Search results for "REGION"
showing 10 items of 4910 documents
HSP70 gene expression in Mytilus galloprovincialis hemocytes is triggered by moderate heat shock and Vibrio anguillarum, but not by V. splendidus or …
2005
Complete sequence of HSP70 cDNA from the mussel, Mytilus galloprovincialis was established before quantifying its expression following moderate heat shock or injection of heat-killed bacteria. HSP70 cDNA is comprised of 2378 bp including one ORF of 654 aa, with a predicted 70 bp 5'-UTR and a 343 bp 3'-UTR (GenBank, 18 Jan 05, AY861684). Alignment identity ranged from 89% for Crassostrea ariakensis to 72% for C. virginica. Curiously, HSP70 gene and cDNA sequences from M. galloprovincialis, deposited later (03 and 27 May), show only 73% identity with the present sequence. Meanwhile, characteristic motifs of the HSP70 family were located in conserved positions. Expression of HSP70 gene was qua…
Polymorphisms of β-defensin genes in Valle del Belice dairy sheep
2011
The aim of this work was to study β-defensin 1 (SBD1) and β-defensin 2 (SBD2) genes in Valle del Belice dairy sheep in order to identify polymorphisms that can be utilized as markers of the analyzed genes, and search for the functional effects and roles of the identified polymorphisms (variation of the amino acid sequence of the protein and stability of mRNA molecule). The study was conducted on 300 randomly selected animals belonging to four flocks. A total of seven SNPs were identified, two in SBD1 and five in SBD2. The two SNPs identified in SBD2 coding region, at position 1659 and position 1667, were non-synonymous, leading to amino acid changes in the protein product. Nevertheless, the…
Design of novel small molecule base-pair recognizers of toxic CUG RNA transcripts characteristics of DM1.
2020
Graphical abstract
Myotonic dystrophy associated expanded CUG repeat muscleblind positive ribonuclear foci are not toxic to Drosophila
2005
Myotonic dystrophy type 1 is an autosomal dominant disorder associated with the expansion of a CTG repeat in the 3 0 untranslated region (UTR) of the DMPK gene. Recent data suggest that pathogenesis is predominantly mediated by a gain of function of the mutant transcript. In patients, these expanded CUG repeat-containing transcripts are sequestered into ribonuclear foci that also contain the muscleblind-like proteins. To provide further insights into muscleblind function and the pathogenesis of myotonic dystrophy, we generated Drosophila incorporating CTG repeats in the 3 0 -UTR of a reporter gene. As in patients, expanded CUG repeats form discrete ribonuclear foci in Drosophila muscle cell…
The pro-opiomelanocortin gene of the zebrafish (Danio rerio)
2003
The cDNA and the gene for pro-opiomelanocortin (POMC) in the zebrafish (Danio rerio) were isolated and analyzed. The gene consists of three exons and two short introns and has a similar overall structural organization as in Homo sapiens. Intron 1 (339 bp) divides the 5(') untranslated region from the coding region while intron 2 (1522 bp) is located between the signal peptide and the sequence encoding ACTH. Transcription starts 26 bp downstream of a TATA box and there is one polyadenylation signal in the 3(') untranslated region. The cDNA comprises of 964 bp with an open reading frame encoding a 222 amino acid hormone prepropeptide that is split into six putative hormones. Sequence comparis…
miR-15a-3p Protects Against Isoniazid-Induced Liver Injury via Suppressing N-Acetyltransferase 2 Expression
2021
Isoniazid (INH), an effective first-line drug for tuberculosis treatment, has been reported to be associated with hepatotoxicity for decades, but the underlying mechanisms are poorly understood. N-acetyltransferase 2 (NAT2) is a Phase II enzyme that specifically catalyzes the acetylation of INH, and NAT2 expression/activity play pivotal roles in INH metabolism, drug efficacy, and toxicity. In this study, we systematically investigated the regulatory roles of microRNA (miRNA) in NAT2 expression and INH-induced liver injury via a series of in silico, in vitro, and in vivo analyses. Four mature miRNAs, including hsa-miR-15a-3p, hsa-miR-628-5p, hsa-miR-1262, and hsa-miR-3132, were predicted to …
Kim jestem? Identyfikacje i tożsamości społeczne : przypadek Górnego Śląska
2018
For centuries, identity has been an important existential issue, because it organizes human relation with the world. Identity is not only one man's being in a biological sense, but the principle of social and cultural order. The identity is the self-awareness, the result of the social actor's involvement in a variety of network connections which are forming a human being together with the biological dimension. Thus, the identity, on the one hand, is a kind of Erikson's tradition, namely the sense of being human, on the other hand interactionist tradition, modification of identity through a process of interaction. Contemporary world, the world of confronting cultures, raises the need for ana…
Dyskurs o Ślązakach
2019
The author of the article presents comments and postulates regarding research on the culture and history of Silesia, which were announced by representatives of various scientific disciplines in Poland after the Second World War. The philologist and cultural expert appealed for extensive scientific competence of the researcher of Silesian affairs, for understanding of the difficult realities of this land, and he warned against the diagnosis of local culture preached to a political or social order of an environment with specific views on the reality of the region. It is important that the author appealed for modeling the interpretation and analytical workshop, which for the research of this e…
The Impact of Culture and Creativity on the Economy of the City
2019
Abstract Creativity is an important component of the economy for modern cities, and necessary condition for it is the qualitative understanding of the economic theory science. In the global competition, it plays an important role for the economic growth dynamics, which is directly related to human resource role in the modern economy. The competitiveness of the territories depends on their attractiveness to economically active individuals and the opportunities they provide with economic growth. When considering the creative and cultural industries as potential economic development factors in the urban economy, culture can be considered as a new stage in urban development based on quality of …
Agrarian development and state building in Spain: the contest for irrigation in the Valencian Region, 1770–1860
2022
AbstractA determined expansion in the productive capacity of Spanish agriculture was a fundamental and contentious objective during the crisis of the country’s ancien régime and the formation of the liberal state, in the years of transition from the eighteenth to the nineteenth centuries. In this study we examine a fundamental reorientation that occurred in an ambitious project for the expansion of irrigation in the region of Valencia. In this region, characterised by a well-rooted commercial agriculture, the original scheme, initiated by an enlightened aristocrat well connected with the royal court, would be profoundly altered in the transition from one political regime to another. The irr…