Search results for "Protocol"
showing 10 items of 1808 documents
Design of antitumor drugs targeting c-kit receptor by a new mixed ligand-structure based method
2020
An important challenge, in the medicinal chemistry field, is the research of novel forceful drugs to overcome tumor-acquired resistance. The c-Kit tyrosine kinase receptor (TKR) represents a suitable target for the carcinogenesis control of gastro-intestinal stromal (GIST), leukemia, and mastocytosis tumors; nevertheless, several hotspot mutations of the protein limit the efficacy of a few clinical administered TKRs inhibitors. In this study, a new in silico protocol based on ligand and structure-based combined method is proposed, with the aim to identify a set of new c-Kit inhibitors able to complex c-Kit mutated proteins. A recent and freely available web-server DRUDIT is used for the lig…
A Language and Platform Independent Co-Simulation Framework Based on the Functional Mock-Up Interface
2019
The main goal of the Functional Mock-up Interface (FMI) standard is to allow the sharing of simulation models across tools. To accomplish this, FMI relies on a combination of XML-files and compiled C-code packaged in a zip archive. This archive is called a Functional Mock-up Unit (FMU). In theory, an FMU can support multiple platforms, but not necessarily in practice. Furthermore, software libraries for interacting with FMUs may not be available in a particular language or platform. Another issue is related to the protection of intellectual property (IP). While an FMU is free to only provide the C-code in its binary form, other resources within the FMU may be unprotected. Distributing model…
Model-based specification and validation of the dual-mode adaptive MAC protocol
2018
Wireless sensor and actuator networks (WSANs) rely on MAC protocols to coordinate access to the wireless medium access and for managing the radio unit on each device. The dual-mode adaptive MAC (DMAMAC) protocol is a recently proposed protocol designed to reduce the energy consumption of the radio communication in WSANs. The DMAMAC protocol targets the industrial WSANs used for real-time process control. At its core, DMAMAC exploits the distinction between transient and steady of the controlled plant process to dynamically adapt the MAC superframe structure and thereby conserve energy. The switch between steady and transient mode of operation is a safety-critical part of the protocol. The c…
GDL90fuzz: Fuzzing - GDL-90 Data Interface Specification Within Aviation Software and Avionics Devices–A Cybersecurity Pentesting Perspective
2022
As the core part of next-generation air transportation systems, the Automatic Dependent Surveillance-Broadcast (ADS-B) is becoming very popular. However, many (if not most) ADS-B devices and implementations support and rely on Garmin’s GDL-90 protocol for data exchange and encapsulation. In this paper, we research GDL-90 protocol fuzzing options and demonstrate practical Denial-of-Service (DoS) attacks on popular Electronic Flight Bag (EFB) software operating on mobile devices. For this purpose, we specifically configured our own avionics pentesting platform. and targeted the popular Garmin’s GDL-90 protocol as the industry-leading devices operate on it. We captured legitimate traffic from …
Homemade Site Directed Mutagenesis of Whole Plasmids
2009
Site directed mutagenesis of whole plasmids is a simple way to create slightly different variations of an original plasmid. With this method the cloned target gene can be altered by substitution, deletion or insertion of a few bases directly into a plasmid. It works by simply amplifying the whole plasmid, in a non PCR-based thermocycling reaction. During the reaction mutagenic primers, carrying the desired mutation, are integrated into the newly synthesized plasmid. In this video tutorial we demonstrate an easy and cost effective way to introduce base substitutions into a plasmid. The protocol works with standard reagents and is independent from commercial kits, which often are very expensi…
A report of an international collaborative experiment to demonstrate the uniformity obtainable using DNA profiling techniques
1992
This paper describes a collaborative exercise intended to demonstrate whether uniformity of DNA profile results could be achieved between different European laboratories. It was shown that this goal can be obtained provided that a common protocol is followed (specifically the use of a common electrophoretic buffer as being the most important parameter). Generally, lower molecular weight loci (with lower molecular weight fragments) such as YNH24 perform better than higher molecular weight loci such as MS43a. The results of the exercise are discussed in relation to the objectives of the European DNA profiling group (EDNAP).
A quick one-tube nested PCR-protocol for EPO transgene detection
2012
The practice of doping threatens fair competition in sports. With the very recent reports on successful gene therapies for several diseases, the likelihood for abuse of gene transfer techniques in elite sports is rapidly increasing. It is therefore very important to develop valid detection techniques for transgenic DNA (tDNA) with ultimate sensitivity and specificity. To date, three slightly different procedures have been reported to reliably detect tDNA with sufficiently high sensitivity. Two utilize a real-time PCR-based approach and one uses a primer-internal, intron-spanning PCR approach (spiPCR). The specificity and sensitivity of these techniques, however, is still a matter of debate.…
School Neighbourhood Built Environment Assessment for Adolescents’ Active Transport to School: Modification of an Environmental Audit Tool and Protoc…
2020
School neighbourhood built environments (SN-BE) can influence adolescents&rsquo
Prevalence and comorbidity of autism spectrum disorder in Spain: study protocol for a systematic review and meta-analysis of observational studies
2019
[Background] Autism spectrum disorder (ASD) is a complex developmental disorder characterised by impaired social interaction and communication, and restrictive and repetitive behaviour. Previous systematic reviews have traditionally assessed the prevalence of ASD on global or regional context, with very few meta-analyses at the country level. The objective of this study will be to systematically evaluate published and unpublished observational studies that present prevalence and comorbidity of ASD among children, adolescent and adult population in Spain.
Towards a physically more active lifestyle based on one's own values: study design of a randomized controlled trial for physically inactive adults
2013
Background This randomised controlled trial demonstrates the effectiveness of a value-based intervention program to encourage a physically more active lifestyle among physically inactive adults aged 30 to 50 years. The conceptual framework of the program is based on an innovative behavioural therapy called Acceptance and Commitment Therapy (ACT) that aims to increase an individual’s psychological flexibility and support behaviour change towards a higher quality and more meaningful life. Methods Participants will be randomly allocated to a feedback group (FB) or an Acceptance and Commitment based (ACT + FB) group. Both the groups will receive written feedback about their objectively measured…