Search results for "RACE"

showing 10 items of 4458 documents

CCDC 919043: Experimental Crystal Structure Determination

2013

Related Article: María Castellano, Wdeson P. Barros, Alvaro Acosta, Miguel Julve, Francesc Lloret, Yanling Li, Yves Journaux, Giovanni De Munno, Donatella Armentano, Rafael Ruiz-García, Joan Cano|2014|Chem.-Eur.J.|20|13965|doi:10.1002/chem.201403987

Tetra-n-butylammonium bis(mu~2~-22'-((910-dioxo-910-dihydroanthracene-14-diyl)diimino)bis(oxoacetato))-di-copper(ii) acetonitrile diethyl ether solvateSpace GroupCrystallographyCrystal SystemCrystal StructureCell ParametersExperimental 3D Coordinates
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Nickel induces intracellular calcium mobilization and pathophysiological responses in human cultured airway epithelial cells.

2009

Abstract Environmental exposure to nickel is associated to respiratory disorders and potential toxicity in the lung but molecular mechanisms remain incompletely explored. The extracellular Ca 2+ -sensing receptor (CaSR) is widely distributed and may be activated by divalent cations. In this study, we investigated the presence of CaSR in human cultured airway epithelial cells and its activation by nickel. Nickel transiently increased intracellular calcium (−log EC 50  = 4.67 ± 0.06) in A549 and human bronchial epithelial cells as measured by epifluorescence microscopy. Nickel (20 μM)-induced calcium responses were reduced after thapsigargin or ryanodine exposure but not by Ca 2+ -free medium…

ThapsigarginInterleukin-1betachemistry.chemical_elementRespiratory MucosaBiologyCalciumToxicologyCalcium in biologychemistry.chemical_compoundNickelExtracellularHumansRNA Small InterferingCells CulturedA549 cellRyanodine receptorRyanodineTumor Necrosis Factor-alphaInositol trisphosphateEpithelial CellsGeneral MedicineEnvironmental exposureIntercellular Adhesion Molecule-1Cell biologychemistryMicroscopy FluorescenceType C PhospholipasesImmunologyGTP-Binding Protein alpha Subunits Gq-G11ThapsigarginCalciumReceptors Calcium-SensingChemico-biological interactions
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Role of three isoforms of phospholipase A2in capacitative calcium influx in human T-cells

2002

The present study was conducted on human Jurkat T-cell lines in order to elucidate the role of phospholipase A2 in capacitative calcium entry. We have employed thapsigargin (TG) that induces increases in [Ca2+]i by emptying the calcium pool of endoplasmic reticulum, followed by capacitative calcium entry. We designed a Ca2+ free/Ca2+ reintroduction (CFCR) protocol for the experiments, conducted in Ca2+-free medium. By employing CFCR protocol, we observed that addition of exogenous arachidonic acid (AA) stimulated TG-induced capacitative calcium influx. The liberation of endogenous AA and its autocrine action seems to be implicated during TG-induced capacitative calcium influx: TG potentiate…

ThapsigarginbiologyEndoplasmic reticulumchemistry.chemical_elementCalciumBiochemistryJurkat cellsCell biologychemistry.chemical_compoundPhospholipase A2chemistryExtracellularbiology.proteinLiberationArachidonic acidEuropean Journal of Biochemistry
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Highly Efficient Virus-induced Gene Silencing (VIGS) in California Poppy (Eschscholzia californica): An Evaluation of VIGS as a Strategy to Obtain Fu…

2007

†Background and Aims Eschscholzia californica (California poppy) is an emerging model plant for ‘evo‐devo’ studies from the basal eudicot clade of Papaveraceae. California poppy has a relatively small genome, a short life cycle and, most importantly, it is amenable for transformation. However, since this transformation protocol is time consuming, virus-induced gene silencing (VIGS) was evaluated as a fast method to obtain functional data for California poppy genes. †Methods Commercially available California poppy plants were infiltrated with Agrobacterium tumefaciens carrying the tobacco rattle virus plasmids pTRV1 and pTRV2. pTRV2 contained part of the eschscholzia Phytoene Desaturase (EcP…

The Evolution of Plant DevelopmentRegulation of gene expressionGeneticsPhytoene desaturaseEschscholziabiologyCalifornia Poppyfungifood and beveragesFlowersPlant ScienceAgrobacterium tumefaciensbiology.organism_classificationEschscholziaPlant VirusesPlant LeavesGene Expression Regulation PlantTobacco rattle virusBotanyPapaveraceaeGene silencingGene SilencingOxidoreductasesAnnals of Botany
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Guilty Knowledge- testi valheenpaljastustestinä : Tarina- proseduuria käyttäen saadut tulokset kahdella eri pisteytysmenetelmällä

1997

The Story- procedurevalheenpaljastusMemory Trace TestGuilty Knowledge -tekniikkaSkin Conductance ResponseLie detectionGuilty Knowledge TechniqueTarina- proseduuri
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La scoperta, sistemazione e conservazione della grande Iscrizione di Gortina, nell'isola di Creta (1884-1921): la protezione delle testimonianze e le…

2013

La scoperta e la conservazione della Grande Iscrizione di Gortina a Creta, da parte degli studiosi italiani, fra la fine del XIX sec. e i primi decenni del XX sec., rappresentò un grande riconoscimento scientifico-culturale per la giovane nazione italiana. Nel passato, l’isola di Creta era stata legata alla Repubblica di Venezia e anche questo rapporto favorì l’invio a Creta del giovane epigrafista F. Halbherr, il quale fra notevoli peripezie rinvenne, assieme all’epigrafista tedesco E. Fabricius, la famosa l’iscrizione nell’antica città di Gortina. L’iscrizione, fra le più antiche e complete finora conosciute in tutta Europa, conteneva le norme sulla famiglia, l’eredità e in generale i dir…

The discovery and preservation of the Great Inscription of Gortyna in Crete by Italian researchers in the late nineteenth century. and the first decades of the twentieth century. was an important cultural and scientific recognition for the young Italian nation. In the past the island of Crete had been tied to the Republic of Venice and this report also favored sending in Crete the young epigraphist F. Halbherr who among considerable vicissitudes came to together to the epigraphist german E. Fabricius the inclusion of the famous ancient city of Gortyna. The inscription among the oldest and most comprehensive so far known throughout Europe the rules contained on the family's legacy in general and the rights and duties of some of the people of the Minoan civilization (480-450 BC). The inscription was carved on blocks organic limestone a restored ancient building which later in Roman times was turned into odeon. The Laws of Gortyn were engraved on the remains of the wall forming the passageway in front of the steps of the odeon. The essay traces the events that led to the discovery and subsequent storage made by Halbherr especially with the help of archaeologist L. Pernier and finally with the help of architect E. Stefani who designed a modern cover on the top of the ambulatory of the segments containing the inscription. This coverage was made to perform during the First World War on the basis of the Italian project from the Greek Government in order to protect the membership from possible war damage but especially from vandalism. The roof brick clay of the Laws of Gortyna still serves its function and ensures the use of the monument bearing witness to the preservation of Italian culture in Greece and more generally in the world. But in Sicily in the remains of the Villa del Casale in Piazza Armerina (Enna) its elegant protection (built in 1957) is representative of a whole period of culture of the Italian restoration is removed and replaced because it has not been able to make a ongoing maintenance is due. A bad example of conservation and management of a UNESCO monument from which arise after the work for the construction of new roofs an equally insulting to the archaeological heritage and scenic Italian and world.Settore ICAR/19 - Restauro
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DECORIN EFFECTS ON PROTEOMIC PROFILING OF BREAST CANCER CELLS: AN UPDATED STUDY

2015

The malignant carcinomas are characterized by several capabilities acquired by the neoplastic cells, among which the ability to invade the extracellular matrix (ECM) and to establish a crosstalk with several ECM components. Under this respect, the extracellular microenvironment is an entity extraordinarily rich of information with opposite signals. Our group has long undertaken the study of the effects of ECM molecules on the behavior of cancer cells in vitro. Among the studied molecules, the decorin was found to exert a non-permissive effect on the growth and motility of the transfected tumor cells. The decorin, belongs to the family of small leucine-rich proteoglycans (SLRP) and is involv…

The malignant carcinomas are characterized by several capabilities acquired by the neoplastic cells among which the ability to invade the extracellular matrix (ECM) and to establish a crosstalk with several ECM components. Under this respect the extracellular microenvironment is an entity extraordinarily rich of information with opposite signals. Our group has long undertaken the study of the effects of ECM molecules on the behavior of cancer cells in vitro. Among the studied molecules the decorin was found to exert a non-permissive effect on the growth and motility of the transfected tumor cells. The decorin belongs to the family of small leucine-rich proteoglycans (SLRP) and is involved physiologically in the fibrillogenesis of collagen. In the last few year a new anti-oncogenic role has been proposed for decorin1. This study aimed to implement the knowledge on the effects of ectopic decorin on breast cancer cells using as a reference point the results already achieved by our research group2 on the experimental model format. By breast cancer cell line 8701-BC and its transfected clone DEC-C2. The extension of the proteomic analysis combined with the mass spectrometry allowed to triplicate the number of identified proteins in our model. Among the newly identified proteins were members of the classes of metabolic enzymes S100 family and cell motility proteins which revealed a net decrease in the decorin transfected cells. Of considerable importance is the observation that these classes of proteins are the most involved in metastatic progression. These results confirm and reinforce the anti-oncogenic role hypothesized for decorin. The work was co-funded by the Italian 5x1000 to COBS.DECORIN
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Traducción y recepción: Rosalía de Castro en Italia

2020

El panorama de la recepción italiana contemporánea de los poetas españoles del siglo XIX, a través de las traducciones, no resulta especialmente satisfactorio, puesto que hay que esperar el siglo siguiente para encontrar los primeros estudios monográficos y ediciones de textos en traducción, aunque en forma antológica. Ello, no obstante, así como en el resto de Europa, el corpus de las publicaciones periódicas de las revistas ofrece un canal editorial muy proficuo para la recepción de textos literarios extranjeros. Este trabajo se centra en la presencia de la obra de Rosalía de Castro en la prensa italiana contemporánea. Al mismo tiempo, se ha intentado completar el estudio de la recepción …

The panorama of the contemporary Italian reception of the Spanish poets of the 19th century through translations is not particularly satisfactory since it is necessary to wait for the following century to find the first monographic studies and editions of texts in translation although in anthological form. However as in the rest of Europe the corpus of periodicals of magazines offers a very profitable editorial channel for receiving foreign literary texts. This work focuses on the presence of Rosalía de Castro's work in the contemporary Italian press. At the same time attempts have been made to complete the study of the reception of the Galician poet in Italy collecting traces in other editorial products such as the book. The consequent analytical focus will correspond to the transnational study of some proto and meta texts individualized by their special interest.
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Analyses of the TRACE V5 capability for the simulation of natural circulation and primary/containment coupling in BDBA condition typical of the MASLWR

2014

In the short term period the use of advanced Small Modular Reactor (SMR) is one of the most promising options for the deployment of nuclear technology. The validation and assessment of the best estimate thermal hydraulic system code TRACE against SMR thermal hydraulic phenomena is a novel effort. In this framework the use of the natural circulation database developed at the OSU-MASLWR test facility, simulating the MASLWR reactor prototype, is of interest for analyses of the TRACE code capability in predicting natural circulation and primary/containment coupled behavior in SMR. The target of this paper is to analyze the TRACE V5 capability for the simulation of natural circulation phenomena,…

Thermal hydraulicsEngineeringNatural circulationPrimary (chemistry)ContainmentCoupling (computer programming)business.industryNuclear engineeringMass flow ratebusinessSimulationTRACE (psycholinguistics)Small modular reactor
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Non-isothermal thermogravimetry as an accelerated tool for the shelf-life prediction of paracetamol formulations

2021

Abstract In this work, non-isothermal thermogravimetric studies have been carried out on several paracetamol formulations with the aim to predict their shelf-lives under variable storage conditions. Specifically, paracetamol tablets of different brands have been investigated allowing to estimate their pharmaceutical quality by considering the specific drug stability. The proposed protocol is based on the kinetic study of thermogravimetric data by the combination of isoconversional procedures (Friedman and Kissinger-Akahira-Sunose (KAS) methods) and “Master plot” analysis. Accordingly, the kinetics of the paracetamol degradation is totally explored in terms of activation energy, pre-exponent…

Thermogravimetric analysisMaterials scienceShelf-lifeThermodynamicsExpiration date02 engineering and technology021001 nanoscience & nanotechnologyCondensed Matter PhysicsShelf life01 natural sciencesIsothermal process010406 physical chemistry0104 chemical sciencesThermogravimetryDecay timeParacetamolIsoconversional methodsThermogravimetryPhysical and Theoretical Chemistry0210 nano-technologyInstrumentationSettore CHIM/02 - Chimica Fisica
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