Search results for "Pyruvate"
showing 10 items of 134 documents
Energy Metabolism, Lactate/Pyruvate Ratio and Extracellular Space in Cortex and White Matter Adjacent to and Distant from a Local Freezing Lesion
1969
The present investigation was undertaken to study the distribution of pathological fluid accumulation and the local energy metabolism in the cortex and white matter following a localized traumatic lesion of the cortex. A systematic examination of the changes in the tissue following a trauma is the basis for further studies on the relationship between cortical blood supply, local tissue metabolism and local function.
Argan oil prevents down-regulation induced by endotoxin on liver fatty acid oxidation and gluconeogenesis and on peroxisome proliferator-activated re…
2015
In patients with sepsis, liver metabolism and its capacity to provide other organs with energetic substrates are impaired. This and many other pathophysiological changes seen in human patients are reproduced in mice injected with purified endotoxin (lipopolysaccharide, LPS). In the present study, down-regulation of genes involved in hepatic fatty acid oxidation (FAOx) and gluconeogenesis in mice exposed to LPS was challenged by nutritional intervention with Argan oil. Mice given a standard chow supplemented or not with either 6% (w/w) Argan oil (AO) or 6% (w/w) olive oil (OO) prior to exposure to LPS were explored for liver gene expressions assessed by mRNA transcript levels and/or enzyme a…
Zur Wirkung von Butazolidin im Intermedi�rstoffwechsel
1956
Butazolidin (Phenylbutazone) inhibits the oxidative decarboxylation of pyruvate and α-ketoglutarate in a final concentration of 10 mg-% (3,24 · 10−4 m). Data are presented suggesting that the β-ketothiolase is inhibited. The following enzymes or enzyme systems are not inhibited: The enzymes of the respiratory chain, the enzymes of the citric acid cycle with exception of α-ketoglutaric oxidase, the glycolysis of hexosediphosphate (slight inhibition), acetate thiokinase, sulfanilamid transacetylase, pyruvic decarboxylase from yeast, arginase, xanthine oxidase, and D-amino acid oxidase.
Dephosphorylation of Centrins by Protein Phosphatase 2C α and β.
2009
In the present study, we identified protein phosphatases dephosphorylating centrins previously phosphorylated by protein kinase CK2. The following phosphatases known to be present in the retina were tested: PP1, PP2A, PP2B, PP2C, PP5, and alkaline phosphatase. PP2C 𝛼 and 𝛽 were capable of dephosphorylating P-Thr138-centrin1 most efficiently. PP2C𝛿 was inactive and the other retinal phosphatases also had much less or no effect. Similar results were observed for centrins 2 and 4. Centrin3 was not a substrate for CK2. The results suggest PP2C 𝛼 and 𝛽 to play a significant role in regulating the phosphorylation status of centrins in vivo.
6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase from frog skeletal muscle: purification, kinetics and immunological properties.
1993
Fructose 2,6-bisphosphate is the most potent activator of 6-phosphofructo-1-kinase, a key regulatory enzyme of glycolysis in animal tissues. This study was prompted by the finding that the content of fructose 2,6-bisphosphate in frog skeletal muscle was dramatically increased at the initiation of exercise and was closely correlated with the glycolytic flux during exercise. 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase, the enzyme system catalyzing the synthesis and degradation of fructose 2,6-bisphosphate, was purified from frog (Rana esculenta) skeletal muscle and its properties were compared with those of the rat muscle type enzyme expressed in Escherichia coli using recombinant DN…
Modeling and Analysis of a New Process for Pyruvate Production
2005
Early process development is crucial for the economic and environmental performance of a final production process. A new process for the production of pyruvate is presented as a case study to show how process modeling and an analysis of the results regarding economic and environmental aspects improves the understanding of the process, its cost structure, and its environmental impact. It also enables the quantification of process improvements and the comparison of alternative designs. The model of the pyruvate production shows how strain and fermentation improvements propagate through the process and affect its economic and environmental performance. The comparison of two alternative purific…
Phospholipidosis and down-regulation of the PI3-K/PDK-1/Akt signalling pathway are vitamin E inhibitable events associated with 7-ketocholesterol-ind…
2007
International audience; Among the oxysterols accumulating in atherosclerotic plaque, 7-ketocholesterol (7KC) is a potent apoptotic inducer, which favours myelin figure formation and polar lipid accumulation. This investigation performed on U937 cells consisted in characterizing the myelin figure formation process; determining the effects of 7KC on the PI3-K/PDK-1/Akt signalling pathway; evaluating the activities of vitamin E (Vit-E) (α-tocopherol) on the formation of myelin figures and the PI3-K/PDK-1/Akt signalling pathway and assessing the effects of PI3-K inhibitors (LY-294002, 3-methyladenine) on the activity of Vit-E on cell death and polar lipid accumulation. The ultrastructural and b…
Identification of enolase as a plasminogen-binding protein in excretory-secretory products ofFasciola hepatica
2004
AbstractWe have followed a combined proteomic approach to identify proteins of Fasciola hepatica that could be involved in host–parasite interactions. Using two-dimensional gel electrophoresis, far Western immunoblot and mass spectrometry analyses, we have identified the enolase enzyme, present in the excretory/secretory materials of F. hepatica, as a human plasminogen-binding protein. This enzyme has an apparent molecular weight of 47 kDa with pI ranging from 6.2 to 7.2. These results suggest that enolase could act as a plasminogen receptor.
Regional differences of substrate oxidation capacity in rat hearts: effects of extra load and endurance training.
1990
Male rats, aged 17 weeks at the end of experiments, were divided into four groups. Two groups lived in normal cage conditions with or without extra load (20% of the body weight) and two groups were trained by running with or without extra load for 8 weeks. Oxidation rates of succinate, glutamate + malate, palmitoylcarnitine, and pyruvate, and the activities of lactate dehydrogenase, citrate synthase, isocitrate dehydrogenase and cytochrome oxidase were measured in homogenates of the right ventricle and in those of the subendocardial and subepicardial layers of the left ventricle. Oxidation rates of succinate and palmitoylcarnitine tended to be higher in the subendocardium than in the subepi…
Pyruvate kinase type M2: a crossroad in the tumor metabolome.
2002
Cell proliferation is a process that consumes large amounts of energy. A reduction in the nutrient supply can lead to cell death by ATP depletion, if cell proliferation is not limited. A key sensor for this regulation is the glycolytic enzyme pyruvate kinase, which determines whether glucose carbons are channelled to synthetic processes or used for glycolytic energy production. In unicellular organisms pyruvate kinase is regulated by ATP, ADP and AMP, by ribose 5-P, the precursor of the nucleic acid synthesis, and by the glycolytic intermediate fructose 1,6-P2 (FBP), thereby adapting cell proliferation to nutrient supply. The mammalian pyruvate kinase isoenzyme type M2 (M2-PK) displays the …