Search results for "RACE"
showing 10 items of 4458 documents
Influence of extracellular matrix proteins on the development of cultured human dendritic cells.
1998
The development of dendritic cells (DC) is still only partly understood. Recently established culture systems using CD34+ cells or monocytes as precursor cells for the generation of DC indicate the necessity of pro-inflammatory cytokines for their development. In vivo the contact to other cells or to the proteins of the extracellular matrix might also be essential for their development. In our experiments we used granulocyte-macrophage colony-stimulating factor- and IL-4-treated human monocytes as precursor cells to investigate the interaction of DC at different maturation stages with the matrix proteins fibronectin, collagen type I and collagen type IV. We demonstrate a strong beta1-integr…
DNA fragmentation index, pAKT and pERK1/2 in cumulus cells are related to oocyte competence in patients undergoing in vitro fertilization programme
2019
SummaryActivated pERK1/2 and pAKT are key players in supporting cell survival and proliferation pathways. Translocation of pERK1/2 into the nucleus, where it interacts with transcription factors and DNA itself, is instrumental in exerting an anti-apoptotic effect. In this study, pAKT levels, pERK1/2 nuclear localization and DNA fragmentation index (DFI) in cumulus cells of single cumulus–oocyte complexes of patients undergoing in vitro fertilization programmes were evaluated and correlated with the clinical outcome of the related embryos. For a positive clinical outcome of blastocyst development, pERK1/2 nuclear localization and DFI value had a significant inverse relationship, whereas the …
On the Ultrastructure and Function of Rhogocytes from the Pond Snail Lymnaea stagnalis
2015
Rhogocytes, also termed “pore cells”, occur as solitary or clustered cells in the connective tissue of gastropod molluscs. Rhogocytes possess an enveloping lamina of extracellular matrix and enigmatic extracellular lacunae bridged by cytoplasmic bars that form 20 nm diaphragmatic slits likely to act as a molecular sieve. Recent papers highlight the embryogenesis and ultrastructure of these cells, and their role in heavy metal detoxification. Rhogocytes are the site of hemocyanin or hemoglobin biosynthesis in gastropods. Based on electron microscopy, we recently proposed a possible pathway of hemoglobin exocytosis through the slit apparatus, and provided molecular evidence of a common phylog…
Temporomandibular joint arthrocentesis. Review of the literature.
2011
The treatment of the temporomandibular joint (TMJ) is still controversial. TMJ arthrocentesis represents a form of minimally invasive surgical treatment in patients suffering from internal derangement of the TMJ, especially closed lock. It consists of washing the joint with the possibility of depositing a drug or other therapeutic substance. Resolution of symptoms is due to the removal of chemical inflammatory mediators and changes in intra-articular pressure. Numerous clinical studies regarding this technique have been published. The goal of this paper is to review all clinical articles that have been published with regard to the critique of this technique. 19 articles with different desig…
Intrahematomal Ultrasound Enhances RtPA-Fibrinolysis in a Porcine Model of Intracerebral Hemorrhage
2021
Catheter-based ultrasound-thrombolysis has been successfully used in a small clinical trial in order to enhance recombinant tissue plasminogen activator (rtPA)-fibrinolysis, for the treatment of spontaneous intracerebral hemorrhages (ICHs). The aim of this study was to investigate the ultra-early effects of ultrasound on hematoma and the surrounding brain tissue in a porcine ICH-model. To achieve this, 21 pigs with a right frontal ICH were randomly assigned to four groups: (1) drainage (n = 3), (2) drainage + rtPA (n = 6), (3) drainage + ultrasound (n = 6), and (4) drainage + ultrasound + rtPA (n = 6). The hematoma volume assessment was performed using cranial MRI before and after the treat…
Full scale evaluation of atmospheric mercury budget from persistent volcanic degassing
2009
In the recent years, there has been an increase in environmental concerns related to the pollution of many trace metals emitted from volcanic degassing because of their toxicity and long residence times in the earth’s ecosystems. Among volcanogenic trace elements, mercury (Hg) is one of the most environmentally-significant in light of its potential harmful effects on biological systems. At present, however, the global Hg flux from volcanic sources is still poorly known. We report on Hg levels in volcanic plumes and fumaroles that, in combination with sulphur analyses and SO2 flux data, lead to global Hg estimates. We found that mercury transport in the plume is mainly in the gas-phase, as H…
Analytical model for the long- distance tracer-transport in plants
2011
International audience; Recent investigations of long-distance transport in plants using non-invasive tracer techniques such as C radiolabeling monitored by positron emission tomography (PET) combined with magnetic resonance imaging (MRI) revealed the need of dedicated methods to allow a quantitative data analysis and comparison of such experiments. A mechanistic compartmental tracer transport model is presented, defined by a linear system of partial differential equations (PDEs). This model simplifies the complexity of axial transport and lateral exchanges in the transport pathways of plants (e.g. the phloem) by simulating transport and reversible exchange within three compartments using j…
Datizraces iespēju pielietojums studentu atbiruma prognozēšanai
2016
Bakalaurā darbā “Datizraces iespēju pielietojums studentu atbiruma prognozēšanai” ir apskatīti dažādi datizraces metožu teorētiskie aspekti un pamatlietas, kā arī veikts pētījums ar datizraces metodes pielietojumu, lai iegūtu praktisko pieredzi datu analizēšanā. Galvenais pētījuma mērķis bija izskaidrot, kāpēc Latvijas Universitātes “Datorzinātnes” bakalaura programmas un “Programmēšana un datortīklu administrēšana” programmas studenti pēc pirmā studiju gada vai pat pēc pirma semestra pamet studijas. Lai sasniegtu analīzes galveno mērķi, tika sagatavoti un atbilstoši apstrādāti dati, kas tika analizēti, izmantojot datizraces rīku WEKA, iegūtie rezultāti tika izpētīti un aprakstīti, uz to pa…
Consequences of analgesics use in early pregnancy: Results of tests on mice.
2019
International audience; Self-medication during pregnancy continues to be an issue in developing countries due topoor medical education. The most commonly used drugs are analgesics, mainlyacetaminophen (paracetamol, APAP) and, to a lesser extent, ketoprofen (KPF). The aim of thestudy was to establish whether there are consequences of accidental use of these two drugsduring early embryogenesis. The experimental study was performed on 30 pregnant whitemice, divided into three groups: a first group to which APAP was administered, a secondgroup to which KPF was administered, and group 3 as a control group. At delivery, the babymice were examined, and after their first parturition, they were take…
The vesicular transfer of CLIC1 from glioblastoma to microvascular endothelial cells requires TRPM7.
2018
Chloride intracellular channel 1 (CLIC1) is highly expressed and secreted by human glioblastoma cells and cell lines such as U87, initiating cell migration and tumor growth. Here, we examined whether CLIC1 could be transferred to human primary microvascular endothelial cells (HMEC). We previously reported that the oncogenic microRNA, miR-5096, increased the release of extracellular vesicles (EVs) by which it increased its own transfer from U87 to surrounding cells. Thus, we also examined its effect on the CLIC1 transfer. In homotypic cultures, miR-5096 did not increase the expression of CLIC1 in U87 nor in HMEC. However, the endothelial CLIC1 level increased after exposure to EVs released b…