Search results for "RAV"

Reactive oxygen species mediate angiotensin II-induced leukocyte-endothelial cell interactions in vivo

2001

Abstract Chronically elevated angiotensin II (Ang-II)-induced hypertension is partly mediated by superoxide production. In this study, we have investigated whether the leukocyte-endothelial cell interactions elicited by Ang-II involve reactive oxygen species (ROS) generation. Intravital microscopy within the rat mesenteric microvessels was used. Superfusion (60 min) with Ang-II (1 nM) induced significant increases in leukocyte rolling flux, adhesion, and emigration, which were inhibited by pretreatment with superoxide dismutase or catalase. Dihydrorhodamine-123 oxidation indicated that ROS are primarily produced by the vessel wall. Administration of dimethylthiourea, desferrioxamine, or N-a…

Intravital fluorescence microscopy for the study of leukocyte interaction with platelets and endothelial cells

1999

Publisher Summary This chapter introduces the techniques of intravital microscopy as a tool to study the microcirculation in intact animals under conditions of oxidative stress. Intravital fluorescence microscopy can be performed with almost all types of epiillumination microscopes available. The introduction of fluorescent dyes and refined epiillumination techniques has significantly advanced the possibilities of intravital microscopic studies of the liver microcirculation, including quantitative analyses of both circulatory parameters and cellular mechanisms. Using appropriate dyes, intravital microscopy allows the study of (1) microvascular perfusion, (2) leukocyte-endothelial cell inter…

2013

Re-canalization of cerebral vessels in ischemic stroke is pivotal to rescue dysfunctional brain areas that are exposed to moderate hypoxia within the penumbra from irreversible cell death. Goal of the present study was to evaluate the effect of moderate hypoxia followed by reoxygenation (MHR) on the evolution of reactive oxygen species (ROS) and blood-brain barrier (BBB) integrity in brain endothelial cells (BEC). BBB integrity was assessed in BEC in vitro and in microvessels of the guinea pig whole brain in situ preparation. Probes were exposed to MHR (2 hours 67-70 mmHg O2, 3 hours reoxygenation, BEC) or towards occlusion of the arteria cerebri media (MCAO) with or without subsequent repe…

Classification of vegetable oils according to their botanical origin using amino acid profiles established by High Performance Liquid Chromatography …

2010

Abstract A preliminary study using amino acid profiles to classify oils according to their botanical origin has been performed. Amino acid profiles were obtained from hydrolysis of proteins present in vegetable oils, and established by High Performance Liquid Chromatography (HPLC) with UV–vis detection. Proteins present in hazelnut, corn, soybean, olive, avocado, peanut and grapeseed oils were precipitated with acetone, and the residue was hydrolysed in acid medium. The amino acids obtained were derivatized with o -phthaldialdehyde and separated by HPLC. Peaks corresponding to 18 amino acids were observed using a C18 column and a gradient of acetonitrile–water in the presence of a 5 mM citr…

Solvent-Free Synthesis of a Pillared Three-Dimensional Coordination Polymer with Magnetic Ordering

2015

A new magnetic coordination polymer, [Fe(bipy)(im)2] (bipy = 4,4-bipyridine and im = imidazole), has been synthesized in a solvent-free reaction. Structural analysis reveals a pillared 3D coordination polymer composed by neutral layers, formed by iron(II) and imidazolate linkers, interconnected by bipy ligands which serve as pillars. Magnetic measurements show that the material magnetically orders at low temperatures (Tc = 14.5 K) as a weak ferromagnet, likely due to a spin canting.

The production of resveratrol (3,5,4'‐trihydroxystilbene) by grapevinein vitrocultures, and its application to screening for grey mould resistance

1992

The ability of grapevine in vitro cultures to synthesise the phytoalexin precursor resveratrol in response to ultraviolet light irradiations is investigated in order to develop methods for screening for resistance to grey mould (Botrytis cinerea Pers.) in grapevines produced as a result of somatic embryogenesis. It is demonstrated that resveratrol formation can be reproducibly induced in leaves of in vitro plantlets. Differences in the production of resveratrol among the three varieties of Vitis vinifera tested (Pinot Noir, Chardonnay and Cabernet Sauvignon) were consistent with observations regarding their field susceptibility to grey mould. The use of phytoalexin induction and of in vitro…

ChemInform Abstract: Eco-Friendly Functionalization of Natural Halloysite Clay Nanotube with Ionic Liquids by Microwave Irradiation for Suzuki Coupli…

2014

Abstract Microwave assisted halloysite (HNT) external surface functionalization with ionic liquids is described. HNTs modification was achieved in two steps: a) grafting of 3-mercaptopropyl trimethoxysilane on the external surface of HNT by a microwave irradiation; b) anchorage of vinylimidazolium ionic liquids by a thiol-ene reaction. MW irradiation allowed us to obtain high loading onto the HNT surface compared to those obtained through conventional synthesis. Fourier transform infrared spectroscopy and thermogravimetric analysis confirmed that the grafting has occurred only on the external surface of HNT. Turbidimetric and dynamic light scattering analyses showed that the introduction of…

Eco-friendly functionalization of natural halloysite clay nanotube with ionic liquids by microwave irradiation for Suzuki coupling reaction

2014

Abstract Microwave assisted halloysite (HNT) external surface functionalization with ionic liquids is described. HNTs modification was achieved in two steps: a) grafting of 3-mercaptopropyl trimethoxysilane on the external surface of HNT by a microwave irradiation; b) anchorage of vinylimidazolium ionic liquids by a thiol-ene reaction. MW irradiation allowed us to obtain high loading onto the HNT surface compared to those obtained through conventional synthesis. Fourier transform infrared spectroscopy and thermogravimetric analysis confirmed that the grafting has occurred only on the external surface of HNT. Turbidimetric and dynamic light scattering analyses showed that the introduction of…

Synthesis of new soluble polybenzyls by Friedel-Crafts reactions

1999

Soluble polybenzyls were prepared by a catalytic electrophilic Friedel-Crafts type polycondensation between α,α'-dichloro-p-xylene and substituted (by an alkyl or an alkoxy side-chain) mesitylene. The influence of the length of the side-chain on the solubility of the polymer was examined. The polymers were found to be soluble in common organic solvents. Polymer characterizations were made by 13 C nuclear magnetic resonance spectroscopy (NMR), size exclusion chromatography (SEC), differential scanning calorimetry (DSC), thermogravimetric analysis (TGA) and elemental analysis.

Thermogravimetric and calorimetric studies of water absorbed in polyaniline

1995

Abstract Thermogravimetric analysis (TGA) and differential scanning calorimetry (DSC) were used to study the behavior of residual water absorbed by an emeraldine base and a salt of chemically synthesized polyaniline (PANI—EB, PANI-ES). The matrix of PANI is shown to contain water molecules participating in both reversible and irreversible absorption. In its turn, the reversibly absorbed water consists of two components. The first component (WD 1 ) amounts to 5–7 wt.% and is removable from PANI-EB in a flow of dry nitrogen at room temperature. The second one (6–8 wt.%) of more strongly bonded water (WD 2 ) can be withdrawn from PANI-EB at temperatures from 70 to 150 °C depending on the measu…