Search results for "RFS"
showing 10 items of 49 documents
FUNCTIONAL NONRETENTIVE FECAL SOILING AND STRESSFUL LIFE EVENTS
2019
Introduction: Functional non-retentive fecal soiling (FNRFS), or encopresis without constipation, is a common problem in pediatric age. FNRFS is associated with high levels of distress for both children and parents and with emotional disorders in about 30%-50% of affected children. This study aimed to evaluate stressors on a sample of children with FNRFS comparing to a group of typical devolpment children (TDC). Methods:154 subjects participated in the study: 56 FNRFS children (37 males; mean age 10.87 years ± 1.68); 98 TDC (65 males; mean age 11.3 years ± 1.85). All participants were evaluated for the presence of stressful events (LCU) using the Coddington Life Events Scales (CLES). Result…
Basic phenotypic analysis of six novel yeast genes reveals two essential genes and one which affects the growth rate
1999
Phenotypic analysis was performed on six mutants of Saccharomyces cerevisiae deleted in one of the following open reading frames (ORFs), located on chromosome II: YBR254c, YBR255w, YBR257w, YBR258c, YBR259w and YBR266c. Disruption of the ORFs was carried out in the diploid strain FY1679 using the kanMX4 marker flanked by short sequences homologous to the target locus. Tetrad analysis following sporulation of the heterozygous disruptants showed that YBR254c and YBR257w are essential genes. YBR257w was later characterized and renamed POP4, its gene product being involved in 5.8S rRNA and tRNA processing (Chu et al., 1997). The tetrad analysis performed for the heterozygous disruptant for YBR2…
Functional analysis of 12 ORFs fromSaccharomyces cerevisiae chromosome II
1999
Twelve different ORFs have been deleted from the right arm of Saccharomyces cerevisiae chromosome II; namely YBR193c, YBR194w, YBR197c, YBR198c, YBR201w, YBR203w, YBR207w, YBR209w, YBR210w, YBR211c, YBR217w and YBR228w. Tetrad analysis of heterozygous deletant strains revealed that YBR193c, YBR198c and YBR211c are essential genes for vegetative growth. No effects were detected in any of the haploid deletion mutants for the rest of the ORFs with respect to growth, gross morphology or mating.
The IStron CdISt1 of Clostridium difficile: molecular symbiosis of a group I intron and an insertion element
2003
Abstract The IStron CdISt1 was first discovered as an insertion into the tcdA gene of the clinical isolate C34. It combines structural and functional properties of a group I intron at its 5′-end with those of an insertion element at its 3′-end. Up to date four different types could be found, mainly differing in their IS-element portions. Contrasting classical group I introns, CdISt1 is always integrated in ORFs encoding bacterial protein. In case CdISt1 had only the IS-element function such insertion would inactivate the protein encoded by the host gene. It is only due to the self-splicing activity of the group I intron parts that CdISt1 integration does not abolish protein function. Both e…
A chimeric ribozyme in Clostridium difficile combines features of group I introns and insertion elements
2002
CdlSt1, a DNA insertion of 1975 bp, was identified within tcdA-C34, the enterotoxin gene of the Clostridium difficile isolate C34. Located in the catalytic domain A1-C34, Cd/St1 combines features of two genetic elements. Within the first 434 nt structures characteristic for group I introns were found; encoding the two transposase-like proteins tlpA and tlpB nucleotides 435-1975 represent the remainder of a IS605-like insertion element. We show that the entire CdlSt1 is accurately spliced from tcdA-C34 primary transcripts and that purified TcdA-C34 toxin is of regular size and catalytic activity. A search for CdlSt1-related sequences demonstrates that the element is widespread in toxinogenic…
Transcriptional and Structural Study of a Region of Two Convergent Overlapping Yeast Genes
1999
The exceptionally close packing of many yeast genes and other chromosomal elements raises the question of how those elements are functionally insulated. All published work shows that natural insulators are very effective, but transcriptional interference (TI) occurs if they are mutated or if their natural context is altered. Mechanisms to avoid TI are poorly understood, but are thought to involve an interplay of cis sequences and trans factors in a chromatin context. We have studied the case of two convergent closely packed ORFs (56 bp of separation) in chromosome IX of Saccharomyces cerevisiae. mRNAs from POT1 and YIL161w overlap by up to 115 nt. Convergent transcription causes a small but…
Isolation of a putative prolyl-tRNA synthetase (CaPRS) gene fromCandida albicans
1997
We have isolated a 4·0-kb fragment from a genomic library of Candida albicans which contained two open reading frames (ORFs). One of them is homologous to a prolyl-tRNA synthetase that catalyses the charging of a specific tRNA by proline (CaPRS). A deduced sequence of 575 amino acids representing a polypeptide of 66·2 kDa was determined. A FASTA search indicated that the CaPRSp had an overall similarity of 54·4% with the product of a Saccharomyces cerevisiae ORF (YER087) and 43·8% with the prolyl-tRNA synthetase of Escherichia coli (COLIPRO). Consensus Class II aminoacyl-tRNA synthetase sequences were identified by the PROSITE program. CaPRS was localized to chromosome R of the C. albicans …
The Nucleotide Sequence of a 39 kb Segment of Yeast Chromosome IV: 12 New Open Reading Frames, Nine Known Genes and One Gene for Gly-tRNA
1997
The complete nucleotide sequence of a 39 090 bp segment from the left arm of yeast chromosome IV was determined. Twenty-one open reading frames (ORFs) longer than 100 amino acids and a Gly-tRNA gene were discovered. Nine of the 21 ORFs (D0892, D1022, D1037, D1045, D1057, D1204, D1209, D1214, D1219) correspond to the previously sequenced Saccharomyces cerevisiae genes for the NAD-dependent glutamate dehydrogenase (GDH), the secretory component (SHR3), the GABA transport protein (UGA4), the high mobility group-like protein (NHP2), the hydroxymethylbilane synthase (HEM3), the methylated DNA protein-cysteine S-methyltransferase (MGT1), a putative sugar transport protein, the Shm1 protein (SHM1)…
Paaudžu problēmas Gētes jaunā Vērtera ciešanās un Plencdorfa Jaunā Vērtera jaunās ciešanas
2018
Maģistra darba tēma ir paaudžu problēmas Johana Volfganga fon Gētes romānā ''Jaunā Vertera ciešanas'' un Ulriha Plencdorfa ''Jaunā V. jaunās ciešanas'', pamatojoties uz šo divu darbu salīdzinājumu. Maģistra darba pirmajā daļā ir apskatītas Gētes un Plencdorfa biogrāfijas, lai saprastu, kas stāv aiz šiem bestselleriem. Tālāk abi autori tiek izskatīti kā viņu laika pārstāvji, proti, Gēte kā 18. gadsimta pārstāvis un Plencdorfs kā 20. gadsimta pārstāvis. Analizējot šo tēmu, atklājas, ka abas grāmatas bija ļoti populāras lasītāju vidū, taču valdības un tās pārstāvju viedoklis nebija pozitīvs. Darba otrajā daļā ir apskatīti vēsturiski notikumi Gētes dzīves laikā un pirms grāmatas sastādīšanas, t…
Deployment of a WLCG network monitoring infrastructure based on the perfSONAR-PS technology
2014
The WLCG infrastructure moved from a very rigid network topology, based on the MONARC model, to a more relaxed system, where data movement between regions or countries does not necessarily need to involve T1 centres. While this evolution brought obvious advantages, especially in terms of flexibility for the LHC experiment's data management systems, it also opened the question of how to monitor the increasing number of possible network paths, in order to provide a global reliable network service. The perfSONAR network monitoring system has been evaluated and agreed as a proper solution to cover the WLCG network monitoring use cases: it allows WLCG to plan and execute latency and bandwidth te…