Search results for "RIBOFLAVIN"
showing 10 items of 40 documents
Efficacy of systemic administration of riboflavin on a rabbit model of corneal alkali burn
2020
AbstractChanges in the barrier mechanisms in the eye should determine the rational route for the administration and dosage of each drug in the treatment of traumatic injuries and other pathologies. The aim of this study was to examine the efficacy of intra-arterial delivery of 14C-riboflavin (as an “indicator”) and compare it with intravenous and intramuscular administration in an animal model of chemical eye burn. 14C-riboflavin (14C-I) was administered by intra-arterial (carotid artery), intravenous (femoral vein) and intramuscular (femoral muscle) routes. The total radioactivity was determined over 2 h in the plasma and structures of the rabbit’s eyes using a scintillation counter. The r…
Real-time detection of riboflavin production by Lactobacillus plantarum strains and tracking of their gastrointestinal survival and functionality in …
2019
Some strains of lactic acid bacteria (LAB) produce riboflavin, a water-soluble vitamin of the B complex, essential for human beings. Here, we have evaluated riboflavin (B2 vitamin) production by five Lactobacillus plantarum strains isolated from chicha, a traditional maize-based fermented alcoholic beverage from north-western Argentina and their isogenic riboflavin-overproducing derivatives previously selected using roseoflavin. A direct fluorescence spectroscopic detection method to quantify riboflavin production in bacterial culture supernatants has been tested. Comparison of the efficiency for riboflavin fluorescence quantification with and without prior HPLC fractionation showed that th…
The Ability of Riboflavin-Overproducing Lactiplantibacillus plantarum Strains to Survive Under Gastrointestinal Conditions
2020
17 p.-5 fig.-4 tab.+mat. supl.:5 p.-3 fig.-1 tab.
Genomic Insights Into Five Strains of Lactobacillus plantarum With Biotechnological Potential Isolated From chicha, a Traditional Maize-Based Ferment…
2019
Lactic acid bacteria (LAB) are indigenous microorganisms that have been involved in food fermentations throughout history to preserve food and also to give special characteristics to them. The traditional fermented foods that are still being elaborated in indigenous populations around the world are a potential source of LAB with important biotechnological properties and/or beneficial to health. In a previous work, LAB biodiversity associated with chicha, a traditional maize-based fermented beverage from Northwestern Argentina, was studied, both by culture dependent and independent methods. From that study, 392 isolates were recovered, mostly members of Lactobacillus and Leuconostoc. Biotech…
Simultaneous Determination of Thiamin and Riboflavin in Mushrooms by Liquid Chromatography
2001
A simple, fast, inexpensive, and reliable method useful for the simultaneous, routine determination of thiamin and riboflavin in mushrooms is examined. It uses the extraction procedure, with slight modifications, proposed by the AOAC for the extraction of thiamin and riboflavin, followed by a liquid chromatographic separation on a reversed-phase Spherisorb ODS column with methanol/water as mobile phase gradient. Fluorometric detection is used at the following excitation and emission wavelengths, respectively, 360 and 425 nm in the case of thiamin and 422 and 515 nm for riboflavin. The analytical parameters of linearity, the precision of the method (RSD = 2.45 and 2.51% for thiamin and ribof…
Maternal vitamin deficiency mimicking multiple acyl-CoA dehydrogenase deficiency on newborn screening
2021
Abstract Background In infancy multiple acyl-CoA dehydrogenase deficiency (MADD) is commonly a severe inherited metabolic disease caused by genetic defects in electron transfer flavoprotein (ETF) or ETF ubiquinone oxidoreductase. Both enzymes require flavin adenine dinucleotide (FAD) as a cofactor. Riboflavin (vitamin B2) is a precursor in the synthesis of FAD. MADD can be detected by newborn screening (NBS) based on elevation of multiple acylcarnitines. Methods We present the results of two children whose NBS results and subsequent confirmatory testing resulted in a suspected diagnosis of MADD. In parallel in both children vitamin B12 deficiency was detected. Results Biochemical profiles n…
Zur Frage der Schutzwirkung von Thiamin bei chronischer Alkoholintoxikation
1960
Ratten wurden nach Entwohnung in 3 Gruppen zu je 42–50 Tieren auf folgende Weise ernahrt: a) die Kontrolltiere erhielten eine leberschadigende Kost und Trinkwasser, b) die Tiere der Gruppe I leberschadigende Kost und 16%igen Alkohol, dazu von der 5. Versuchswoche an 40 γ Thiamin pro Tier und Tag, d. i. die zehnfache Normaldosis, c) diejenigen der Gruppe II leberschadigende Diat und Alkohol.
Vitamin A-Wachstums- und Leberspeichertest bei Ratten, mit gleichzeitiger Prüfung eines wasser- und fettlöslichen Präparates
1960
1. Wir fuhrten den Vitamin A-Wachstumstest mit dem wasserloslichen Palmitinsaureester und dem fettloslichen Vitamin A-Acetat durch. Die Tagesdosis betrug 1,5 bzw. 2,5 IE/Tier. Jede der 4 Gruppen bestand aus 30 Mannchen. Das Ergebnis wurde nach dem t-Test vonStudent ausgewertet. 2. Unter den Bedingungen unseres Experimentes erhoht der wasserlosliche Palmitinsaureester das Wachstum gegenuber dem fettloslichen A-Acetat signifikant. Die Differenz ist bei der geringen Dosis von 1,5 IE am grosten. Der Einflus des Futterverzehrs auf das Wachstum wird diskutiert. 3. Beim Leberspeichertest arbeiteten wir wieder mit 4 Rattengruppen zu je 30 Tieren. Verwendet wurden die gleichen Praparate wie beim Wac…
Enzymatic cross-linking of pea proteins to produce microparticles : application to the encapsulation of riboflavin
2015
In this work, pea proteins behavior toward enzymatic gelation by microbial transglutaminase (MTGase) was studied at native state and after denaturation (chemical reduction or thermal denaturation). The final application was the formation of protein microparticules to encapsulate riboflavin, chosen as hydrophilic active molecule model. The extraction process of the pea protein fractions has been optimized in such a way to minimize as possible protein denaturation and recover native fractions rich in albumin (Alb) and globulin (Glob) or a mixture of both.The setting up of the enzymatic reaction monitoring methods has brought out their complementarity as well as their limits. Two new monitorin…
Influence of Riboflavin Targeting on Tumor Accumulation and Internalization of Peptostar Based Drug Delivery Systems.
2020
Riboflavin carrier protein (RCP) and riboflavin transporters (RFVTs) have been reported to be highly overexpressed in various cancer cells. Hence, targeting RCP and RFVTs using riboflavin may enhance tumor accumulation and internalization of drug delivery systems. To test this hypothesis, butyl-based 3-arm peptostar polymers were synthesized consisting of a lysine core (10 units per arm) and a sarcosine shell (100 units per arm). The end groups of the arms and the core were successfully modified with riboflavin and the Cy-5.5 fluorescent dye, respectively. While in phosphate buffered saline the functionalized peptostars showed a bimodal behavior and formed supramolecular structures over tim…