Search results for "Purini"
showing 10 items of 29 documents
Nuclear expression of apurinic/apyrimidinic endonuclease increases with progression of ovarian carcinomas.
2003
Apurinic/apyrimidinic endonuclease (APE alias Ref-1) is a key enzyme in the base excision repair pathway. Besides its function in DNA repair, APE serves to maintain several transcription factors in an active reduced state such as c-Fos, c-Jun, NF-kappaB, p53 and HIF-1alpha, all of which have been shown to play a role in tumorigenesis. Because of the importance of APE in maintaining genomic stability and gene regulation, we examined whether APE expression is associated with survival and histopathological parameters of patients with ovarian cancer.Tissue sections of primary epithelial ovarian carcinomas from 141 patients were immunostained using a monoclonal antibody directed against APE.Nucl…
Purincyborg
2021
L’occhio cyborg di Franco Purini elabora le informazioni del reale per alimentare una personale ricerca sulla composizione della forma nelle pagine dei suoi taccuini da viaggio. Accumulando dati, ipotesi e prospettive, esso misura proiezioni visionarie in disegni e opere costruite tra loro interrelati e conseguenziali. Dando prova delle potenzialità del reale PURINCYBORG sintetizza i confini del pensiero umano traslandoli ben oltre ogni possibile traguardo della tecnologia.
Franco Purini Una lezione sul disegno
2015
Recensione al volume "Franco Purini Una lezione sul disegno"
Tre atti: un disegno, un libro, una casa
2017
Il testo è scritto a tre mani. Esso riporta, in parti distinte, le testimonianze di tre azioni - il disegno, la scrittura e il progetto - intese come atti necessari al compimento dell’architettura. Un disegno (Parete, del 1976); un libro (Una lezione sul disegno, del 1996); una casa (Casa Pirrello, del 1990) sono presi a riferimento per descrivere una sezione parziale dell’opera autografa di Franco Purini. Al pari delle mani che scrivono, l’unità è raggiunta dalla complementarietà di ogni parte nel tutto.
Redox regulation of genome stability by effects on gene expression, epigenetic pathways and DNA damage/repair
2015
Reactive oxygen and nitrogen species (e.g. H2O2, nitric oxide) confer redox regulation of essential cellular signaling pathways such as cell differentiation, proliferation, migration and apoptosis. In addition, classical regulation of gene expression or activity, including gene transcription to RNA followed by translation to the protein level, by transcription factors (e.g. NF-κB, HIF-1α) and mRNA binding proteins (e.g. GAPDH, HuR) is subject to redox regulation. This review will give an update of recent discoveries in this field, and specifically highlight the impact of reactive oxygen and nitrogen species on DNA repair systems that contribute to genomic stability. Emphasis will be placed …
Oxidative DNA base damage induced by singlet oxygen and photosensitization: recognition by repair endonucleases and mutagenicity.
2000
We have analyzed the recognition by various repair endonucleases of DNA base modifications induced by three oxidants, viz. [4-(tert-butyldioxycarbonyl)benzyl]triethylammonium chloride (BCBT), a photochemical source of tert-butoxyl radicals, disodium salt of 1,4-etheno-2,3-benzodioxin-1,4-dipropanoic acid (NDPO(2)), a chemical source of singlet oxygen, and riboflavin, a type-I photosensitizer. The base modifications induced by BCBT, which were previously shown to be mostly 7,8-dihydro-8-oxoguanine (8-oxoGua) residues, were recognized by Fpg and Ogg1 proteins, but not by endonuclease IIII, Ntg1 and Ntg2 proteins. In the case of singlet oxygen induced damage, 8-oxoGua accounted for only 35% of…
Widespread transcriptional gene inactivation initiated by a repair intermediate of 8-oxoguanine.
2016
DNA damage can significantly modulate expression of the affected genes either by direct structural interference with transcription components or as a collateral outcome of cellular repair attempts. Thus, DNA glycosylases of the base excision repair (BER) pathway have been implicated in negative transcriptional response to several spontaneously generated DNA base modifications, including a common oxidative DNA base modification 8-oxoguanine (8-oxoG). Here, we report that single 8-oxoG situated in the non-transcribed DNA strand of a reporter gene has a pronounced negative effect on transcription, driven by promoters of various strength and with different structural properties, including viral…
The impact of ovarian stimulation on the expression of candidate reprogramming genes in mouse preimplantation embryos.
2012
Ovarian stimulation with gonadotrophins is an integral part of assisted reproductive technologies in human subfertility/infertility treatment. Recent findings have associated ovarian stimulation with the increased incidence of imprinting disorders in humans as well as defects in genome-wide methylation reprogramming and, in particular, imprinting in mice. Here, we present the first study that determined the impact of ovarian stimulation on the expression of developmentally important reprogramming genes <i>(Apex1, Lig1, Lig3, Mbd2, Mbd3, Mbd4, </i>and<i> Polb)</i> in single early mouse morula embryos (16-cell stage). Using absolute quantification of mRNA by quantitati…
Gibellina, la città che visse due volte : terremoto e ricostruzione nella Valle del Belice
2019
Il passato di Gibellina Nuova non è Gibellina Vecchia. Le storie delle due città sono profondamente diverse: il terremoto del 1968, che colpì con un’intensità del IX grado della scala Mercalli la Valle del Belice, è stato additato come causa della loro soluzione di continuità, ma i veri fattori che hanno determinato una frattura nella continuità storica vanno ricercati nelle politiche di intervento e nelle utopie urbanistiche che hanno orientato la ricostruzione. Il dopo-terremoto del Belice è stato il banco di prova della Prima Repubblica di fronte alle calamità naturali; dal fallimento delle sperimentazioni urbanistiche operate in vivo nel tessuto sociale, dalla dicotomia tra ricostruzion…
Apurinic acid, a modified DNA with anticancer activity
1971
Apurinsaure, ein purinfreies hochmolekulares Desoxyribonukleinsaure-Derivat, hemmt das Wachstum des menschlichen Kolontumors GW-77 und das amelanotsiche Melanom des Hamsters.