Search results for "REGULATION"

Cutting Edge: An IL-17F-CreEYFP Reporter Mouse Allows Fate Mapping of Th17 Cells

2009

Abstract The need for reporter lines able to faithfully track Th17 cells in vivo has become an issue of exceptional importance. To address this, we generated a mouse strain in which Cre recombinase is expressed from the IL-17F promoter. Crossing the IL-17F-Cre allele to a conditional enhanced yellow fluorescent protein (EYFP) reporter mouse yielded the IL-17F-CreEYFP strain, in which IL-17F expression is twinned with EYFP in live IL-17F-expressing cells. Although we demonstrate that IL-17F expression is restricted to CD4+ T cells during experimental autoimmune encephalomyelitis, IL-17F-CreEYFP CD8 T cells robustly expressed IL-17F in response to TGF-β, IL-6, and IL-23. Fate mapping of IL-17…

Immunomodulatory effects of dietary β-1,3-glucan from Euglena gracilis in rainbow trout (Oncorhynchus mykiss) immersion vaccinated against Yersinia r…

2012

Abstract Potential immunostimulatory effects of orally administered β-glucan were investigated in combination with immersion vaccination against enteric redmouth disease caused by Yersinia ruckeri in rainbow trout (Oncorhynchus mykiss). A linear, unbranched and pure (purity ≥98%) β-1,3-glucan (syn. paramylon) from the alga Euglena gracilis was applied at an inclusion level of 1% β-glucan in feed administered at a rate of 1% biomass day−1 for 84 consecutive days. Fish were vaccinated after two weeks of experimental feeding and bath challenged with live Y. ruckeri six weeks post-vaccination. Blood and head kidney were sampled at day 0, 13 (1 day pre-vaccination), 15, 55, 59 (day 3 post-challe…

The influence of attachment on well-being: the mediating role of emotion regulation in Spanish youth

2021

Las personas construyen y mantienen vínculos emocionales a lo largo de todo su desarrollo vital. El modo de vincularse, es decir, el estilo de apego, es consecuencia de los modelos mentales de relación construidos durante las experiencias afectivas. Los individuos con apego inseguro informan de elevada ansiedad y/o elevada evitación al establecer relaciones interpersonales. Estas personas podrían beneficiarse especialmente del aprendizaje de estrategias adecuadas para regular sus emociones, y así, aumentar su grado de bienestar. El objetivo del presente trabajo es estudiar la relación entre el apego y el bienestar subjetivo en jóvenes españoles, contemplando la regulación emocional como var…

Alternative splicing regulation by Muscleblind proteins: from development to disease.

2011

Regulated use of exons in pre-mRNAs, a process known as alternative splicing, strongly contributes to proteome diversity. Alternative splicing is finely regulated by factors that bind specific sequences within the precursor mRNAs. Members of the Muscleblind (Mbl) family of splicing factors control critical exon use changes during the development of specific tissues, particularly heart and skeletal muscle. Muscleblind homologs are only found in metazoans from Nematoda to mammals. Splicing targets and recognition mechanisms are also conserved through evolution. In this recognition, Muscleblind CCCH-type zinc finger domains bind to intronic motifs in pre-mRNA targets in which the protein can e…

Kinetic modelling of the Zymomonas mobilis Entner-Doudoroff pathway: insights into control and functionality.

2013

Zymomonas mobilis, an ethanol-producing bacterium, possesses the Entner-Doudoroff (E-D) pathway, pyruvate decarboxylase and two alcohol dehydrogenase isoenzymes for the fermentative production of ethanol and carbon dioxide from glucose. Using available kinetic parameters, we have developed a kinetic model that incorporates the enzymic reactions of the E-D pathway, both alcohol dehydrogenases, transport reactions and reactions related to ATP metabolism. After optimizing the reaction parameters within likely physiological limits, the resulting kinetic model was capable of simulating glycolysis in vivo and in cell-free extracts with good agreement with the fluxes and steady-state intermediate …

Acyl-homoserine lactone production is more common among plant-associated Pseudomonas spp. than among soilborne Pseudomonas spp.

2001

ABSTRACT A total of 137 soilborne and plant-associated bacterial strains belonging to different Pseudomonas species were tested for their ability to synthesize N -acyl-homoserine lactones (NAHL). Fifty-four strains synthesized NAHL. Interestingly, NAHL production appears to be more common among plant-associated than among soilborne Pseudomonas spp. Indeed, 40% of the analyzed Pseudomonas syringae strains produced NAHL which were identified most often as the short-chain NAHL, N -hexanoyl- l -homoserine lactone, N -(3-oxo-hexanoyl)-homoserine lactone, and N -(3-oxo-octanoyl)- l -homoserine lactone (no absolute correlation between genomospecies of P. syringae and their ability to produce NAHL …

How can a country like Canada be inhospitable to an influence of yardstick competition on regulation?

2007

Expression Patterns of Key Hormones Related to Pea (Pisum sativum L.) Embryo Physiological Maturity Shift in Response to Accelerated Growth Conditions

2019

Protocols have been proposed for rapid generation turnover of temperate legumes under conditions optimized for day-length, temperature, and light spectra. These conditions act to compress time to flowering and seed development across genotypes. In pea, we have previously demonstrated that embryos do not efficiently germinate without exogenous hormones until physiological maturity is reached at 18 days after pollination (DAP). Sugar metabolism and moisture content have been implicated in the modulation of embryo maturity. However, the role of hormones in regulating seed development is poorly described in legumes. To address this gap, we characterized hormonal profiles (IAA, chlorinated auxin…

Networks of Seed Storage Protein Regulation in Cereals and Legumes at the Dawn of the Omics Era

2012

BAP GEAPSI CT2 (BAP); International audience; Finely regulated and orchestrated events occur during seed development and germination in different cell organelles (mitochondria, peroxisomes, plastids, vacuoles, endoplasmic reticulum, oil bodies and nuclei). Therefore, some of the ongoing investigations pass through sub-cellular organelle purifications for a deeper comprehension of these complex developmental processes. In seed biology, recent ambitious efforts in proteomics have been directed toward organelle isolation from seeds and high-throughput protein separation and identification by mass spectrometry at defined developmental stages. The construction of reference maps allowed identifyi…

Effets simultanés de niveaux croissants de glucose et de pressions partielles en oxygène sur la dénitrification et la réduction dissimilative du nitr…

1998

International audience; Dissimilatory nitrate reduction to ammonium (DNRA) and its importance in comparison to denitrification were studied in soil samples artificially repacked to control water potential and porosity, and incubated for 72 h. Labelled nitrate (100 mg N.kg-’ dry soil, 21.8 % 15N in excess) and increasing levels of glucose-C (250, 500 and 1 000 mg glucose-Ckg-’ dry soil) were initially added to the soil samples to obtain increasing glucose-C/nitrate-N ratios of 2.5,5 and 10, which were then subjected to different 0, partial pressures (0,0.5, 1.0 and 2.0 % (v/v)). The results confirmed the good reproducibility of the experimental condi- tions using this method. Denitrification…