Search results for "Purl"

showing 10 items of 382 documents

Guidelines for the use and interpretation of assays for monitoring cell death in higher eukaryotes

2009

Cell death is essential for a plethora of physiological processes, and its deregulation characterizes numerous human diseases. Thus, the in-depth investigation of cell death and its mechanisms constitutes a formidable challenge for fundamental and applied biomedical research, and has tremendous implications for the development of novel therapeutic strategies. It is, therefore, of utmost importance to standardize the experimental procedures that identify dying and dead cells in cell cultures and/or in tissues, from model organisms and/or humans, in healthy and/or pathological scenarios. Thus far, dozens of methods have been proposed to quantify cell death-related parameters. However, no guid…

MESH: Cell DeathcytofluorometryMESH : Microscopy Fluorescenceved/biology.organism_classification_rank.speciesCellMESH: Flow CytometryMESH: Microscopy FluorescenceApoptosisfluorescence microscopyMESH: Eukaryotic CellsAnnexin Vnecrosis0302 clinical medicineEukaryotic Cells/cytologyMitochondrial membrane permeabilizationScanningMESH : ImmunoblottingGeneticsApoptosis; Cell Death; Eukaryotic Cells/cytology; Flow Cytometry; Guidelines as Topic; Humans; Immunoblotting; Microscopy Electron Scanning; Microscopy Fluorescence; Spectrometry Fluorescence0303 health sciencesMicroscopyMESH : Spectrometry FluorescenceMESH: ImmunoblottingCell DeathMESH: Guidelines as Topic//purl.org/becyt/ford/3.1 [https]Bioquímica y Biología MolecularFlow Cytometry3. Good healthTunelMedicina Básicamedicine.anatomical_structureEukaryotic Cellscaspases030220 oncology & carcinogenesis//purl.org/becyt/ford/3 [https]MESH: Spectrometry FluorescenceMESH : Microscopy Electron ScanningProgrammed cell deathautophagyCIENCIAS MÉDICAS Y DE LA SALUDMESH: Microscopy Electron ScanningMESH : Flow CytometrycaspaseImmunoblottingGuidelines as TopicComputational biologyBiologyElectronFluorescenceArticle03 medical and health sciencesSettore MED/04 - PATOLOGIA GENERALEmedicine[SDV.BBM] Life Sciences [q-bio]/Biochemistry Molecular BiologyHumans[SDV.BBM]Life Sciences [q-bio]/Biochemistry Molecular BiologyModel organismddc:612mitotic catastropheMolecular Biology[ SDV.BBM ] Life Sciences [q-bio]/Biochemistry Molecular BiologyMESH : Guidelines as Topic030304 developmental biologycell death; Apoptosis; caspase; autophagy; Oxidative stress; fluorescence microscopyMESH: Humansved/biologySpectrometryInterpretation (philosophy)MESH: ApoptosisMESH : Eukaryotic CellsMESH : HumansApoptosis; Eukaryotic Cells; Flow Cytometry; Guidelines as Topic; Humans; Immunoblotting; Microscopy Electron Scanning; Microscopy Fluorescence; Spectrometry Fluorescence; Cell Death; Molecular Biology; Cell Biologyimmunofluorescence microscopyCell BiologySpectrometry FluorescenceMicroscopy FluorescenceOxidative stressMESH : Cell DeathCancer cellMicroscopy Electron ScanningMESH : Apoptosis
researchProduct

The Missing Link in the Magnetism of Hybrid Cobalt Layered Hydroxides: The Odd‐Even Effect of the Organic Spacer

2021

A dramatic change in the magnetic behaviour, which solely depends on the parity of the organic linker molecules, has been found in a family of layered CoII hydroxides covalently functionalized with dicarboxylic molecules. These layered hybrid materials have been synthesized at room temperature using a one-pot procedure through the epoxide route. While hybrids connected by odd alkyl chains exhibit coercive fields (Hc) below ca. 3500 Oe and show spontaneous magnetization at temperatures (TM) below 20 K, hybrids functionalized with even alkyl chains behave as hard magnets with Hc>5500 Oe and display a TM higher than 55 K. This intriguing behaviour was studied by density functional theory with …

Magnetismchemistry.chemical_element010402 general chemistry01 natural sciencesMAGNETISMCatalysis//purl.org/becyt/ford/2.10 [https]MoleculeLAYERED HYDROXIDESMaterialsAlkylchemistry.chemical_classificationHYBRID MATERIALS010405 organic chemistry2D MATERIALSOrganic ChemistryGeneral Chemistryequipment and supplies0104 chemical sciencesCrystallographyDFT SIMULATIONSOctahedronchemistry//purl.org/becyt/ford/2 [https]Density functional theoryHybrid materialCobaltSpontaneous magnetizationhuman activitiesChemistry – A European Journal
researchProduct

Entanglement-Based dc magnetometry with separated ions

2017

We demonstrate sensing of inhomogeneous dc magnetic fields by employing entangled trapped ions, which are shuttled in a segmented Paul trap. As sensor states, we use Bell states of the type j↑↓i þ eiφj↓↑i encoded in two 40Caþ ions stored at different locations. The linear Zeeman effect leads to the accumulation of a relative phase φ, which serves for measuring the magnetic-field difference between the constituent locations. Common-mode magnetic-field fluctuations are rejected by the entangled sensor state, which gives rise to excellent sensitivity without employing dynamical decoupling and therefore enables accurate dc sensing. Consecutive measurements on sensor states encoded in the S1=2 g…

Magnetometeriones fríosQC1-999Ciencias FísicasGeneral Physics and AstronomyFOS: Physical sciences02 engineering and technologyQuantum entanglementmagnetometría01 natural scienceslaw.inventionIon//purl.org/becyt/ford/1 [https]computacion cuánticalaw0103 physical sciences010306 general physicsPhysicsCondensed Matter::Quantum GasesQuantum PhysicsCondensed matter physicsPhysics//purl.org/becyt/ford/1.3 [https]021001 nanoscience & nanotechnologyAstronomía0210 nano-technologyQuantum Physics (quant-ph)CIENCIAS NATURALES Y EXACTAS
researchProduct

Human fascioliasis endemic areas in Argentina: multigene characterisation of the lymnaeid vectors and climatic-environmental assessment of the transm…

2016

Background: In South America, fascioliasis stands out due to the human endemic areas in many countries. In Argentina, human endemic areas have recently been detected. Lymnaeid vectors were studied in two human endemic localities of Catamarca province: Locality A beside Taton and Rio Grande villages; Locality B close to Recreo town. Methods: Lymnaeids were characterised by the complete sequences of rDNA ITS-2 and ITS-1 and fragments of the mtDNA 16S and cox1. Shell morphometry was studied with the aid of a computer image analysis system. Climate analyses were made by nearest neighbour interpolation from FAO data. Koeppen & Budyko climate classifications were used. De Martonne aridity index a…

Male0301 basic medicineEntomologyClimaterDNADisease Vectorslaw.invention//purl.org/becyt/ford/1 [https]0302 clinical medicinelawEnvironmental impact assessmentPhylogenyLymnaeaLymnaea neotropicamtDNAEcologyLymnaea viatorVectors030108 mycology & parasitologyInfectious DiseasesTransmission (mechanics)Human fascioliasisFemaleCIENCIAS NATURALES Y EXACTASFascioliasisOtras Ciencias Biológicas030231 tropical medicineArgentinaEnvironmentBiologyDNA MitochondrialDNA RibosomalCiencias Biológicas03 medical and health sciencesDNA Ribosomal SpacerFASCIOLIASISAnimalsHumans//purl.org/becyt/ford/1.6 [https]Lymnaea viatorHuman fascioliasisHUMAN INFECTIONResearchMorphometrySequence Analysis DNALYMNAEAFasciola hepaticaHaplotypesCyclooxygenase 1ParasitologyLymnaea neotropicaParasites & Vectors
researchProduct

Early-infantile onset epilepsy and developmental delay caused by bi-allelic GAD1 variants

2020

Mice lacking GAD1 show neonatal mortality, but the human phenotype associated with GAD1 disruption is poorly characterized. Neuray et al. describe six patients with biallelic GAD1 mutations, presenting with early-infantile onset epilepsy, neurodevelopmental delay, muscle weakness and non-CNS manifestations.

Male0301 basic medicineGlutamate decarboxylaseMalalties cerebralsNeurotransmissorsNeurodevelopmental delayEpilepsy0302 clinical medicineMESH: ChildAge of OnsetChildcleft palateGAD1AcademicSubjects/SCI01870Glutamate DecarboxylaseGlutamate receptorMuscle weakness//purl.org/becyt/ford/3.1 [https]NeurotransmittersMESH: InfantHypotoniamuscle weakneCleft palateMESH: EpilepsyChild PreschoolMuscle Hypotonia[SDV.NEU]Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC]//purl.org/becyt/ford/3 [https]FemaleBrain diseasesAbnormalitiesmedicine.symptomMultiplemedicine.drugcleft palate; epilepsy; GAD1; muscle weakness; neurodevelopmental delayMESH: Glutamate Decarboxylasemedicine.medical_specialtyMESH: Abnormalities MultipleMESH: MutationMESH: Age of OnsetBiologyInhibitory postsynaptic potentialGAD1 cleft palate epilepsy muscle weakness neurodevelopmental delay.gamma-Aminobutyric acidGAD1neurodevelopmental delay03 medical and health sciencesExcitatory synapseInternal medicinemedicineHumansAbnormalities MultiplePreschoolAllelesMESH: Neurodevelopmental Disordersmuscle weaknessMESH: HumansEpilepsyMESH: Muscle HypotoniaMESH: AllelesMESH: Child PreschoolInfantmedicine.diseaseMESH: MaleEpilèpsiaEditor's Choice030104 developmental biologyEndocrinologyNeurodevelopmental DisordersMutationepilepsyAcademicSubjects/MED00310Neurology (clinical)Cleft palate; Epilepsy; GAD1; Muscle weakness; Neurodevelopmental delay; Abnormalities Multiple; Age of Onset; Alleles; Child; Child Preschool; Epilepsy; Female; Glutamate Decarboxylase; Humans; Infant; Male; Muscle Hypotonia; Mutation; Neurodevelopmental DisordersMESH: Female[SDV.MHEP]Life Sciences [q-bio]/Human health and pathology030217 neurology & neurosurgeryReports
researchProduct

Ex Vivo Treatment with a Polyphenol-Enriched Cocoa Extract Ameliorates Myocardial Infarct and Postischemic Mitochondrial Injury in Normotensive and H…

2016

Our objective was to determine the effects of a polyphenol-enriched cocoa extract (PCE) on myocardial postischemic alterations in normotensive (Wistar rats, W) and spontaneously hypertensive rats (SHR). Isolated hearts were submitted to 110 min of perfusion or 20 min stabilization, 30 min global ischemia, and 60 min reperfusion (R). Other hearts were treated with PCE at the onset of R. Infarct size, the reduced glutathione (GSH), and the expression of phospho-Akt, P-GSK-3β, and P-eNOS were assessed. In isolated mitochondria, the Ca2+-mediated response of mitochondrial permeability transition pore (mPTP), membrane potential (δψm), and superoxide production were determined. PCE decreased infa…

Male0301 basic medicineMyocardial InfarctionWistarBlood Pressure030204 cardiovascular system & hematologyPharmacologyMitochondrial Membrane Transport ProteinsInfarct sizeSHRGlycogen Synthase Kinase 3chemistry.chemical_compound0302 clinical medicineMITOCHONDRIAIschemiaSuperoxidesEnosRats Inbred SHRbiologySuperoxideMPTPINFARCT SIZEHeart//purl.org/becyt/ford/3.1 [https]GlutathioneMitochondriaMedicina BásicaHypertension//purl.org/becyt/ford/3 [https]General Agricultural and Biological SciencesPerfusionCocaCardiotonic AgentsCIENCIAS MÉDICAS Y DE LA SALUDInmunologíaIschemiaIn Vitro Techniques03 medical and health sciencesPOLYPHENOLSWISTARmedicineAnimalsHumansRats WistarSHR; Wistar; infarct size; mitochondria; polyphenolsMitochondrial Permeability Transition PorePlant ExtractsMyocardiumCocoa ExtractPolyphenolsGeneral ChemistryGlutathionemedicine.diseasebiology.organism_classificationRats030104 developmental biologychemistryMitochondrial permeability transition poreCiencias MédicasJournal of Agricultural and Food Chemistry
researchProduct

Breizacanthus aznari sp. n. (Acanthocephala: Arhythmacanthidae) from the banded cusk-eel Raneya brasiliensis (Ophidiiformes: Ophidiidae) from the Pat…

2012

Breizacanthus aznari sp. n. is described from the banded cusk-eel Raneya brasiliensis (Kaup) (Ophidiiformes: Ophidiidae) from the Patagonian coast in Argentina. Breizacanthus Golvan, 1969 is currently composed of five species (including the new species) and is characterised by the absence of trunk spines, a short cylindrical proboscis with two types of hooks and lemnisci longer than the proboscis receptacle. Breizacanthus aznari is clearly distinguished from B. chabaudi Golvan, 1969 by having 12 longitudinal rows of hooks on the proboscis, instead of 16-18. The new species resembles B. golvani Gaevskaya et Shukhgalter, 1984, B. irenae Golvan, 1969, and B. ligur Paggi, Orecchia et Della Seta…

MaleArhythmacanthidaePalaeacanthocephalaOtras Ciencias BiológicasArgentinaPalaeacanthocephalaOphidiiformesEuzetacanthusMarine fishAcanthocephalaCiencias Biológicas//purl.org/becyt/ford/1 [https]Fish DiseasesPatagoniaAnimalsSouthwestern Atlantic//purl.org/becyt/ford/1.6 [https]Atlantic OceanSouthern HemisphereTaxonomybiologyRaneya brasiliensisFishesSetaAnatomybiology.organism_classificationSpiny-headed wormsFemaleParasitologyTaxonomy (biology)Helminthiasis AnimalAcanthocephalaCIENCIAS NATURALES Y EXACTASFolia Parasitologica
researchProduct

Worldwide trends in diabetes since 1980: a pooled analysis of 751 population-based studies with 4.4 million participants

2016

Background One of the global targets for non-communicable diseases is to halt, by 2025, the rise in the age-standardised adult prevalence of diabetes at its 2010 levels. We aimed to estimate worldwide trends in diabetes, how likely it is for countries to achieve the global target, and how changes in prevalence, together with population growth and ageing, are affecting the number of adults with diabetes. Methods We pooled data from population-based studies that had collected data on diabetes through measurement of its biomarkers. We used a Bayesian hierarchical model to estimate trends in diabetes prevalence-defined as fasting plasma glucose of 7.0 mmol/L or higher, or history of diagnosis w…

MaleBlood GlucoseAdultResearch ReportSettore MED/09 - Medicina Internaglucosa sanguíneaEpidemiologyhumanosEpidemiology; prevention; public health; diabetescoste de las enfermedadesDiabetes epidemiologyGlobal HealthincidenciaWorldwide trendsdistribución por sexosAge DistributionSDG 3 - Good Health and Well-beingCost of Illnessdistribución por edadespreventionpaíses en desarrolloDiabetes MellitusPrevalenceInsulinHumansHypoglycemic AgentsinsulinaAge of OnsetSex DistributionDeveloping CountriesdiabetesVDP::Medical disciplines: 700::Clinical medical disciplines: 750Incidenceprevalenciapublic healthVDP::Medisinske Fag: 700::Klinisk medisinske fag: 750Bayes TheoremadultoHealth Surveysinforme de investigación//purl.org/pe-repo/ocde/ford#3.02.00 [https]hipoglicemiantesencuestas de saludFemalepooled analysisteorema de Bayes
researchProduct

Prevalence of sarcopenia and associated factors in the healthy older adults of the Peruvian Andes

2016

Aim To assess the prevalence of sarcopenia and associated factors in a population of older people living in a rural area of the Peruvian Andes. Materials and methods The study concerned 222 people aged ≥65 years. Sarcopenia was diagnosed on the basis of skeletal muscle mass, measured using bioimpedance analysis, and gait speed, measured with the 4-m walking test, as recommended by the International Working Group on sarcopenia. Self-reported physical activity, the Short Physical Performance Battery, and the Six-Minute Walking Test also contributed information on participants’ physical performance status. Disabilities were investigated by assessing participants’ self-reported difficulties in …

MaleGerontologySarcopeniaAgingHealth (social science)Activities of daily livingSarcopenia/epidemiologyPopulationNutritional StatusWalk Test030209 endocrinology & metabolismMotor activityBody Mass IndexNODisability Evaluation03 medical and health sciencesSex Factors0302 clinical medicinePeruPrevalenceInstrumental activities of daily livingHumansMedicineActivities of daily living Body mass index Instrumental activities of daily living Motor activity Sarcopenia030212 general & internal medicineeducationExerciseBody mass indexAgededucation.field_of_studybusiness.industryConfoundingAge FactorsActivities of daily livingOdds ratiomedicine.diseasePhysical activity levelConfidence intervalSarcopeniaFemaleGeriatrics and Gerontology//purl.org/pe-repo/ocde/ford#3.02.26 [https]businessPeru/epidemiologyhuman activitiesGerontologyBody mass indexActivities of daily living; Body mass index; Instrumental activities of daily living; Motor activity; SarcopeniaArchives of Gerontology and Geriatrics
researchProduct

The dapagliflozin and prevention of adverse outcomes in chronic kidney disease (DAPA-CKD) trial: baseline characteristics

2020

Abstract Background The Dapagliflozin and Prevention of Adverse outcomes in Chronic Kidney Disease (DAPA-CKD; NCT03036150) trial was designed to assess the effect of the sodium–glucose co-transporter 2 (SGLT2) inhibitor dapagliflozin on kidney and cardiovascular events in participants with CKD with and without type 2 diabetes (T2D). This analysis reports the baseline characteristics of those recruited, comparing them with those enrolled in other trials. Methods In DAPA-CKD, 4304 participants with a urinary albumin:creatinine ratio (UACR) ≥200 mg/g and estimated glomerular filtration rate (eGFR) between 25 and 75 mL/min/1.73 m2 were randomized to dapagliflozin 10 mg once daily or placebo. Me…

MaleIGA NEPHROPATHYRATIONALEPROGRESSIONType 2 diabetesurologic and male genital diseasesDiabetic nephropathychemistry.chemical_compoundGlucosidesDESIGNMedicineFAILUREDiabetic NephropathiesDapagliflozinrandomized controlled clinical trialPrognosisfemale genital diseases and pregnancy complications//purl.org/pe-repo/ocde/ford#3.02.13 [https]Cardiovascular DiseasesNephrologyFemalesodium–glucose co-transporter-2 inhibitormedicine.symptomBENAZEPRILGlomerular Filtration Ratemedicine.medical_specialtyFinerenoneINHIBITIONNephropathy//purl.org/pe-repo/ocde/ford#3.02.20 [https]Double-Blind MethodClinical ResearchInternal medicineDiabetes mellitusEND-POINTSHumansBenzhydryl CompoundsRenal Insufficiency ChronicAcademicSubjects/MED00340Sodium-Glucose Transporter 2 InhibitorsAgedTransplantationDECLINEbusiness.industrysodium-glucose co-transporter-2 inhibitordapagliflozinmedicine.diseaseEFFICACYEditor's ChoiceDiabetes Mellitus Type 2chemistryAlbuminuriaORIGINAL ARTICLESbusinesschronic kidney diseaseKidney disease
researchProduct