiquus, the hippopotamus and in this case sperm-whale. Thus, already in the Chalcolithic the raw material provenience was highly diverse, which in …
SeqEditor: an application for primer design and sequence analysis with or without GTF/GFF files
2021
[Motivation]: Sequence analyses oriented to investigate specific features, patterns and functions of protein and DNA/RNA sequences usually require tools based on graphic interfaces whose main characteristic is their intuitiveness and interactivity with the user’s expertise, especially when curation or primer design tasks are required. However, interface-based tools usually pose certain computational limitations when managing large sequences or complex datasets, such as genome and transcriptome assemblies. Having these requirments in mind we have developed SeqEditor an interactive software tool for nucleotide and protein sequences’ analysis.
Production of Hev b5 as a fluorescent biotin-binding tripartite fusion protein in insect cells
2005
The presented green fluorescent protein and streptavidin core-based tripartite fusion system provides a simple and efficient way for the production of proteins fused to it in insect cells. This fusion protein forms a unique tag, which serves as a multipurpose device enabling easy optimization of production, one-step purification via streptavidin-biotin interaction, and visualization of the fusion protein during downstream processing and in applications. In the present study, we demonstrate the successful production, purification, and detection of a natural rubber latex allergen Hev b5 with this system. We also describe the production of another NRL allergen with the system, Hev b1, which fo…
Expression of solute carrier 7A4 (SLC7A4) in the plasma membrane is not sufficient to mediate amino acid transport activity.
2002
Member 4 of human solute carrier family 7 (SLC7A4) exhibits significant sequence homology with the SLC7 subfamily of human cationic amino acid transporters (hCATs) [Sperandeo, Borsani, Incerti, Zollo, Rossi, Zuffardi, Castaldo, Taglialatela, Andria and Sebastio (1998) Genomics 49, 230–236]. It is therefore often referred to as hCAT-4 even though no convincing transport activity has been shown for this protein. We expressed SLC7A4 in Xenopus laevis oocytes, but could not detect any transport activity for cationic, neutral or anionic amino acids or for the polyamine putrescine. In addition, human glioblastoma cells stably overexpressing a fusion protein between SLC7A4 and the enhanced green f…
Bradykinin-induced Internalization of the Human B2Receptor Requires Phosphorylation of Three Serine and Two Threonine Residues at Its Carboxyl Tail
1999
The binding of bradykinin (BK) to B2 receptor triggers the internalization of the agonist-receptor complex. To investigate the mechanisms and the receptor structures involved in this fundamental process of receptor regulation, the human B2 receptor was mutated within its cytoplasmic tail by complementary strategies of truncation, deletion, and amino acid substitution. Ligand binding, signal transduction, internalization as well as phosphorylation were studied for the mutated receptors expressed in COS, CHO, and HEK 293 cells. Truncation of 44 out of 55 amino acid residues of the receptor's cytoplasmic tail corresponding to positions 321-364 did not alter the kinetics of BK binding and the r…