Search results for "Prom"
showing 10 items of 2286 documents
Cloning and characterization of the promoter of Hugl-2, the human homologue of Drosophila lethal giant larvae (lgl) polarity gene.
2007
The human lgl gene, Hugl-2 (llgl2, Lgl2), codes for a cytoskeletal protein involved in regulating cell polarity. Here, we report the identification and functional characterization of the promoter region ( approximately 1.2kb) of the Hugl-2 gene. Luciferase expression assays show a high basal Hugl-2 promoter activity in different cell lines and primary human hepatocytes. Truncations of the promoter identified a GC-rich region important for this activity. Alignment of human and mouse genomic sequences demonstrate that this is an evolutionary conserved region fcontaining putative binding sites for several transcription factors including Elk-1 and Sp-1. Mithramycin A reduces Hugl-2 expression i…
STANDARD OPERATING PROCEDURES IN SPORTS SCIENCES AND HEALTH PROMOTION PROGRAMS IN DIFFERENT POPULATIONS
2021
In recent years, the population is becoming more sedentary and characterized by an increase in the mean age. One of the consequences is a population with a higher risk of chronic diseases, increasing the risk to incur in disability and death. Consequently, health promotion programs, cheaper than interventions, are acquiring an increasingly central role in the politics of Governments. Health promotion programs want to maintain and improve the physical, cognitive, psychical, and social sphere of people. Considering that psychic and social sphere are strictly dependent on the physical condition, the interest of this thesis was on motor tests ideal for health promotion programs. The motor evalu…
La elección del momento oportuno de la política ambiental en un mercado duopolístico
2015
[EN] In this paper the strategic use of innovation by two polluting firms to influence environ-mental policy is evaluated. The analysis is carried out by comparing two alternative policy regimes for two policy instruments: Taxes and standards. The first of the regimes assumes that the regulator commits to an ex-ante level of the policy instrument. In the second one, there is no commitment. The results show that when there is no commitment and a tax is used to control emissions, the strategic behavior of firms can be welfare improving if the efficiency of the clean technology is relatively low. If this is not the case, the strategic behavior of the duopolists has a detrimental effect on welf…
Subcytocidal attack by staphylococcal alpha-toxin activates NF-kappaB and induces interleukin-8 production.
2001
ABSTRACTFormation of transmembrane pores by staphylococcal alpha-toxin can provoke a spectrum of events depending on target cell species and toxin dose, and in certain cases, repair of the lesions has been observed. Here, we report that transcriptional processes are activated as a response of cells to low toxin doses. Exposure of monocytic (THP-1) or epithelial (ECV304) cells to 40 to 160 ng/ml alpha-toxin provoked a drop in cellular ATP level that was followed by secretion of substantial amounts of interleukin-8 (IL-8). Cells transfected with constructs comprising the proximal IL-8 promoter fused to luciferase or to green fluorescent protein cDNA exhibited enhanced reporter gene expression…
Comparing the Antileishmanial Activity of Gold(I) and Gold(III) Compounds in L. amazonensis and L. braziliensis in Vitro
2020
Abstract Abstract: A series of mononuclear coordination or organometallic AuI/AuIII complexes (1–9) have been comparatively studied in vitro for their antileishmanial activity against promastigotes and amastigotes, the clinically relevant parasite form, of Leishmania amazonensis and Leishmania braziliensis. One of the cationic AuI bis‐N‐heterocyclic carbenes (3) has low EC50 values (ca. 4 μM) in promastigotes cells and no toxicity in host macrophages. Together with two other AuIII complexes (6 and 7), the compound is also extremely effective in intracellular amastigotes from L. amazonensis. Initial mechanistic studies include an evaluation of the gold complexes′ effect on L. amazonensis’ pl…
Identification of a Terphenyl Derivative that Blocks the Cell Cycle in the G0−G1 Phase and Induces Differentiation in Leukemia Cells
2006
To further explore the SAR of resveratrol-related trans-stilbene derivatives, here we describe the synthesis of (a) a series of 3,5-dimethoxy analogues in which a variety of substituents were introduced at positions 2', 3', 4', and 5' of the stilbene scaffold and (b) a second group of derivatives (2-phenylnaphthalenes and terphenyls) that incorporate a phenyl ring as a bioisosteric replacement of the stilbene alkenyl bridge. We thoroughly characterized all of the new compounds with respect to their apoptosis-inducing activity and their effects on the cell cycle. One of the new derivatives, 13g, behaved differently from the others, as it was able to block the cell cycle in the G(0)-G(1) phas…
Membrane D-lactate oxidase in Zymomonas mobilis: evidence for a branched respiratory chain.
1998
Respiratory chain composition of the ethanol-producing bacterium Zymomonas mobilis was studied. Its membrane D-lactate oxidase was characterised. With NADH, but not D-lactate as substrate, a cytochrome o-like component was seen in CO difference spectra. Chlorpromazine specifically inhibited reduction of cytochrome d, while myxothiazol eliminated the cytochrome o-like features in CO difference spectra. It is suggested that electrons from NADH are distributed between branches terminated by the cytochrome o-like component, cytochrome a, and cytochrome d. With D-lactate, electrons are transported to cytochrome a, or an unidentified CN(-)-sensitive oxidase, and cytochrome d.
Overcoming the “lost before translation” problem: An exploratory study
2019
This paper draws on Stokes’ (1997) framework to position the disconnection between theory and practice as a knowledge production problem. In this sense, we argue that a better understanding of different academic profiles is extremely important to focus efforts on those academics that may overcome the ‘lost before translation’ problem. Our data, that come from a survey of researchers affiliated to the Spanish National Research Council (CSIC), provide a good opportunity to explore the factors that might increase or impede the likelihood that researchers engage in research that reconciles the quest for fundamental understanding with the consideration of use (Pasteur’s profile), rather than in …
The Role of Electronic Channels in Micro-Sized Brick-and-Mortar Firms
2010
The purpose of this study is to increase understanding of the role of electronic channels in micro-sized brick-and-mortar firms, which are just starting to utilize new channels in the promotion of their goods and services. With the use of a multiple case study method containing ten cases, the study first identifies external and internal factors affecting the adoption and use of e-channels in micro-sized firms. Secondly, the results propose, in line with previous literature, that different e-channel utilization levels can be identified. The paper provides both theoretical and managerial implications. peerReviewed
REGULATORY ELEMENTS OF THE LEUKAEMIA INHIBITORY FACTOR (LIF) PROMOTER IN MURINE BONE MARROW STROMAL CELLS
1999
Leukaemia inhibitory factor (LIF) plays an important role as a haematopoietically active cytokine. As described earlier in a murine model, interleukin 1 (IL-1) induced LIF mRNA and protein expression. We utilized the murine cell line +/+-1.LDA11 to further define regulatory mechanisms of LIF expression in bone marrow stromal cells. The production of LIF mRNA is stimulated by IL-1beta, TNF-alpha, and the cAMP analogue 8-bromoadenosine 3':5'-monophosphate (8BrcAMP). LIF mRNA expression is controlled at the transcriptional level. Different fragments from -542 to -45 bp 5' upstream of the transcriptional start site of the murine LIF gene were fused to the luciferase gene. All LIF-promoter lucif…